Volume 19 Issue 1
Jan.  1998
Turn off MathJax
Article Contents

FXU Le-ren, JIANG Ping. Histochemical Techniques Identifying Mast Cells in Pig,Cattle and Sheep. Zoological Research, 1998, 19(1): 1-10.
Citation: FXU Le-ren, JIANG Ping. Histochemical Techniques Identifying Mast Cells in Pig,Cattle and Sheep. Zoological Research, 1998, 19(1): 1-10.

Histochemical Techniques Identifying Mast Cells in Pig,Cattle and Sheep

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1998-02-22
  • Carnoys fluid and neutral buffered formalin(NBF)have been proved to be good fixatives for preservation of mast cells in pig,cattle and sheep except NBF blocked staining of most porcine mast cells,especially those located in intestinal mucosa (MMC) and in thymus medulla(TMMC).Both toluidine blue and Alcian blue were the excellent stains generally,but Alcian blue stained more porcine mast cells than did toluidine blue (P<0.01).Staining with toluidine blue of a wide pH range(from 0.1 to 7.0)showed that porcine mast cells were not very pH dependent,but the dye at pH 0.5 seemed to have the strongest affinity for all mast cells in pigs and it was also suitable for bovine and ovine mast cell staining.In the three species,unlike in rodents,the Alcian blue method did not distinguish between mast cells in the intestinal mucosa (MMC) and those in the connective tissue of the intestinal submucosa,tongue and skin (CTMC).Porcine CTMC,but not MMC,fluoresced strongly when stained with berberine sulphate or with a mixture of berberine sulphate and acridine orange.It suggested that porcine CTMC contained heparin proteoglycan.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1964) PDF downloads(1332) Cited by()

Related
Proportional views

Histochemical Techniques Identifying Mast Cells in Pig,Cattle and Sheep

Abstract: Carnoys fluid and neutral buffered formalin(NBF)have been proved to be good fixatives for preservation of mast cells in pig,cattle and sheep except NBF blocked staining of most porcine mast cells,especially those located in intestinal mucosa (MMC) and in thymus medulla(TMMC).Both toluidine blue and Alcian blue were the excellent stains generally,but Alcian blue stained more porcine mast cells than did toluidine blue (P<0.01).Staining with toluidine blue of a wide pH range(from 0.1 to 7.0)showed that porcine mast cells were not very pH dependent,but the dye at pH 0.5 seemed to have the strongest affinity for all mast cells in pigs and it was also suitable for bovine and ovine mast cell staining.In the three species,unlike in rodents,the Alcian blue method did not distinguish between mast cells in the intestinal mucosa (MMC) and those in the connective tissue of the intestinal submucosa,tongue and skin (CTMC).Porcine CTMC,but not MMC,fluoresced strongly when stained with berberine sulphate or with a mixture of berberine sulphate and acridine orange.It suggested that porcine CTMC contained heparin proteoglycan.

FXU Le-ren, JIANG Ping. Histochemical Techniques Identifying Mast Cells in Pig,Cattle and Sheep. Zoological Research, 1998, 19(1): 1-10.
Citation: FXU Le-ren, JIANG Ping. Histochemical Techniques Identifying Mast Cells in Pig,Cattle and Sheep. Zoological Research, 1998, 19(1): 1-10.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return