Volume 1 Issue 1
Jan.  1980
Turn off MathJax
Article Contents

LIU Kwang-fen, CHIOU Shu-yu, LEE Bing, TU Guang-chou. Identification of Physiological Effects and Enzymatic Activities in the Venom of Agkistrodon acutus (From fukien) After Fractionation by Chromatography. Zoological Research, 1980, 1(1): 107-113.
Citation: LIU Kwang-fen, CHIOU Shu-yu, LEE Bing, TU Guang-chou. Identification of Physiological Effects and Enzymatic Activities in the Venom of Agkistrodon acutus (From fukien) After Fractionation by Chromatography. Zoological Research, 1980, 1(1): 107-113.

Identification of Physiological Effects and Enzymatic Activities in the Venom of Agkistrodon acutus (From fukien) After Fractionation by Chromatography

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1980-02-22
  • The venem of Agkistrodon acutus (From Fukien) was fractionated on DEAE-Sephadex A-50 into 11 different protein peaks (Fractions).The experimental conditions are described.In the crude venom,the specific activities of proteinase,arginine esterase,phosphodiesterase,as well as 5-nucleotidase are higher than that of phosphomonoesterase,phospholipase A and L-amino acid oxidase.The arginineesterase and thrombin-like activities are concentrated in fraction Ⅵ.The proteinase,fibrinolytic and anticoagulant activities are concentrated in fraction Ⅰ.Fraction Ⅸ also shows anticoagulant activity.The lethal action is located in fraction Ⅹ.The LD50 after intraperitoneal injectoin of crude venom of Agkistrodon acutus was found to be 4.4 mg/kg for mice.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(119) PDF downloads(0) Cited by()

Related
Proportional views

Identification of Physiological Effects and Enzymatic Activities in the Venom of Agkistrodon acutus (From fukien) After Fractionation by Chromatography

Abstract: The venem of Agkistrodon acutus (From Fukien) was fractionated on DEAE-Sephadex A-50 into 11 different protein peaks (Fractions).The experimental conditions are described.In the crude venom,the specific activities of proteinase,arginine esterase,phosphodiesterase,as well as 5-nucleotidase are higher than that of phosphomonoesterase,phospholipase A and L-amino acid oxidase.The arginineesterase and thrombin-like activities are concentrated in fraction Ⅵ.The proteinase,fibrinolytic and anticoagulant activities are concentrated in fraction Ⅰ.Fraction Ⅸ also shows anticoagulant activity.The lethal action is located in fraction Ⅹ.The LD50 after intraperitoneal injectoin of crude venom of Agkistrodon acutus was found to be 4.4 mg/kg for mice.

LIU Kwang-fen, CHIOU Shu-yu, LEE Bing, TU Guang-chou. Identification of Physiological Effects and Enzymatic Activities in the Venom of Agkistrodon acutus (From fukien) After Fractionation by Chromatography. Zoological Research, 1980, 1(1): 107-113.
Citation: LIU Kwang-fen, CHIOU Shu-yu, LEE Bing, TU Guang-chou. Identification of Physiological Effects and Enzymatic Activities in the Venom of Agkistrodon acutus (From fukien) After Fractionation by Chromatography. Zoological Research, 1980, 1(1): 107-113.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return