Volume 14 Issue 3
May  1993
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ZHANG Ya-ping, SHI Li-ming. Mitochondrial DNA Restriction Fragment Length Polymorphism in Presbytis phayrei and P.francoisi. Zoological Research, 1993, 14(3): 263-269.
Citation: ZHANG Ya-ping, SHI Li-ming. Mitochondrial DNA Restriction Fragment Length Polymorphism in Presbytis phayrei and P.francoisi. Zoological Research, 1993, 14(3): 263-269.

Mitochondrial DNA Restriction Fragment Length Polymorphism in Presbytis phayrei and P.francoisi

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1993-08-22
  • Mitochondrial DNAs purified from the livers of P.phayrei and P.francoisi were analyzed with 15 restriction endonucleases.From the 156 bp recognition enzymes used,41-50 sites were observed in the samples.By combining the cleavage patterns for each enzyme,the four samples were classified into four restriction types.The estimated number of nucleotide substitutions per site in P.phayrei,P.francoisi and between the two species was 0.007,0.006 and 0.038,respectively.In combination with our previous data on Macaca mulatta and M.arctoides,the molecular phylogenetic trees of these four species were constructed by the unweighted-pair-group method and the neighbor-joining method.These results provide evidence that intraspecific divergence of P.phayrei and P.francoisi began aout 0.3 Mybp,and that interspecific divergence between these two species and between Colobinae and Cercopithecinae began about 1.9 Mybp and before 11 Mybp,respectively.
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Mitochondrial DNA Restriction Fragment Length Polymorphism in Presbytis phayrei and P.francoisi

Abstract: Mitochondrial DNAs purified from the livers of P.phayrei and P.francoisi were analyzed with 15 restriction endonucleases.From the 156 bp recognition enzymes used,41-50 sites were observed in the samples.By combining the cleavage patterns for each enzyme,the four samples were classified into four restriction types.The estimated number of nucleotide substitutions per site in P.phayrei,P.francoisi and between the two species was 0.007,0.006 and 0.038,respectively.In combination with our previous data on Macaca mulatta and M.arctoides,the molecular phylogenetic trees of these four species were constructed by the unweighted-pair-group method and the neighbor-joining method.These results provide evidence that intraspecific divergence of P.phayrei and P.francoisi began aout 0.3 Mybp,and that interspecific divergence between these two species and between Colobinae and Cercopithecinae began about 1.9 Mybp and before 11 Mybp,respectively.

ZHANG Ya-ping, SHI Li-ming. Mitochondrial DNA Restriction Fragment Length Polymorphism in Presbytis phayrei and P.francoisi. Zoological Research, 1993, 14(3): 263-269.
Citation: ZHANG Ya-ping, SHI Li-ming. Mitochondrial DNA Restriction Fragment Length Polymorphism in Presbytis phayrei and P.francoisi. Zoological Research, 1993, 14(3): 263-269.

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