Jinling CAO, Jianjie CHEN, Zhongliang JIANG, Yongju LUO, Xi GAN. Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus. Zoological Research, 2012, 33(E3-4): 40-46. doi: 10.3724/SP.J.1141.2012.E03-04E40
Citation: Jinling CAO, Jianjie CHEN, Zhongliang JIANG, Yongju LUO, Xi GAN. Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus. Zoological Research, 2012, 33(E3-4): 40-46. doi: 10.3724/SP.J.1141.2012.E03-04E40

Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus

doi: 10.3724/SP.J.1141.2012.E03-04E40
Funds:  the Natural Science Foundation of Shanxi Province, China (2009021038); the Doctor Initial Foundation of Shanxi Agricultural University (XB2009003); the Science and Technology Innovational Foundation of Shanxi Agricultural University (2009005); the Nonprofit Research Institutes, Special Foundation for Operating Expenses of Basic Research Projects of Guangxi Zhuang Autonomous Region(2060302 GXIF-2008-01); the Scientific Research and Technological Development Projects of Nanning City(200801016B)
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  • Author Bio:

    Jinling CAO

  • Corresponding author: Jinling CAO
  • Received Date: 2012-02-27
  • Rev Recd Date: 2012-06-01
  • Publish Date: 2012-08-10
  • The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination. We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia (gfFTZ-F1). The full-length cDNA was isolated from the ovary, which included an open reading frame encoding a predicted protein of 486 amino acids. Sequence, tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues. Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts, though further study is needed to make any definitive conclusions.
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Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus

doi: 10.3724/SP.J.1141.2012.E03-04E40
Funds:  the Natural Science Foundation of Shanxi Province, China (2009021038); the Doctor Initial Foundation of Shanxi Agricultural University (XB2009003); the Science and Technology Innovational Foundation of Shanxi Agricultural University (2009005); the Nonprofit Research Institutes, Special Foundation for Operating Expenses of Basic Research Projects of Guangxi Zhuang Autonomous Region(2060302 GXIF-2008-01); the Scientific Research and Technological Development Projects of Nanning City(200801016B)
  • Author Bio:

  • Corresponding author: Jinling CAO

Abstract: The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination. We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia (gfFTZ-F1). The full-length cDNA was isolated from the ovary, which included an open reading frame encoding a predicted protein of 486 amino acids. Sequence, tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues. Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts, though further study is needed to make any definitive conclusions.

Jinling CAO, Jianjie CHEN, Zhongliang JIANG, Yongju LUO, Xi GAN. Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus. Zoological Research, 2012, 33(E3-4): 40-46. doi: 10.3724/SP.J.1141.2012.E03-04E40
Citation: Jinling CAO, Jianjie CHEN, Zhongliang JIANG, Yongju LUO, Xi GAN. Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus. Zoological Research, 2012, 33(E3-4): 40-46. doi: 10.3724/SP.J.1141.2012.E03-04E40
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