Volume 22 Issue 4
Jul.  2001
Turn off MathJax
Article Contents
WANG Chong, ZHANG Shou-Quan, YANG Guan-Fu. Glucose Metabolism During Kunming Mouse Preimplantation Development:Analysis of Gene Transcription in Embryos in Vivo. Zoological Research, 2001, 22(4): 340-344.
Citation: WANG Chong, ZHANG Shou-Quan, YANG Guan-Fu. Glucose Metabolism During Kunming Mouse Preimplantation Development:Analysis of Gene Transcription in Embryos in Vivo. Zoological Research, 2001, 22(4): 340-344.

Glucose Metabolism During Kunming Mouse Preimplantation Development:Analysis of Gene Transcription in Embryos in Vivo

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2001-08-22
  • In order to investigate glucose metabolism pathways and their changes in Kunming mouse preimplantation 1-,2-,4-,8-cell,and morula embryos,the mRNA level for the genes involved in glucose metabolism was tested by nested RT-PCR on embryos at different development stages in vivo.These genes were glucose 6-phosphate dehydrogenase (G6PDH),phospho-fructokinase (PFK),and phosphoglucomutase (PGM),representing pentose phosphate pathway (PPP),glycolysis,and glycogensis and glycogenolysis respectively.Three sets of inner and outer primers were designed and synthesized based on cDNA sequences of G6PDH,PFK and PGM.RT-PCR results revealed that G6PDH gene transcription was found in Kunming mouse 1-8 cell embryos,and not in morula embryos;it indicated that 1-8 cell embryos may metabolize glucose by pentose phosphate pathway,but morula embryos can not do so.PFK gene transcription was found in 1-8 cell and morula embryos;it is probable that there exists glycolysis in those embryos.PGM gene transcription was not found in 1-8 cell and morula embryos,so glycogenesis and glycogenolysis in these embryos were not present.
  • loading
  • Relative Articles

    [1] Da-Jian He, Lin Wang, Zhi-Bi Zhang, Kun Guo, Jing-Zheng Li, Xie-Chao He, Qing-Hua Cui, Ping Zheng. Maternal gene Ooep may participate in homologous recombination-mediated DNA double-strand break repair in mouse oocytes. Zoological Research, 2018, 39(6): 387-395.  doi: 10.24272/j.issn.2095-8137.2018.067
    [2] SHI Mei-Lian, XU Ping, YIN Xiao-Shu, YANG Wei-Wei, GU Mei-Er, YU LI-Ping, LIU Gui-Jie, WU Bao-Jin. Phenotype analysis and mutant gene location of ventral yellow mouse (VYSlac). Zoological Research, 2012, 33(3): 290-297.  doi: 10.3724/SP.J.1141.2012.03290
    [3] TIAN Wei-Wei, GAO Yue-Dong, GUO Yan, HUANG Jing-Fei, XIAO Chang, LI Zuo-Sheng, ZHANG Hua-Tang. Cloning of full-length coding sequence of tree shrew CD4 and prediction of its molecular characteristics. Zoological Research, 2012, 33(1): 60-66.  doi: 10.3724/SP.J.1141.2012.01060
    [4] ZHOU Rui-Xue MENG Tao, Meng Hai-Bo, HENG Dun-Xue BIN Shi-Yu CHENG Jia, FU Gui-Hong, CHU Wu-Ying, *, ZHANG Jian-She , *. Selection of Reference Genes in Transcription Analysis of Gene Expression of the Mandarin Fish, Siniperca chuasti. Zoological Research, 2010, 31(2): 141-146.  doi: 10.3724/SP.J.1141.2010.02141
    [5] ZHANG Ying, JIN Shan, ZHAO Qing-Song, WANG Guo-Liang, YU Kai, WANG Chun-Lin. Cloning and expression pattern analysis of a lipopolysaccharide -and β-1,3-glucan-binding protein in Portunus trituberculatus. Zoological Research, 2010, 31(6): 587-594.  doi: 10.3724/SP.J.1141.2010.06587
    [6] WU Bao-jin, MAO Hui-hua, ZENG Yong-mei, YIN Li-jing, YIN Xiao-shu, YANG Wei-wei, K. Fine Mapping and Identifying the Mutation Gene of snthr-1Bao Scant Hair Mouse. Zoological Research, 2009, 30(3): 267-275.  doi: 10.3724/SP.J.1141.2009.03267
    [7] BAI Yin-ba-tu, LUO Fen-hua, HU Tian-yuan, HOU Yue, WU Ying-ji. Cloning of Sheep tnp2 Gene and Transcription Analysis of Round Spermatid Cells in the in vitro Co-culture System. Zoological Research, 2009, 30(3): 262-266.  doi: 10.3724/SP.J.1141.2009.03262
    [8] ZHANG Yu-jun, MAO Bing-yu. Developmental Expression of an Amphioxus (Branchiostoma belcheri) Gene Encoding a GATA Transcription Factor. Zoological Research, 2009, 30(2): 137-143.  doi: 10.3724/SP.J.1141.2009.02137
    [9] WANG Jing-jing, NIE Liu-wang, JIA Rui, WANG Ning. cDNA Cloning and Expression Analysis of Mest Gene in the Bufo gargarizans. Zoological Research, 2009, 30(4): 369-376.  doi: 10.3724/SP.J.1141.2009.04369
    [10] MA Li, ZHAO Shu-hua, KONG Qing-hua, MAO Bing-yu, *. Temporal and Spatial Expression Patterns of Sox1 Gene in Xenopus laevis Embryo. Zoological Research, 2007, 28(4): 403-408.
    [11] DING Fang, ZHOU Hong-lin, LIU Yang, MA Lan, SU Ying, DU Ling. Effects of Glucose on Development of ICR Mouse Embryosin vitro. Zoological Research, 2007, 28(5): 501-506.
    [12] GUAN Mo, YANG Shi-hua, SI Wei, CAI Ke-jun, JI Wei-zhi. Effect of Bovine Serum Albumin on Vitrification of Pronuclear-Stage Mouse Embryo. Zoological Research, 2005, 26(2): 197-202.
    [13] CHEN Jin-ping, YUAN Hong-mei, WANG Bin, LIANG Bing, ZHANG Shu-yi. cDNA Cloning,mRNA Transcription of Sox9 Gene at Early Developmental Stages in Amur Sturgeons (Acipenser schrenckii). Zoological Research, 2004, 25(6): 527-533.
    [14] LIU Xu-sheng, LIANG Bing, ZHANG Shu-yi. cDNA Cloning,Tissue Distribution and mRNA Transcription of DMRT1 Gene in the Protandrous Black Porgy Acanthopagrus schlegeli. Zoological Research, 2004, 25(2): 158-161.
    [15] ZHAO Tong-biao, ZHAO Xin-quan, CHANG Zhi-jie, SUN Ping, XU Shi-xiao, ZHAO Wei. Tissue Specific Expression of Plateau Pikas (Ochotona curzoniae) HIF-1α mRNA Under Normal Oxygen. Zoological Research, 2004, 25(2): 132-136.
    [16] AI Xiao-jie, ZHENG Yuan-lin, CHEN Wei-hua, HAN Zheng-kang. Effects of Cysteamine on the Metabolism of Sugar and Protein in Adult Geese. Zoological Research, 2003, 24(4): 302-304.
    [17] YOU Wen-hui, WANG Pei-chao. A Study on The Yolk Metabolism of Embryos and Hatchlings in Chinemys reevesii. Zoological Research, 1994, 15(3): 45-50.
    [18] ZHOU Rong-jia, ZHANG Si-zhong, YANG Jun, TANG Yong-cai. PCR Amplification and cloning of A Gene of Cattle (Bos taurus) Homologous To Human Sry. Zoological Research, 1993, 14(3): 252-257.
    [19] HU Jia-xiang, LIN Shi-yin, LIU Ai-hua, ZHANG Han-yun. Gentical effect of Surfactants on Sce Formation of Mouse Bone Marrow Cells and Gene Mutation of Salmonella Typhimurium. Zoological Research, 1992, 13(1): 83-88.
    [20] CHEN Yun-he, MEN Hong-sheng, ZHOU Xin-liang, HE Xue-chao, DING Kai-yong, MU Tong-xian, LI Tian-ping. The Study of Bovine Embryo Transfer in Yunnan. Zoological Research, 1991, 12(4): 381-385.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1970) PDF downloads(1604) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return