Volume 22 Issue 4
Jul.  2001
Turn off MathJax
Article Contents
ZHANG Hai-Jun, NIE Liu-Wang, SHAN Xiang-Nian, ZHANG Xiao-Ai. Cloning and Sequencing of the Sox Genes in Chinemys reevesii. Zoological Research, 2001, 22(4): 336-339.
Citation: ZHANG Hai-Jun, NIE Liu-Wang, SHAN Xiang-Nian, ZHANG Xiao-Ai. Cloning and Sequencing of the Sox Genes in Chinemys reevesii. Zoological Research, 2001, 22(4): 336-339.

Cloning and Sequencing of the Sox Genes in Chinemys reevesii

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2001-08-22
  • Using a pair of degenerate primers based on the conservative region,HMG-box,of human SRY gene,four different fragments were amplified from both female and male Chinemys reevesii,then cloned by using pGEM-T vector and sequenced.The sequence result indicated their high homology to human Sox genes.The identities to human Sox genes in the DNA sequence and the amino acid sequence are 92%,91%,84%,92% and 100%,93%,98%,98%,respectively.It might be conclud ed that Sox gene was highly conservative in phylogenesis.Our work provides molecular data for the study of sex-determining mechanism of C.reevesii,which might be temperature-dependent sex determination.
  • loading
  • Relative Articles

    [1] Hong-Mei ZHANG, Yan-Fang SU, Zhi-Yi SHI, Yuan-Shuai FU. cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis. Zoological Research, 2014, 35(4): 307-312.  doi: 10.13918/j.issn.2095-8137.2014.4.307
    [2] Xiao-Yan HUANG, Ming-Li LI, Juan XU, Yue-Dong GAO, Wen-Guang WANG, An-Guo YIN, Xiao-Fei LI, Xiao-Mei SUN, Xue-Shan XIA, Jie-Jie DAI. Analysis of the molecular characteristics and cloning of full-length coding sequence of Interleukin-2 in tree shrews. Zoological Research, 2013, 34(2): 121-126.  doi: 10.3724/SP.J.1141.2013.02121
    [3] Jinling CAO, Jianjie CHEN, Zhongliang JIANG, Yongju LUO, Xi GAN. Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus. Zoological Research, 2012, 33(E3-4): 40-46.  doi: 10.3724/SP.J.1141.2012.E03-04E40
    [4] WANG Fang, CHEN Jiong, SHI Yu-Hong, LU Xin-Jiang, LI Ming-Yun. Molecular cloning, sequence analysis and stress-related changes of the heat shock protein 60 gene in Neobenedenia melleni. Zoological Research, 2012, 33(6): 603-608.  doi: 10.3724/SP.J.1141.2012.06603
    [5] LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 2010, 31(5): 483-489.  doi: 10.3724/SP.J.1141.2010.05483
    [6] YUAN Feng, MIAO Yong-Wang, LI Da-Lin, TANG Shou-Kun, Xü Zheng, HUO Jin-Long, QI Hong. Genetic characteristics on exon 4 of prolactin gene in 12 water buffalo populations. Zoological Research, 2010, 31(6): 575-580.  doi: 10.3724/SP.J.1141.2010.06575
    [7] WANG Jing-jing, NIE Liu-wang, JIA Rui, WANG Ning. cDNA Cloning and Expression Analysis of Mest Gene in the Bufo gargarizans. Zoological Research, 2009, 30(4): 369-376.  doi: 10.3724/SP.J.1141.2009.04369
    [8] HOU Yue, WU Ying-ji, LUO Fen-hua, SU Hui-min, ZHANG Xue-ming, ZHANG Yan, BAI Yin-ba-tu, LIU Tao-di. Cloning of Cashmere Goat Scp3 Gene and Determining the First Round Meiosis in the Testes. Zoological Research, 2009, 30(3): 341-344.  doi: 10.3724/SP.J.1141.2009.03341
    [9] BAI Yin-ba-tu, LUO Fen-hua, HU Tian-yuan, HOU Yue, WU Ying-ji. Cloning of Sheep tnp2 Gene and Transcription Analysis of Round Spermatid Cells in the in vitro Co-culture System. Zoological Research, 2009, 30(3): 262-266.  doi: 10.3724/SP.J.1141.2009.03262
    [10] YANG Xiu-qin, GUO Li-juan, MA Jian-zhang, LIU Di. Cloning, Expression and Variation Analysis of the Wild Boar CAPN7 Gene. Zoological Research, 2009, 30(5): 503-508.  doi: 10.3724/SP.J.1141.2009.05503
    [11] XU Qing-gang, CHEN Ke-ping, YAO Qin, LIU Xiao-yong. Screening and Cloning of RAPD Marker of Fluoride Tolerance Gene in Silkworm,Bombyx mori. Zoological Research, 2004, 25(1): 69-72.
    [12] CHEN Jin-ping, YUAN Hong-mei, WANG Bin, LIANG Bing, ZHANG Shu-yi. cDNA Cloning,mRNA Transcription of Sox9 Gene at Early Developmental Stages in Amur Sturgeons (Acipenser schrenckii). Zoological Research, 2004, 25(6): 527-533.
    [13] LI Chang-jian, LIU Jun, SHI Yao-hua, YANG Shu-ting, GUI Jian-fang. Full Length cDNA Cloning and Tissue Expression Specificity of Ran Gene in Color Crucian Carp. Zoological Research, 2003, 24(3): 173-179.
    [14] CHEN Dong-sheng, NIE Liu-wang, CHENG Shuang-huai, KAN Xian-zhao, WANG Chao-lin, XIE Wan-shu. Amplification and Sequencing of Sox Gene HMG-box in Alligator sinensis. Zoological Research, 2003, 24(2): 127-131.
    [15] DONG Yun-wei, NIU Cui-juan, BAO Lei, LI Qing-fen, HUANG Chen-xi. Method for Extracting DNA from Single Rotifer and Sequencing Partical Mitochondria Cytochrome Oxidase Subunit Ⅰ (COⅠ) Gene. Zoological Research, 2002, 23(1): 81-83.
    [16] LI Fei, HAN Zhao-jun. Cloning and Sequencing of Two Acetylcholinesterase cDNA Fragments from Cotton Aphid,Aphis gossypii Glover. Zoological Research, 2002, 23(5): 444-448.
    [17] YOU Wen-hui, WANG Pei-chao. A Study on The Yolk Metabolism of Embryos and Hatchlings in Chinemys reevesii. Zoological Research, 1994, 15(3): 45-50.
    [18] ZHOU Rong-jia, ZHANG Si-zhong, YANG Jun, TANG Yong-cai. PCR Amplification and cloning of A Gene of Cattle (Bos taurus) Homologous To Human Sry. Zoological Research, 1993, 14(3): 252-257.
    [19] YOU Wen-hui, LU Bo, WANG Pei-chao. The Effect of Ambient Temperature and Kinds of Food on Growth and Digestion of Juvenile Turtles,Chinemys reevesll. Zoological Research, 1993, 14(2): 136-142.
    [20] LIU Guo-an, LIU Yun-qing, HU Di-guang, XIONG Wen-hua. A Preliminary Observation on the Development of the Embryo of Tortoise,Chinemys reevesii. Zoological Research, 1984, 5(1): 51-56.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1915) PDF downloads(1526) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint