Volume 33 Issue 2
Mar.  2012
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CHEN Mei, TIAN Li-Li, SHI Qing-Hui, CAO Tian-Wen, HAO Jia-Sheng. Complete mitogenome of the Lesser Purple Emperor Apatura ilia (Lepidoptera: Nymphalidae: Apaturinae) and comparison with other nymphalid butterflies. Zoological Research, 2012, (2): 191-201. doi: 10.3724/SP.J.1141.2012.02191
Citation: CHEN Mei, TIAN Li-Li, SHI Qing-Hui, CAO Tian-Wen, HAO Jia-Sheng. Complete mitogenome of the Lesser Purple Emperor Apatura ilia (Lepidoptera: Nymphalidae: Apaturinae) and comparison with other nymphalid butterflies. Zoological Research, 2012, (2): 191-201. doi: 10.3724/SP.J.1141.2012.02191

Complete mitogenome of the Lesser Purple Emperor Apatura ilia (Lepidoptera: Nymphalidae: Apaturinae) and comparison with other nymphalid butterflies

doi: 10.3724/SP.J.1141.2012.02191
Funds:  This work was supported by grants from the National Science Foundation of China (41172004, 40871034) and partially by the Provincial Key Projects of Natural Science Foundation, Colleges of Anhui Province (KJ2010A142)
  • Received Date: 2011-10-24
  • Rev Recd Date: 2012-02-20
  • Publish Date: 2012-04-22
  • The complete mitochondrial genome of Apatura ilia (GenBank accession no. JF437925) was determined as a circular DNA molecule of 15 242 bp, with common genes of 13 putative proteins, 2 rRNAs, and 22 tRNAs and of the same gene arrangement as in other sequenced lepidopterans. All protein-coding genes had the typical start codon ATN,except for the COI’s using CGA as its start codon as previously demonstrated in other lepidopteran species. The comparison of the nucleotide sequences of the A. ilia mitogenome with ten other Nymphalidae species showed nearly identical gene orientation and arrangement, with only a few alterations in non-coding fragments. The nucleotide composition and codon frequency all fell into the range estimated for the order Lepidoptera. The A. ilia mitochondrial genome had the canonical set of 22 tRNA genes folded in the typical cloverleaf structure, with an unique exception of tRNASer(AGN). The mitochondrial genes from A. ilia were overlapped in a total of 33 bp at 9 locations, as well as interleaved with a total of 155 bp intergenic spacers, spread over 12 regions with the size ranging from 1 to 49 bp.Furthermore, the spacer between ND6 and Cyt b harbored a microsatellite-like repeat (TA)23 not found in other completely sequenced nymphalid genomes. The 403 bp AT-rich region harbored two conserved motifs (ATAGA,ATTTA), a 21 bp polyT stretch, a 10 bp poly-A region, along with two microsatellite-like repeats ((TA)10and (TA)7), as detected in other nymphalid butterflies
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Complete mitogenome of the Lesser Purple Emperor Apatura ilia (Lepidoptera: Nymphalidae: Apaturinae) and comparison with other nymphalid butterflies

doi: 10.3724/SP.J.1141.2012.02191
Funds:  This work was supported by grants from the National Science Foundation of China (41172004, 40871034) and partially by the Provincial Key Projects of Natural Science Foundation, Colleges of Anhui Province (KJ2010A142)

Abstract: The complete mitochondrial genome of Apatura ilia (GenBank accession no. JF437925) was determined as a circular DNA molecule of 15 242 bp, with common genes of 13 putative proteins, 2 rRNAs, and 22 tRNAs and of the same gene arrangement as in other sequenced lepidopterans. All protein-coding genes had the typical start codon ATN,except for the COI’s using CGA as its start codon as previously demonstrated in other lepidopteran species. The comparison of the nucleotide sequences of the A. ilia mitogenome with ten other Nymphalidae species showed nearly identical gene orientation and arrangement, with only a few alterations in non-coding fragments. The nucleotide composition and codon frequency all fell into the range estimated for the order Lepidoptera. The A. ilia mitochondrial genome had the canonical set of 22 tRNA genes folded in the typical cloverleaf structure, with an unique exception of tRNASer(AGN). The mitochondrial genes from A. ilia were overlapped in a total of 33 bp at 9 locations, as well as interleaved with a total of 155 bp intergenic spacers, spread over 12 regions with the size ranging from 1 to 49 bp.Furthermore, the spacer between ND6 and Cyt b harbored a microsatellite-like repeat (TA)23 not found in other completely sequenced nymphalid genomes. The 403 bp AT-rich region harbored two conserved motifs (ATAGA,ATTTA), a 21 bp polyT stretch, a 10 bp poly-A region, along with two microsatellite-like repeats ((TA)10and (TA)7), as detected in other nymphalid butterflies

CHEN Mei, TIAN Li-Li, SHI Qing-Hui, CAO Tian-Wen, HAO Jia-Sheng. Complete mitogenome of the Lesser Purple Emperor Apatura ilia (Lepidoptera: Nymphalidae: Apaturinae) and comparison with other nymphalid butterflies. Zoological Research, 2012, (2): 191-201. doi: 10.3724/SP.J.1141.2012.02191
Citation: CHEN Mei, TIAN Li-Li, SHI Qing-Hui, CAO Tian-Wen, HAO Jia-Sheng. Complete mitogenome of the Lesser Purple Emperor Apatura ilia (Lepidoptera: Nymphalidae: Apaturinae) and comparison with other nymphalid butterflies. Zoological Research, 2012, (2): 191-201. doi: 10.3724/SP.J.1141.2012.02191
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