Volume 17 Issue 2
Mar.  1996
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REN Xiu-hai. Chromosomal Restriction Endonuclease Digestion of A Triploid Grass Cyprinid (Ctenopharyngodon idellus). Zoological Research, 1996, 17(2): 187-192.
Citation: REN Xiu-hai. Chromosomal Restriction Endonuclease Digestion of A Triploid Grass Cyprinid (Ctenopharyngodon idellus). Zoological Research, 1996, 17(2): 187-192.

Chromosomal Restriction Endonuclease Digestion of A Triploid Grass Cyprinid (Ctenopharyngodon idellus)

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1996-05-22
  • In this paper,we report on the cytogenetic detection of a triploid of Ctenophryngodon idellus by restriction endonuclease digestion and conventional banding techniques.Restriction enzymes Hae Ⅲ,Alu Ⅰ (recognizing four base sequences respectively) and Hind Ⅲ (recognizing a six base sequence) induced linear differentiations (G-bands).The isoschizomers Hpa Ⅱ and Map Ⅰ which recognize 4 base sequences resulted in modified C-bands differing from those produced by conventional C-banding.These findings clearly show that restriction endonucleases are capable of producing G-banding patterns in fish chromosomes and that restriction enzyme digestion can be very useful in the analysis of fish chromatin organizatin.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Chromosomal Restriction Endonuclease Digestion of A Triploid Grass Cyprinid (Ctenopharyngodon idellus)

Abstract: In this paper,we report on the cytogenetic detection of a triploid of Ctenophryngodon idellus by restriction endonuclease digestion and conventional banding techniques.Restriction enzymes Hae Ⅲ,Alu Ⅰ (recognizing four base sequences respectively) and Hind Ⅲ (recognizing a six base sequence) induced linear differentiations (G-bands).The isoschizomers Hpa Ⅱ and Map Ⅰ which recognize 4 base sequences resulted in modified C-bands differing from those produced by conventional C-banding.These findings clearly show that restriction endonucleases are capable of producing G-banding patterns in fish chromosomes and that restriction enzyme digestion can be very useful in the analysis of fish chromatin organizatin.

REN Xiu-hai. Chromosomal Restriction Endonuclease Digestion of A Triploid Grass Cyprinid (Ctenopharyngodon idellus). Zoological Research, 1996, 17(2): 187-192.
Citation: REN Xiu-hai. Chromosomal Restriction Endonuclease Digestion of A Triploid Grass Cyprinid (Ctenopharyngodon idellus). Zoological Research, 1996, 17(2): 187-192.

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