Volume 23 Issue 1
Jan.  2002
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HAN Zhao-jiu, HAN Zhao-jun. Cloning and Sequence of Nicotinic Acetylcholine Receptor α Subunit from Chilo suppressalis. Zoological Research, 2002, 23(1): 7-13.
Citation: HAN Zhao-jiu, HAN Zhao-jun. Cloning and Sequence of Nicotinic Acetylcholine Receptor α Subunit from Chilo suppressalis. Zoological Research, 2002, 23(1): 7-13.

Cloning and Sequence of Nicotinic Acetylcholine Receptor α Subunit from Chilo suppressalis

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2002-02-22
  • Nicotinic acetylcholine receptors (nAChRs) play a significant role in excitatory synaptic transmission in insects and are the target for chloronicotinyl and nereistoxin insecticides.In recent years,Chilo suppressalis,an economically important pest of rice,developed high resistance against monosultap,a nereistoxin insecticide acting on nAChR.In order to reveal the hypothesized target insensitive mechanism,studies on the molecular property of nAChR from Chilo suppressalis are required.In this study,the full length cDNA of nAChR α subunit from this pest was cloned by RT-PCR.Sequence analysis shows that it is a novel nAChR α subunit,which was named as Cs α 1(Genbank accession No.AF418987).It contains 1 997 bp nucleotides and involves an open reading frame (ORF) encoding a mat ure protein of 509 amino acids excluding a signal peptide of 24 amino acids.The deduced amino acid sequence was 52%-94% identical to the reported insect nAChR genes.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Cloning and Sequence of Nicotinic Acetylcholine Receptor α Subunit from Chilo suppressalis

Abstract: Nicotinic acetylcholine receptors (nAChRs) play a significant role in excitatory synaptic transmission in insects and are the target for chloronicotinyl and nereistoxin insecticides.In recent years,Chilo suppressalis,an economically important pest of rice,developed high resistance against monosultap,a nereistoxin insecticide acting on nAChR.In order to reveal the hypothesized target insensitive mechanism,studies on the molecular property of nAChR from Chilo suppressalis are required.In this study,the full length cDNA of nAChR α subunit from this pest was cloned by RT-PCR.Sequence analysis shows that it is a novel nAChR α subunit,which was named as Cs α 1(Genbank accession No.AF418987).It contains 1 997 bp nucleotides and involves an open reading frame (ORF) encoding a mat ure protein of 509 amino acids excluding a signal peptide of 24 amino acids.The deduced amino acid sequence was 52%-94% identical to the reported insect nAChR genes.

HAN Zhao-jiu, HAN Zhao-jun. Cloning and Sequence of Nicotinic Acetylcholine Receptor α Subunit from Chilo suppressalis. Zoological Research, 2002, 23(1): 7-13.
Citation: HAN Zhao-jiu, HAN Zhao-jun. Cloning and Sequence of Nicotinic Acetylcholine Receptor α Subunit from Chilo suppressalis. Zoological Research, 2002, 23(1): 7-13.

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