Volume 27 Issue 4
Jul.  2006
Turn off MathJax
Article Contents

LI Xue-yan, YANG Shuang, LIANG Xing-cai , *. Phylogenetic Relationship of the Firefly, Diaphanes pectinealis (Insecta, Coleoptera, Lampyridae) Based on DNA Sequence and Gene Structure of Luciferase. Zoological Research, 2006, 27(4): 367-374.
Citation: LI Xue-yan, YANG Shuang, LIANG Xing-cai , *. Phylogenetic Relationship of the Firefly, Diaphanes pectinealis (Insecta, Coleoptera, Lampyridae) Based on DNA Sequence and Gene Structure of Luciferase. Zoological Research, 2006, 27(4): 367-374.

Phylogenetic Relationship of the Firefly, Diaphanes pectinealis (Insecta, Coleoptera, Lampyridae) Based on DNA Sequence and Gene Structure of Luciferase

  • Received Date: 2006-01-12
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2006-08-22
  • Diaphanes is the fourth largest genus in Lampyridae, but no luciferase gene from this genus has been reported. In this paper, by PCR amplification of the genomic DNA, the luciferase gene of Diaphanes pectinealis, which is the first case from Diaphanes, was identified and sequenced. The luciferase gene from D. pectinealis spans 1958 base pairs (bp) from the start to the stop codon, including seven exons separated by six introns, and encoding a 547-residuelong polypeptide. Its deduced amino acid sequence showed high protein similarity to those of the Lampyrini tribe (93-94%) and the Cratomorphini tribe (92%), while low similarity was found with the North American firefly Photinus pyralis (83%) of the Photinini tribe within the same subfamily Lampyrinae. The phylogenetic analysis performed with the deduced amino acid sequences of the luciferase gene further confirms that D. pectinealis, Pyrocoelia, Lampyris, Cratomorphus, and Photinus belong to the same subfamily Lampyrinae, and Diaphanes is closely related to Pyrocoelia, Lampyris, and Cratomorphus. Furthemore, the phylogenetic analysis based on the nucleotide sequences of the luciferase gene indicates Diaphanes is a sister to Lampyris. The phylogenetic analyses are partly consistent with morphological (Branham & Wenzel, 2003) and mitochondrial DNA analyses (Li et al, 2006).
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(2319) PDF downloads(1771) Cited by()

Related
Proportional views

Phylogenetic Relationship of the Firefly, Diaphanes pectinealis (Insecta, Coleoptera, Lampyridae) Based on DNA Sequence and Gene Structure of Luciferase

Abstract: Diaphanes is the fourth largest genus in Lampyridae, but no luciferase gene from this genus has been reported. In this paper, by PCR amplification of the genomic DNA, the luciferase gene of Diaphanes pectinealis, which is the first case from Diaphanes, was identified and sequenced. The luciferase gene from D. pectinealis spans 1958 base pairs (bp) from the start to the stop codon, including seven exons separated by six introns, and encoding a 547-residuelong polypeptide. Its deduced amino acid sequence showed high protein similarity to those of the Lampyrini tribe (93-94%) and the Cratomorphini tribe (92%), while low similarity was found with the North American firefly Photinus pyralis (83%) of the Photinini tribe within the same subfamily Lampyrinae. The phylogenetic analysis performed with the deduced amino acid sequences of the luciferase gene further confirms that D. pectinealis, Pyrocoelia, Lampyris, Cratomorphus, and Photinus belong to the same subfamily Lampyrinae, and Diaphanes is closely related to Pyrocoelia, Lampyris, and Cratomorphus. Furthemore, the phylogenetic analysis based on the nucleotide sequences of the luciferase gene indicates Diaphanes is a sister to Lampyris. The phylogenetic analyses are partly consistent with morphological (Branham & Wenzel, 2003) and mitochondrial DNA analyses (Li et al, 2006).

LI Xue-yan, YANG Shuang, LIANG Xing-cai , *. Phylogenetic Relationship of the Firefly, Diaphanes pectinealis (Insecta, Coleoptera, Lampyridae) Based on DNA Sequence and Gene Structure of Luciferase. Zoological Research, 2006, 27(4): 367-374.
Citation: LI Xue-yan, YANG Shuang, LIANG Xing-cai , *. Phylogenetic Relationship of the Firefly, Diaphanes pectinealis (Insecta, Coleoptera, Lampyridae) Based on DNA Sequence and Gene Structure of Luciferase. Zoological Research, 2006, 27(4): 367-374.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return