Volume 34 Issue 2
Mar.  2013
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Cai-Yun WANG, Yun-Han MA, Da-Jian HE, Shi-Hua YANG. cDNA cloning and sequence analysis of pluripotency genes in tree shrews (Tupaia belangeri). Zoological Research, 2013, 34(2): 127-131. doi: 10.3724/SP.J.1141.2013.02127
Citation: Cai-Yun WANG, Yun-Han MA, Da-Jian HE, Shi-Hua YANG. cDNA cloning and sequence analysis of pluripotency genes in tree shrews (Tupaia belangeri). Zoological Research, 2013, 34(2): 127-131. doi: 10.3724/SP.J.1141.2013.02127

cDNA cloning and sequence analysis of pluripotency genes in tree shrews (Tupaia belangeri)

doi: 10.3724/SP.J.1141.2013.02127
  • Received Date: 2012-12-17
  • Rev Recd Date: 2013-01-16
  • Publish Date: 2013-04-08
  • In this paper, partial sequences of the tree shrew (Tupaia belangeri) Klf4, Sox2, and c-Myc genes were cloned and sequenced, which were 382, 612, and 485 bp in length and encoded 127, 204, and 161 amino acids, respectively. Whereas, their cDNA sequence identities with those of human were 89%, 98%, and 89%, respectively. Their phylogenetic tree results indicated different topologies and suggested individual evolutional pathways. These results can facilitate further functional studies.
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    [2] Laga AC, Lai CY, Zhan Q, Huang SJ, Velazquez EF, Yang QH, Hsu MY, Murphy GF. 2010. Expression of the embryonic stem cell transcription factor SOX2 in human skin: relevance to melanocyte and merkel cell biology. The American Journal of Pathology, 176(2): 903-913.
    [3] Okumura-Nakanishi S, Saito M, Niwa H, Ishikawa F. 2005. Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells. The Journal of Biological Chemistry, 280(7): 5307-5317.
    [4] Ping SH, Wang CY, Tang WR, Luo Y, Yang SH. 2012. Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri). Zoological Research, 33(1): 19-28. [平述煌, 王彩云, 唐文如, 罗瑛, 杨世华. 2012. 几种冷冻稀释液与单胺类防冻剂对中缅树鼩精子冷冻存活率的影响. 动物学研究, 33(1): 19-28.]
    [5] Shi Y, Desponts C, Do JT, Hahm HS, Scholer HR, Ding S. 2008. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell, 3(5): 568-574.
    [6] Takahashi K, Yamanaka S. 2006. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 126(4): 663-676.
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    [8] Xu L, Zhang Y, Liang B, Lv LB, Chen CS, Chen YB, Zhou JM, Yao YG. 2013. Tree shrews under the sp ot light: emerging model of human diseases. Zoological Research, 34(2): 59-69. [徐林, 张云, 梁斌, 吕龙宝, 陈策实, 陈勇彬, 周巨民, 姚永刚. 2013. 树鼩实验动物和人类疾病的树鼩模型研究概述. 动物学研究, 34(2): 59-69.]
    [9] Zhang PL, Andrianakos R, Yang Y, Liu CM, Lu WG. 2010. Kruppel-like factor 4 (Klf4) prevents embryonic stem (ES) cell differentiation by regulating Nanog gene expression. Cell, 126(4): 663-676.
    [10] Zhang YX, Ping SH, Yang SH. 2012. Morphological characteristics and cryodamage of Chinese tree shrew (Tupaia belangeri chinensis) sperm. Zoologcial Research, 33(1): 29-36. [张远旭, 平述煌, 杨世华. 2012. 中缅树鼩精子形态特征及冷冻损伤. 动物学研究, 33(1): 29-36.]
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cDNA cloning and sequence analysis of pluripotency genes in tree shrews (Tupaia belangeri)

doi: 10.3724/SP.J.1141.2013.02127

Abstract: In this paper, partial sequences of the tree shrew (Tupaia belangeri) Klf4, Sox2, and c-Myc genes were cloned and sequenced, which were 382, 612, and 485 bp in length and encoded 127, 204, and 161 amino acids, respectively. Whereas, their cDNA sequence identities with those of human were 89%, 98%, and 89%, respectively. Their phylogenetic tree results indicated different topologies and suggested individual evolutional pathways. These results can facilitate further functional studies.

Cai-Yun WANG, Yun-Han MA, Da-Jian HE, Shi-Hua YANG. cDNA cloning and sequence analysis of pluripotency genes in tree shrews (Tupaia belangeri). Zoological Research, 2013, 34(2): 127-131. doi: 10.3724/SP.J.1141.2013.02127
Citation: Cai-Yun WANG, Yun-Han MA, Da-Jian HE, Shi-Hua YANG. cDNA cloning and sequence analysis of pluripotency genes in tree shrews (Tupaia belangeri). Zoological Research, 2013, 34(2): 127-131. doi: 10.3724/SP.J.1141.2013.02127
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