Volume 23 Issue 4
Jul.  2002
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WEI Qin, LI Rui, WANG Wan-yu, XIONG Yu-liang. Cloning and Sequence Analysis of cDNAs Encoding Four C-type Lectin-like Proteins from Snake Trimeresurus mucrosquamatus. Zoological Research, 2002, 23(4): 273-279.
Citation: WEI Qin, LI Rui, WANG Wan-yu, XIONG Yu-liang. Cloning and Sequence Analysis of cDNAs Encoding Four C-type Lectin-like Proteins from Snake Trimeresurus mucrosquamatus. Zoological Research, 2002, 23(4): 273-279.

Cloning and Sequence Analysis of cDNAs Encoding Four C-type Lectin-like Proteins from Snake Trimeresurus mucrosquamatus

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2002-08-22
  • The total mRNA was prepared and purified from the venom glands of snake Trimer esurus mucrosquamatus.The cDNAs encoding C-type lectin-like proteins were amplified by RT-PCR and cloned into the PMD 18-T vectors.The positive clones encoding four C-type lectin-like proteins (TML-1,TML-2,TML-3 and TML-4) were selected and sequenced.Amino acid sequences of these proteins were deduced and each contains carbohydraterecognition domain (CRD).Homology comparison and cystein position analysis indicate that TML-1 and TML-2 might be the α and β subunits of a C-type lectin-like protein similar to flavocetin-A,TML-3 the α subunit of a C-type lectin-like protein similar to GPIb-bp,and TML-4 the β subunit of a C-type lectin-like protein similar to Ⅸ/Ⅹ-bp.
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Cloning and Sequence Analysis of cDNAs Encoding Four C-type Lectin-like Proteins from Snake Trimeresurus mucrosquamatus

Abstract: The total mRNA was prepared and purified from the venom glands of snake Trimer esurus mucrosquamatus.The cDNAs encoding C-type lectin-like proteins were amplified by RT-PCR and cloned into the PMD 18-T vectors.The positive clones encoding four C-type lectin-like proteins (TML-1,TML-2,TML-3 and TML-4) were selected and sequenced.Amino acid sequences of these proteins were deduced and each contains carbohydraterecognition domain (CRD).Homology comparison and cystein position analysis indicate that TML-1 and TML-2 might be the α and β subunits of a C-type lectin-like protein similar to flavocetin-A,TML-3 the α subunit of a C-type lectin-like protein similar to GPIb-bp,and TML-4 the β subunit of a C-type lectin-like protein similar to Ⅸ/Ⅹ-bp.

WEI Qin, LI Rui, WANG Wan-yu, XIONG Yu-liang. Cloning and Sequence Analysis of cDNAs Encoding Four C-type Lectin-like Proteins from Snake Trimeresurus mucrosquamatus. Zoological Research, 2002, 23(4): 273-279.
Citation: WEI Qin, LI Rui, WANG Wan-yu, XIONG Yu-liang. Cloning and Sequence Analysis of cDNAs Encoding Four C-type Lectin-like Proteins from Snake Trimeresurus mucrosquamatus. Zoological Research, 2002, 23(4): 273-279.

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