Jinling CAO, Jianjie CHEN, Zhongliang JIANG, Yongju LUO, Xi GAN. 2012: Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus. Zoological Research, 33(E3-4): 40-46. DOI: 10.3724/SP.J.1141.2012.E03-04E40
Citation: Jinling CAO, Jianjie CHEN, Zhongliang JIANG, Yongju LUO, Xi GAN. 2012: Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus. Zoological Research, 33(E3-4): 40-46. DOI: 10.3724/SP.J.1141.2012.E03-04E40

Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus

  • The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination. We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia (gfFTZ-F1). The full-length cDNA was isolated from the ovary, which included an open reading frame encoding a predicted protein of 486 amino acids. Sequence, tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues. Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts, though further study is needed to make any definitive conclusions.
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