Volume 33 Issue 2
Mar.  2012
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SONG Hai-Yan, LIU Zai-Qun, ZHENG Lei. Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose. Zoological Research, 2012, (2): 211-217. doi: 10.3724/SP.J.1141.2012.02211
Citation: SONG Hai-Yan, LIU Zai-Qun, ZHENG Lei. Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose. Zoological Research, 2012, (2): 211-217. doi: 10.3724/SP.J.1141.2012.02211

Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose

doi: 10.3724/SP.J.1141.2012.02211
  • Received Date: 2011-12-01
  • Rev Recd Date: 2012-01-09
  • Publish Date: 2012-04-22
  • The strep avidin-biotin-peroxidase complex (SABC) immunohistochemical methods were applied to investigate the localization and semi-quantitative distribution of IFN-γ, IL-1α, NGF-β and TNF-α-immunoreactive cells in the cerebellar cortex of Western Anhui white goose at embryonic day 13, 19, 24, 28 (E13, E19, E24, E28) and postnatal day 7, 15 (P7, P15). The possible roles of IFN-γ、IL-1α、NGF-β and TNF-α in the development of cerebellar cortex were discussed. The results indicated that in the external granular layer, there were IFN-γ and TNF-αpositive cells at E13, E19, E24, E28, P7, IL-1α positive cells at E13, E19, E24, E28 and NGF-β positive cells at E13,E19, E24. The expression levels of these four cytokines all reached peaks at E19 of the six tested periods in this study. In the Purkinje cell layer, there were IFN-γ, IL-1α and TNF-α positive cells at E13, E19, E24, E28, P7, P15 and NGF-βpositive cells at E13, E19, E24, E28, P7. In the internal granular layer, there were IFN-γ positive cells at E13, E19, E24,E28, P7, P15, IL-1α and TNF-α positive cells at E13, E19, E24, E28, P7 and NGF-β positive cells at E13, E19, E24, E28.These results showed that E19 might be the “critical stage” in the cerebellar cortex development of Western Anhui white goose. IFN-γ, IL-1α and TNF-α might be synthesized by cerebellar cortex itself, and NGF-β could be transported from regions which project to Purkinje cells. IFN-γ may interfer the transfer of granular cells, and NGF-β may have neurotrophic functions that are beneficial to the growth and development of Purkinje cells.
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Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose

doi: 10.3724/SP.J.1141.2012.02211

Abstract: The strep avidin-biotin-peroxidase complex (SABC) immunohistochemical methods were applied to investigate the localization and semi-quantitative distribution of IFN-γ, IL-1α, NGF-β and TNF-α-immunoreactive cells in the cerebellar cortex of Western Anhui white goose at embryonic day 13, 19, 24, 28 (E13, E19, E24, E28) and postnatal day 7, 15 (P7, P15). The possible roles of IFN-γ、IL-1α、NGF-β and TNF-α in the development of cerebellar cortex were discussed. The results indicated that in the external granular layer, there were IFN-γ and TNF-αpositive cells at E13, E19, E24, E28, P7, IL-1α positive cells at E13, E19, E24, E28 and NGF-β positive cells at E13,E19, E24. The expression levels of these four cytokines all reached peaks at E19 of the six tested periods in this study. In the Purkinje cell layer, there were IFN-γ, IL-1α and TNF-α positive cells at E13, E19, E24, E28, P7, P15 and NGF-βpositive cells at E13, E19, E24, E28, P7. In the internal granular layer, there were IFN-γ positive cells at E13, E19, E24,E28, P7, P15, IL-1α and TNF-α positive cells at E13, E19, E24, E28, P7 and NGF-β positive cells at E13, E19, E24, E28.These results showed that E19 might be the “critical stage” in the cerebellar cortex development of Western Anhui white goose. IFN-γ, IL-1α and TNF-α might be synthesized by cerebellar cortex itself, and NGF-β could be transported from regions which project to Purkinje cells. IFN-γ may interfer the transfer of granular cells, and NGF-β may have neurotrophic functions that are beneficial to the growth and development of Purkinje cells.

SONG Hai-Yan, LIU Zai-Qun, ZHENG Lei. Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose. Zoological Research, 2012, (2): 211-217. doi: 10.3724/SP.J.1141.2012.02211
Citation: SONG Hai-Yan, LIU Zai-Qun, ZHENG Lei. Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose. Zoological Research, 2012, (2): 211-217. doi: 10.3724/SP.J.1141.2012.02211
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