Mechanism of 5-HT and GnRH Physiological Action in the Ovary of Crassostrea gigas:Orientation of Double Staining and Immunocytochemistry
- Received Date: 1900-01-01
- Rev Recd Date: 1900-01-01
- Publish Date: 2003-12-22
Abstract: Distribution of 5-HT,GnRH and their receptors within ovaries of Crassostrea gigas was studied using the immunocytochemistry and double staining technique.The results revealed that 1)5-HT and GnRH receptors existed in the oocytes of different development stages of the ovary;2)the reactive substances of 5-HT and GnRH receptors were distributed along the membrane of oocytes and showed dark brown,but nucleus showed immunonegative reaction;3)5-HT,GnRH and their receptors co-existed in the cytoplasm and membrane of oocytes of different development stages.The above results indicate that the oocytes of Crassostrea gigas can not only produce 5-HT and GnRH,but also express their receptors.5-HT and GnRH may exert regulation action on the oocytes by autosecretion.
|Citation:||FANG Yong-qiang, WENG You-zhu, Liu Li-li, FANG Qi, DAI Yan-yu. Mechanism of 5-HT and GnRH Physiological Action in the Ovary of Crassostrea gigas:Orientation of Double Staining and Immunocytochemistry. Zoological Research, 2003, 24(6): 462-466.|