Volume 11 Issue 1
Jan.  1990
Turn off MathJax
Article Contents
LIU Nian-kun, XIONG Yu-liang. Purification and Some Properties of the Thrombinlike Enzymes From Trimeresurus Stejnegeri Venom. Zoological Research, 1990, 11(1): 87-93.
Citation: LIU Nian-kun, XIONG Yu-liang. Purification and Some Properties of the Thrombinlike Enzymes From Trimeresurus Stejnegeri Venom. Zoological Research, 1990, 11(1): 87-93.

Purification and Some Properties of the Thrombinlike Enzymes From Trimeresurus Stejnegeri Venom

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1990-02-22
  • Two thrombin-like enzymes (principle Ⅰ and principle Ⅱ) had been purified from the venom of Trimeresurus stejnegeri by CM-Sephadex C-25,DEAE-Sephadex A-50 and DEAE-Sepharose CL-6B column chromatography.Principle Ⅰ showed one band and principle Ⅱ two bands on polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis.We had identified the two bands of principle Ⅱ both were thrombin-like enzymes by the method of cutting polyacrylamide gel electrophoresis.Principle Ⅰ has a molecular weight of 54,500,contains approximately 261 amino acid residues and has a relatively high content of aspartic acid and glutamic acid,contains 14% neutral hexose,13.1% hexosamine and 14.7% sialic acid,the isoelectric point is 3.5,the extinction coefficient at 280 nm for a 0.1% solution is 0.855.Principle Ⅱ has molecular weights of 54,000 and 47,000 respectively as determined by SDS-polyacrylamide gel electrophoresis,principle Ⅱ is also a glycoprotein as showed by Schiffs stain.
  • loading
  • Relative Articles

    [1] Yu-Hong SHI, Rui-Cheng ZHANG, Dan-Yang YANG, Xin-Jiang LU, Jiong CHEN, Min-Yun LI. Yeast-based production, purification and bioactivity assay of rainbow trout LECT2. Zoological Research, 2013, 34(1): 33-38.  doi: 10.3724/SP.J.1141.2013.01033
    [2] yu guo-Yu, XIANG Yang, ZHANG Hong-Yun, JIANG Ping, Lee Wen-Hui, ZHANG Yun, ZHANG. Expression of Bm-TFF2 mutants in Escherichia coli andtheir cell migration-promoting activity. Zoological Research, 2011, 32(4): 379-385.  doi: 10.3724/SP.J.1141.2011.04379
    [3] WANG Yi-Peng, LAI Ren. Insect Antimicrobial Peptides: Structures, Properties and Gene Regulation. Zoological Research, 2010, 31(1): 27-34.  doi: 10.3724/SP.J.1141.2010.01027
    [4] ZHANG Ying-xia, ZOU Ai-hui, MANCHU Ri-ga, ZHOU Yong-can, WANG Shi-feng. Purification and Antimicrobial Activity of Antimicrobial Protein from Brown-spotted Grouper, Epinephelus fario. Zoological Research, 2008, 29(6): 627-632.  doi: 10.3724/SP.J.1141.2008.06627
    [5] CHEN Yao, WANG Shi-quan, HAN Xiao-dong, HOU Ya-yi. Purification and Partial Characterization of Immunoglobulin M from Carassius auratus Serum and Skin Mucus. Zoological Research, 2003, 24(2): 111-115.
    [6] XIAO Yu-cheng, LIANG Song-ping. Purification of Hainantoxin-Ⅴ and Its Characterization of Inhibiting Tetrodotoxin-sensitive Sodium Channel. Zoological Research, 2002, 23(3): 200-205.
    [7] LAI Ren, YE Wen-juan, RAN Yong-lu, LU Shun-qing, YANG Da-tong. Anti-Microbial Peptide From The Skin Secretion of Bombina maxima. Zoological Research, 1998, 19(4): 257-262.
    [8] LIU Yan-shun, WANG Miao, JIN Lei, LI Hong-min, WANG Da-cheng. The Purification and Comparison of Chinese Scorpion Neurotoxins From Different Locations. Zoological Research, 1995, 16(3): 263-270.
    [9] ZHAO Xiao-fan. Purification of A Cysteine Proteinase From Eggs of Silkmoth (Bombyx mori) and Preparation of The Antiserum. Zoological Research, 1993, 14(1): 93-95.
    [10] HE Li-feng, XIAO Chang-hua, DONG Fu-ming, YANG Lian-xi. Studies on The Thrombin-Like Principles of Agkistrodon acutus Venom Ⅲ.Studies on Hemostatic Effect of Hemocoagulase. Zoological Research, 1990, 11(4): 331-335.
    [11] LIU Nian-kun, XIONG Yu-liang. Studies on enzymic Properties of the Thrombin-Like Enzymes From Trimeresurus stejnegeri Venom. Zoological Research, 1990, 11(3): 253-257.
    [12] XIAO Chang-hua, HE Li-feng, TANG Shao-zong, YANG Jun-ru. Studies on the Thrombin-Like Principles of Agkistrodon acutus Venom.Ⅱ.Studies on Activity of Enzymology and Hemotology. Zoological Research, 1989, 10(3): 213-218.
    [13] HU Ren-qiu, WANG Miao, LIU Jian-nin, LEI Ke-jian. Primary Studies of Purification and Partial Characterization of Several New Neurotoxins From East-Asia Scorpion. Zoological Research, 1989, 10(3): 185-193.
    [14] XIAO Chang-hua, HE Li-feng, TAN Shao-zong, YANG Jun-ru, DONG Fu-ming. Studies on the Thrombin-Like Principles of Agkistrodon acutus Venom Ⅰ.Isolation and Purification of its Identification of Physical and Chemical Property. Zoological Research, 1988, 9(zk): 51-59.
    [15] ZHOU Xin-hua. The Isolation,Purification and Some Properties of Two Toxins From the Scorpion Venom of Buthus martensii Karshi From Liaoning. Zoological Research, 1983, 4(2): 201-208.
    [16] RAN Yong-lu et al., LIU Kwang-fen et al., HU Ren-qiu et al., WU Xiang-fu. The Purification and Some Properties of the Phospholipase A From the Venom of Vipera russelli siamensis (From fujian). Zoological Research, 1981, 2(zk): 3-8.
    [17] LEI Ke-jian, HU Ren-qiu, WANG Jin-lan. Purification and Properties of L-Amino Acid Oxidase from Ophiophagus hannah Venom. Zoological Research, 1981, 2(zk): 43-50.
    [18] WU Xiang-fu, CHEN Yuan-chung. Isolation,Purification and Properties of the Basic Phospholipase A From the Snake Venom of Agkistrodon halys Pallas in Zhejiang. Zoological Research, 1981, 2(zk): 13-21.
    [19] SUN Xin, YANG Chang-jiu, CHEN Xi-lan, LAI Ke-jian. Purification and Properties of Four Neurotoxic Fractions From the Venom of Ophiophagvs hannah. Zoological Research, 1981, 2(4): 363-370.
    [20] WANG Chun, CUI Tao, LIU Jin, HUANG Wan-zhi, LU Zi-xian. Purification,Properties and Fluorescence Spectra of the Thrombin-Like Enzyme of Agkistrodon acutus Venom. Zoological Research, 1981, 2(zk): 89-96.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1961) PDF downloads(1579) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return