Volume 33 Issue 3
May  2012
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SHI Mei-Lian, XU Ping, YIN Xiao-Shu, YANG Wei-Wei, GU Mei-Er, YU LI-Ping, LIU Gui-Jie, WU Bao-Jin. Phenotype analysis and mutant gene location of ventral yellow mouse (VYSlac). Zoological Research, 2012, 33(3): 290-297. doi: 10.3724/SP.J.1141.2012.03290
Citation: SHI Mei-Lian, XU Ping, YIN Xiao-Shu, YANG Wei-Wei, GU Mei-Er, YU LI-Ping, LIU Gui-Jie, WU Bao-Jin. Phenotype analysis and mutant gene location of ventral yellow mouse (VYSlac). Zoological Research, 2012, 33(3): 290-297. doi: 10.3724/SP.J.1141.2012.03290

Phenotype analysis and mutant gene location of ventral yellow mouse (VYSlac)

doi: 10.3724/SP.J.1141.2012.03290
  • Received Date: 2011-11-21
  • Rev Recd Date: 2012-04-09
  • Publish Date: 2012-06-22
  • The ventri-yellow pigmentation mouse (temporarily named VYSlac) arose spontaneously in the C57BL/6J inbred mouse strain, found and bred by Shanghai SLAC Laboratory Animal Co., Ltd. VYSlac presented a special phenotype marked by yellow coat on the ventral surface of neck and trunk that was without melanin deposition but maintained a normal structure. The number of melanocytes in epidermis and melanin in hair follicle of the abdominal skin of the mutant mouse were less than that of their background strain, while there was no significant difference between the dorsal skins of the two strains. This mutant phenotype was inherited as single-gene dominant inheritance, confirmed by genetic experiment, and there was no significant difference between VYSlac and B6 for other biological parameters such as weight, anatomic and histological structures of major organs and blood physiology. When the linkage relationship between the genomic DNA samples of F2 48 mice (VYSlacD2F12) and mutant phenotype were evaluated, the mutant gene was confirmed on chromosome 2 near D2Mit229. New microsatellite and SNP markers were selected to amplify genomic DNA samples of 196 F2 mice and the mutant gene was narrowed down to 5.3 Mb region between rs13476833 and rs27310903 on chromosome 2. The preliminary results of our phenotype analysis and gene location provides a solid basis for further identification of this mutant gene.
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  • [1]
    Balling R. 2001. ENU mutagenesis: analyzing gene function in mice [J].Annu Rev Genomics Hum Genet, 2: 463-492.
    Caspary T. 2010. Phenotype-driven mouse ENU mutagenesis screens [J].Methods Enzymol, 477: 313-327.
    Gong ZJ, Zhan RZ. 1994. Pathological Production and Staining Techniques [M]. Shanghai: Shanghai Scientific and Technical Publishing House. [龚志锦, 詹镕洲. 1994. 病理组织制片和染色技术 [M]. 上海: 上 海科学技术出版社.]
    Hornyak TJ. 2006. The developmental biology of melanocytes and its application to understanding human congenital disorders of pigmentation [J]. Adv Dermatol, 22: 201-218.
    Liu Y, Nie WH, Huang L, Wang JH, Su WT, Lin CC,Yang FT. 2008.Cloning, characterization, and FISH mapping of four satellite DNAs from black muntjac (Muntiacus crinifrons) and Fea’s muntjac (M. feae) [J]. Zool Res,29 (3): 225-235. [刘 妍,佴文惠,黄 玲,王金焕,苏 伟婷, LIN Chyi Chyang,杨凤堂. 2008.黑麂和费氏麂四种卫星DNA 的克隆特征和染色体定位[J]. 动物学研究,29 (3): 225-235.]
    Manceau M, Domingues VS, Mallarino R, Hoekstra HE. 2011. The developmental role of Agouti in color pattern evolution [J]. Science, 331(6020): 1062-1065.
    Matsunaga N, Virador V, Santis C, Vieira WD, Furumura M, Matsunaga J,Kobayashi N, Hearing VJ. 2000. In situ localization of agouti signal protein in murine skin using immunohistochemistry with an ASP-specific antibody [J]. Biochem Biophys Res Commun, 270(1): 176-182.
    Morgan H, Beck T, Blake A, Gates H, Adams N, Debouzy G, Leblanc S,Lengger C, Maier H, Melvin D, Meziane H, Richardson D, Wells S, White J, Wood J, EUMODIC Consortium, de Angelis MH, Brown SDM, Hancock JM, Mallon AM. 2010. EuroPhenome: a repository for high-throughput mouse phenotyping data [J]. Nucleic Acids Res, 38(S1): D577-D585.
    Overton JD, Leibel RL. 2011. Mahoganoid and mahogany mutations rectify the obesity of the yellow mouse by effects on endosomal traffic of MC4R protein [J]. J Biol Chem, 286(21): 18914-18929.
    Price ER, Fischer DE. 2001. Sensorineural deafness and pigmentation genes: melanocytes and the Mitf transcriptional network [J]. Neuron, 30(1): 15-18.
    Scattoni ML, Gandhy SU, Ricceri L, Crawley JN. 2008. Unusual repertoire of vocalizations in the BTBR T+tf/J mouse model of autism [J]. PLoS One, 3(8): e3067.
    Spritz RA, Chiang PW, Oiso N, Alkhateeb A. 2003. Human and mouse disorders of pigmentation [J]. Curr Opin Genet Dev, 13(3): 284-289.
    Stottmann RW, Beier DR. 2010. Using ENU mutagenesis for phenotype-driven analysis of the mouse[J]. Methods Enzymol, 477: 329-348.
    Wahlsten D, Metten P, Crabbe JC. 2003. Survey of 21 inbred mouse strains in two laboratories reveals that BTBR T/+ tf/tf has severely reduced hippocampal commissure and absent corpus callosum [J]. Brain Res, 971(1): 47-54.
    Wilkinson P, Sengerova J, Matteoni R, Chen CK, Soulat G, Ureta-Vidal A,Fessele S, Hagn M, Massimi M, Pickford K, Butler RH, Marschall S,Mallon AM, Pickard A, Raspa M, Scavizzi F, Fray M, Larrigaldie V,Leyritz J, Birney E, Tocchini-Valentini GP, Brown S, Herault Y,Montoliu L, de Angelis MH, Smedley D. 2010. EMMA-mouse mutant resources for the international scientific community [J]. Nucleic Acids Res, 38: D570-D576.
    Wu BJ, Mao HH, Shao YX, Xue ZF, Li HD. 2003a. Four kinds of ENU-induced white spot mice and chromosome locations of the mutant genes [J]. Chin Sci Bull, 48(24): 2658-2664.
    Wu BJ, Mao HH, Zhu H, Yan ZF, Yang L, Sun Q, Xu XM, Xue ZF, Li HD. 2003b. PCR conditions and application of 39 mouse microsatellites [J].Acta Lab Anim Sci Sin, 11(4): 216-220. [吴宝金, 茅慧华, 朱洪, 阎志 峰, 杨玲, 孙强, 徐向明, 薛整风, 李厚达. 2003. 小鼠39 个微卫星 的PCR 条件及其运用 [J]. 中国实验动物学报, 11(4): 216-220.]
    Wu BJ, Yin LJ, Lu ZL, Yin YS, Yang WW, Yang R, Kang XD, Liu GJ, Yin HP, Yu LP, Gu ME, Wu PL. 2010. Abnormal gonad development in KitW-2Bao mice caused by a Kit gene missense mutation [J]. Chin Sci Bull, 55(36): 4143-4149.
    Wu PL, Yin HP, Yin LJ, Zhu J, Zeng YM, Liu GJ, Kang XD, Yu LP, Gu ME,Yuan H, Wu BJ. 2009. Gonadial abnormality and homozygous decease from the nonsense mutation of Kit in W3Bao Mouse [J]. Zool Res, 30(1): 45-52. [吴培林, 尹洪萍, 殷黎静, 朱洁, 曾咏梅, 刘桂杰, 亢晓冬, 俞利平, 顾美儿, 袁红, 吴宝金. 2009. Kit 无义突变致W3Bao小鼠生 殖腺异常及纯合子贫血死亡[J]. 动物学研究, 30(1): 45-52.]
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