Volume 24 Issue 6
Nov.  2003
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FANG Yong-qiang, WENG You-zhu, Liu Li-li, FANG Qi, DAI Yan-yu. Mechanism of 5-HT and GnRH Physiological Action in the Ovary of Crassostrea gigas:Orientation of Double Staining and Immunocytochemistry. Zoological Research, 2003, 24(6): 462-466.
Citation: FANG Yong-qiang, WENG You-zhu, Liu Li-li, FANG Qi, DAI Yan-yu. Mechanism of 5-HT and GnRH Physiological Action in the Ovary of Crassostrea gigas:Orientation of Double Staining and Immunocytochemistry. Zoological Research, 2003, 24(6): 462-466.

Mechanism of 5-HT and GnRH Physiological Action in the Ovary of Crassostrea gigas:Orientation of Double Staining and Immunocytochemistry

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2003-12-22
  • Distribution of 5-HT,GnRH and their receptors within ovaries of Crassostrea gigas was studied using the immunocytochemistry and double staining technique.The results revealed that 1)5-HT and GnRH receptors existed in the oocytes of different development stages of the ovary;2)the reactive substances of 5-HT and GnRH receptors were distributed along the membrane of oocytes and showed dark brown,but nucleus showed immunonegative reaction;3)5-HT,GnRH and their receptors co-existed in the cytoplasm and membrane of oocytes of different development stages.The above results indicate that the oocytes of Crassostrea gigas can not only produce 5-HT and GnRH,but also express their receptors.5-HT and GnRH may exert regulation action on the oocytes by autosecretion.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Mechanism of 5-HT and GnRH Physiological Action in the Ovary of Crassostrea gigas:Orientation of Double Staining and Immunocytochemistry

Abstract: Distribution of 5-HT,GnRH and their receptors within ovaries of Crassostrea gigas was studied using the immunocytochemistry and double staining technique.The results revealed that 1)5-HT and GnRH receptors existed in the oocytes of different development stages of the ovary;2)the reactive substances of 5-HT and GnRH receptors were distributed along the membrane of oocytes and showed dark brown,but nucleus showed immunonegative reaction;3)5-HT,GnRH and their receptors co-existed in the cytoplasm and membrane of oocytes of different development stages.The above results indicate that the oocytes of Crassostrea gigas can not only produce 5-HT and GnRH,but also express their receptors.5-HT and GnRH may exert regulation action on the oocytes by autosecretion.

FANG Yong-qiang, WENG You-zhu, Liu Li-li, FANG Qi, DAI Yan-yu. Mechanism of 5-HT and GnRH Physiological Action in the Ovary of Crassostrea gigas:Orientation of Double Staining and Immunocytochemistry. Zoological Research, 2003, 24(6): 462-466.
Citation: FANG Yong-qiang, WENG You-zhu, Liu Li-li, FANG Qi, DAI Yan-yu. Mechanism of 5-HT and GnRH Physiological Action in the Ovary of Crassostrea gigas:Orientation of Double Staining and Immunocytochemistry. Zoological Research, 2003, 24(6): 462-466.

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