Volume 27 Issue 3
May  2006
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LI Yi , , HUANG Wei, ZHANG Xin, SU Bing , *. Construction of a cDNA Library of the Prefrontal Cortex of Rhesus Monkey. Zoological Research, 2006, 27(3): 325-330.
Citation: LI Yi , , HUANG Wei, ZHANG Xin, SU Bing , *. Construction of a cDNA Library of the Prefrontal Cortex of Rhesus Monkey. Zoological Research, 2006, 27(3): 325-330.

Construction of a cDNA Library of the Prefrontal Cortex of Rhesus Monkey

  • Received Date: 2006-01-12
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2006-06-22
  • Total RNA was extracted from the prefrontal cortex (PFC) of rhesus monkeys with TRIzol reagent. mRNA was further purified from total RNA using a purifying kit for mRNA. According to the manual of cDNA Synthesis Kit (200401), ZAP-cDNA Synthesis Kit (200400),and ZAP-cDNA GigapackⅢ Gold Cloning Kit (200450) from Stratagene, we constructed a cDNA-library of the PFC of rhesus monkeys. Our aim was to identify brain-function (such as cognition and memory) related genes and gene families. The cDNA library has a capacity of 2.0×10 6 clones, and 97.3% of them are recombinant clones. The average size of the inserted cDNA fragments is 1.0 kb with the majority of inserts larger than 0.5 kb, which meets the requirement of a standard cDNA library. The PFC cDNA library constructed will be useful in cloning and sequencing brain-function related genes, and in dissecting the alternative splicing patterns of genes expressed in the brain. Meanwhile, it will also serve as a useful resource in understanding the expression regulations within the brain.
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Construction of a cDNA Library of the Prefrontal Cortex of Rhesus Monkey

Abstract: Total RNA was extracted from the prefrontal cortex (PFC) of rhesus monkeys with TRIzol reagent. mRNA was further purified from total RNA using a purifying kit for mRNA. According to the manual of cDNA Synthesis Kit (200401), ZAP-cDNA Synthesis Kit (200400),and ZAP-cDNA GigapackⅢ Gold Cloning Kit (200450) from Stratagene, we constructed a cDNA-library of the PFC of rhesus monkeys. Our aim was to identify brain-function (such as cognition and memory) related genes and gene families. The cDNA library has a capacity of 2.0×10 6 clones, and 97.3% of them are recombinant clones. The average size of the inserted cDNA fragments is 1.0 kb with the majority of inserts larger than 0.5 kb, which meets the requirement of a standard cDNA library. The PFC cDNA library constructed will be useful in cloning and sequencing brain-function related genes, and in dissecting the alternative splicing patterns of genes expressed in the brain. Meanwhile, it will also serve as a useful resource in understanding the expression regulations within the brain.

LI Yi , , HUANG Wei, ZHANG Xin, SU Bing , *. Construction of a cDNA Library of the Prefrontal Cortex of Rhesus Monkey. Zoological Research, 2006, 27(3): 325-330.
Citation: LI Yi , , HUANG Wei, ZHANG Xin, SU Bing , *. Construction of a cDNA Library of the Prefrontal Cortex of Rhesus Monkey. Zoological Research, 2006, 27(3): 325-330.

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