Zhiying JIA, Yuyong ZHANG, Shuqiang CHEN, Lianyu SHI. Genetic diversity and differentiation of masu salmon (Oncorhynchus masou masou) between and within cultured populations inferred from microsatellite DNA analysis. Zoological Research, 2012, 33(E3-4): 33-39. doi: 10.3724/SP.J.1141.2012.E03-04E33
Citation: Zhiying JIA, Yuyong ZHANG, Shuqiang CHEN, Lianyu SHI. Genetic diversity and differentiation of masu salmon (Oncorhynchus masou masou) between and within cultured populations inferred from microsatellite DNA analysis. Zoological Research, 2012, 33(E3-4): 33-39. doi: 10.3724/SP.J.1141.2012.E03-04E33

Genetic diversity and differentiation of masu salmon (Oncorhynchus masou masou) between and within cultured populations inferred from microsatellite DNA analysis

doi: 10.3724/SP.J.1141.2012.E03-04E33
Funds:  HRFRI Basic Science Research Special Funds (2009HSYZX-YY-10); Heilongjiang Natural Science Fund (C201044); Heilongjiang Postdoctoral Sustentation Fund (LRB10-081)
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  • Author Bio:

    Zhiying JIA1

  • Corresponding author: Lianyu SHI
  • Received Date: 2011-12-08
  • Rev Recd Date: 2012-06-04
  • Publish Date: 2012-08-10
  • Masu salmon, Oncorhynchus masou masou, is one of the most valuable fishery species that has been introduced to China, though to date no studies on the genetic diversity and genetic relationship among hatchery populations has been performed with molecular markers. We undertook such a study and sampled 120 individuals from three hatchery stocks and analyzed 20 microsatellite loci. All loci were polymorphic and a total of 91 alleles were detected. A relatively low level of genetic diversity was revealed with effective number of allele of 3.1094, 3.3299 and 3.1894 and expected heterozygosity of 0.6600, 0.6648 and 0.6638 in the three stocks, respectively. Deviations from Hardy-Weinberg equilibrium were found due to heterozygote deficit. Accordingly, evidence of genetic bottlenecks were found in the three stocks. An individual assignment test demonstrated that 85% of individuals were correctly assigned into their original stocks. Pairwise Fst revealed that significant differentiation occurred between these three stocks. The results of the study indicated that disequilibrium of genetic structure and differentiation has occurred in all three stocks. This information collectively provides a basis for measures to avoid of loss of genetic diversity and introgression in Chinese aquaculture.
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  • [1] Aung O, Nguyen TTT, Poompuang S, Kamonrat W. 2010. Microsatellite DNA markers revealed genetic population structure among captive stocks and wild populations of mrigal, Cirrhinus cirrhosus in Myanmar [J]. Aquaculture, 299(1-4): 37-43.
    [2] Cornuet JM, Luikart G. 1997. Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data [J]. Genetics, 144(4): 2001-2014.
    [3] Cornuet JM, Piry S, Luikart G, Estoup A, Solignac M. 1999. New methods employing multilocus genotypes to select or exclude populations as origins of individuals [J]. Genetics, 153(4): 1989-2000.
    [4] De Woody JA, Avise JC. 2000. Microsatellite variation in marine, freshwater and anadromous fishes compared with other animals [J]. J Fish Biol, 56(3): 461-473.
    [5] Evanno G, Regnaut S, Goudet J. 2005. Detecting the number of clusters of individuals using the software structure: a simulation study[J]. Mol Ecol, 14(8), 2611-2620.
    [6] Felsenstein J. 1993. PHYLIP (phylogeny inference package), version 3.6 [C] // Department of Genome Sciences and Department of Biology. Seattle, USA: University of Washington.
    [7] Filgueira R, Grant J, Strand Ø, Asplinc L, Aurec J. 2010. A simulation model of carrying capacity for mussel culture in a Norwegian fjord: role of induced upwelling [J]. Aquaculture, 308(1-2): 20-27.
    [8] Jia ZY, Zhang YY, Shi LL, Bai QL, Jin SB, Mou ZB. 2008. Amplification of rainbow trout microsatellites in Brachymystax lenok [J]. Mol Ecol Resour, 8(6): 1520-1521.
    [9] Kano Y, Kondou T, Shimizu Y. 2010. Present status and conservation of the markless forms of stream-resident masu salmon Oncorhynchus masou (the so-called ‘iwame’) in Japanese mountain streams [J]. Ichthyol Res, 57(1): 78-84.
    [10] Kohlmann K, Kersten P, Flajšhans M. 2005. Microsatellite-based genetic variability and differentiation of domesticated, wild and feral common carp (Cyprinus caprio L.) populations [J]. Aquaculture, 247(1-4): 253-266.
    [11] Na-Nakorn U, Kamonrat W, Ngamsiri T. 2004. Genetic diversity of walking catfish, Clarias macrocephalus, in Thailand and evidence of genetic introgression from introduced farmed C. gariepinus [J]. Aquaculture, 240(1-4): 145-163.
    [12] Norris AT, Bradley DG, Cunningham EP. 1999. Microsatellite genetic variation between and within farmed and wild Atlantic salmon (Salmo salar) populations [J]. Aquaculture, 180(3-4): 247-264.
    [13] Pritchard JK, Stephens M, Donnelly P. 2000. Inference of population structure using multilocus genotype data [J]. Genetics, 155(2): 945-959.
    [14] Rexroad CE III, Coleman RL, Gustafson AL, Hershberger WK, Killefer J. 2002a. Development of rainbow trout microsatellite markers from repeat enriched libraries [J]. Mar Biotechnol, 4(1): 12-16.
    [15] Rexroad CE III, Coleman RL, Hershberger WK, Killefer J. 2002b. Rapid communication: thirty-eight polymorphic microsatellite markers for mapping in rainbow trout [J]. J Anim Sci, 80(2): 541-542.
    [16] Rexroad CE III, Rodriguez MF, Coulibaly I, Gharbi K, Danzmann RG, Dekoning J, Phillips R, Palti Y. 2005. Comparative mapping of expressed sequence tags containing microsatellites in rainbow trout (Oncorhynchus mykiss) [J]. BMC Genomics, 6: 54.
    [17] Raymond M, Rousset F. 1995. GENEPOP (version 1.2): population genetic software for exact tests and ecumenicism [J]. J Hered, 86(3): 248-249.
    [18] Sekino M, Hara M, Taniguchi N. 2002. Loss of microsatellite and mitochondrial DNA variation in hatchery strains of Japanese flounder Paralichthys olivaceus [J]. Aquaculture, 213(1-4): 101-122.
    [19] Sokal RR, Rohlf FJ. 1995. Biometry, 3rd ed. [M]. New York: W.H. Freeman and Company.
    [20] Wang ZM. 1998. Masu salmon-A new cold-water cultured fish [J]. Chn J Fish, 11(2): 96. (in Chinese)
    [21] Wei DW, Lou YD, Sun XW, Shen JB. 2001. Isolation of microsatellite markers in the common carp (Cyprinus carpio) [J]. Zool Res, 22(3): 238-241. (in Chinese)
    [22] Yeh FC, Yang RC,  Boyle TBJ. 1999. POPGENE, version 1.32, Microsoft Window-Based software for Population Genetic Analysis: a quick user’s guide[M]. University of Alberta, Edmonton, Canada.
    [23] Yu JN, Azuma N, Yoon M, Brykov V, Urawa S, Nagat M, Jin DH, Abe S. 2010. Population genetic structure and phylogeography of masu salmon (Oncorhynchus masou masou) inferred from mitochondrial and microsatellite DNA analyses [J]. Zool Sci, 27(5): 375-385.
    [24] Zhang YY, Jia ZY, Bai QL, Tang YK. 2011. Properties of silvering salmon in Oncorhynchus masou masou population under aquaculture condition [J]. Chn J Zool, 46(4): 8-15. (in Chinese)
    [25]
    [26]  
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Genetic diversity and differentiation of masu salmon (Oncorhynchus masou masou) between and within cultured populations inferred from microsatellite DNA analysis

doi: 10.3724/SP.J.1141.2012.E03-04E33
Funds:  HRFRI Basic Science Research Special Funds (2009HSYZX-YY-10); Heilongjiang Natural Science Fund (C201044); Heilongjiang Postdoctoral Sustentation Fund (LRB10-081)
  • Author Bio:

  • Corresponding author: Lianyu SHI

Abstract: Masu salmon, Oncorhynchus masou masou, is one of the most valuable fishery species that has been introduced to China, though to date no studies on the genetic diversity and genetic relationship among hatchery populations has been performed with molecular markers. We undertook such a study and sampled 120 individuals from three hatchery stocks and analyzed 20 microsatellite loci. All loci were polymorphic and a total of 91 alleles were detected. A relatively low level of genetic diversity was revealed with effective number of allele of 3.1094, 3.3299 and 3.1894 and expected heterozygosity of 0.6600, 0.6648 and 0.6638 in the three stocks, respectively. Deviations from Hardy-Weinberg equilibrium were found due to heterozygote deficit. Accordingly, evidence of genetic bottlenecks were found in the three stocks. An individual assignment test demonstrated that 85% of individuals were correctly assigned into their original stocks. Pairwise Fst revealed that significant differentiation occurred between these three stocks. The results of the study indicated that disequilibrium of genetic structure and differentiation has occurred in all three stocks. This information collectively provides a basis for measures to avoid of loss of genetic diversity and introgression in Chinese aquaculture.

Zhiying JIA, Yuyong ZHANG, Shuqiang CHEN, Lianyu SHI. Genetic diversity and differentiation of masu salmon (Oncorhynchus masou masou) between and within cultured populations inferred from microsatellite DNA analysis. Zoological Research, 2012, 33(E3-4): 33-39. doi: 10.3724/SP.J.1141.2012.E03-04E33
Citation: Zhiying JIA, Yuyong ZHANG, Shuqiang CHEN, Lianyu SHI. Genetic diversity and differentiation of masu salmon (Oncorhynchus masou masou) between and within cultured populations inferred from microsatellite DNA analysis. Zoological Research, 2012, 33(E3-4): 33-39. doi: 10.3724/SP.J.1141.2012.E03-04E33
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