Volume 12 Issue 2
Mar.  1991
Turn off MathJax
Article Contents

ZHANG Yun, XIONG Yu-liang. Purification and Characterization of A New Fibrinogenase From The Venom of the Chinese Habu Snake (Trimeresurus mucrosquamatus). Zoological Research, 1991, 12(2): 199-207.
Citation: ZHANG Yun, XIONG Yu-liang. Purification and Characterization of A New Fibrinogenase From The Venom of the Chinese Habu Snake (Trimeresurus mucrosquamatus). Zoological Research, 1991, 12(2): 199-207.

Purification and Characterization of A New Fibrinogenase From The Venom of the Chinese Habu Snake (Trimeresurus mucrosquamatus)

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1991-05-22
  • A new fibrinogenase (EC 3.4.21.5) was isolated and purified from the venom of Chinese habu snake (Trimeresurus mucrosquamatus) by DEAE-SephadexA-50,DEAE-Sepharose CL-6B,MonoQ (FPLC) cclumn chromatography.It showed a single protein band both in sodium dodecyl sulfate (SDS)-polyacrylamide gel egectrophoresis and alkaline polyacrylamide gel electrophoresis.The molecular weight was estimated to be 26000 by SDS-polyacrylamide gel electrophoresis.The isoelectric point was found to be Ph 4.7.It was a glycoprotein containing 6.4% carbohydrate with 0.3% neutral sugar,1.2% sialic acid,4.9% hexosamine.It was composed of about 178 amino acid residues and rich in glycine and aspartic acid.The fibrinogenase of the venom of T.mucrosquamatus TWVFg was heat stable but labile to acid.Its extinction coefficient (1 mg/ml) at 280 nm was 1.558.Purified TMVFg had strong arginine esterase activity;the Km to benzoylarginiue ethylester (BAEE) was 1.4×10[-3]M.The enzyme activity could be inhibited by pheny.Imethanesulfonyfluoride (PMSF);but was not affected by ethylenediamine tetraacetic acid (EDTA).TMVFg had fibrinogenolytic activity;electrophoresis of fibrinogen degraded with TMVFg revealed the rapid disappearance of the α (alpha) and β (beta)-chains and the appearance of lower molecular weight frag ments.TMVFg did not cause fibrinogen solution clotting,nor coagulating plasma and showed neither hemorrhagic activity nor proteolytic activity toward casein.TMVFg had activating fibrinolytic activity.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1812) PDF downloads(1392) Cited by()

Related
Proportional views

Purification and Characterization of A New Fibrinogenase From The Venom of the Chinese Habu Snake (Trimeresurus mucrosquamatus)

Abstract: A new fibrinogenase (EC 3.4.21.5) was isolated and purified from the venom of Chinese habu snake (Trimeresurus mucrosquamatus) by DEAE-SephadexA-50,DEAE-Sepharose CL-6B,MonoQ (FPLC) cclumn chromatography.It showed a single protein band both in sodium dodecyl sulfate (SDS)-polyacrylamide gel egectrophoresis and alkaline polyacrylamide gel electrophoresis.The molecular weight was estimated to be 26000 by SDS-polyacrylamide gel electrophoresis.The isoelectric point was found to be Ph 4.7.It was a glycoprotein containing 6.4% carbohydrate with 0.3% neutral sugar,1.2% sialic acid,4.9% hexosamine.It was composed of about 178 amino acid residues and rich in glycine and aspartic acid.The fibrinogenase of the venom of T.mucrosquamatus TWVFg was heat stable but labile to acid.Its extinction coefficient (1 mg/ml) at 280 nm was 1.558.Purified TMVFg had strong arginine esterase activity;the Km to benzoylarginiue ethylester (BAEE) was 1.4×10[-3]M.The enzyme activity could be inhibited by pheny.Imethanesulfonyfluoride (PMSF);but was not affected by ethylenediamine tetraacetic acid (EDTA).TMVFg had fibrinogenolytic activity;electrophoresis of fibrinogen degraded with TMVFg revealed the rapid disappearance of the α (alpha) and β (beta)-chains and the appearance of lower molecular weight frag ments.TMVFg did not cause fibrinogen solution clotting,nor coagulating plasma and showed neither hemorrhagic activity nor proteolytic activity toward casein.TMVFg had activating fibrinolytic activity.

ZHANG Yun, XIONG Yu-liang. Purification and Characterization of A New Fibrinogenase From The Venom of the Chinese Habu Snake (Trimeresurus mucrosquamatus). Zoological Research, 1991, 12(2): 199-207.
Citation: ZHANG Yun, XIONG Yu-liang. Purification and Characterization of A New Fibrinogenase From The Venom of the Chinese Habu Snake (Trimeresurus mucrosquamatus). Zoological Research, 1991, 12(2): 199-207.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return