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TIAN Wei-Wei, GAO Yue-Dong, GUO Yan, HUANG Jing-Fei, XIAO Chang, LI Zuo-Sheng, ZHANG Hua-Tang. Cloning of full-length coding sequence of tree shrew CD4 and prediction of its molecular characteristics. Zoological Research, 2012, 33(1): 60-66. doi: 10.3724/SP.J.1141.2012.01060
Citation: TIAN Wei-Wei, GAO Yue-Dong, GUO Yan, HUANG Jing-Fei, XIAO Chang, LI Zuo-Sheng, ZHANG Hua-Tang. Cloning of full-length coding sequence of tree shrew CD4 and prediction of its molecular characteristics. Zoological Research, 2012, 33(1): 60-66. doi: 10.3724/SP.J.1141.2012.01060

Cloning of full-length coding sequence of tree shrew CD4 and prediction of its molecular characteristics

doi: 10.3724/SP.J.1141.2012.01060
  • Received Date: 2011-10-09
  • Rev Recd Date: 2012-01-05
  • Publish Date: 2012-02-22
  • The tree shrews, as an ideal animal model receiving extensive attentions to human disease research, demands essential research tools, in particular cellular markers and monoclonal antibodies for immunological studies. In this paper, a 1 365 bp of the full-length CD4 cDNA encoding sequence was cloned from total RNA in peripheral blood of tree shrews, the sequence completes two unknown fragment gaps of tree shrews predicted CD4 cDNA in the GenBank database, and its molecular characteristics were analyzed compared with other mammals by using biology software such as Clustal W2.0 and so forth. The results showed that the extracellular and intracellular domains of tree shrews CD4 amino acid sequence are conserved. The tree shrews CD4 amino acid sequence showed a close genetic relationship with Homo sapiens and Macaca mulatta. Most regions of the tree shrews CD4 molecule surface showed positive charges as humans. However, compared with CD4 extracellular domain D1 of human, CD4 D1 surface of tree shrews showed more negative charges, and more two N-glycosylation sites, which may affect antibody binding. This study provides a theoretical basis for the preparation and functional studies of CD4 monoclonal antibody.
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Cloning of full-length coding sequence of tree shrew CD4 and prediction of its molecular characteristics

doi: 10.3724/SP.J.1141.2012.01060

Abstract: The tree shrews, as an ideal animal model receiving extensive attentions to human disease research, demands essential research tools, in particular cellular markers and monoclonal antibodies for immunological studies. In this paper, a 1 365 bp of the full-length CD4 cDNA encoding sequence was cloned from total RNA in peripheral blood of tree shrews, the sequence completes two unknown fragment gaps of tree shrews predicted CD4 cDNA in the GenBank database, and its molecular characteristics were analyzed compared with other mammals by using biology software such as Clustal W2.0 and so forth. The results showed that the extracellular and intracellular domains of tree shrews CD4 amino acid sequence are conserved. The tree shrews CD4 amino acid sequence showed a close genetic relationship with Homo sapiens and Macaca mulatta. Most regions of the tree shrews CD4 molecule surface showed positive charges as humans. However, compared with CD4 extracellular domain D1 of human, CD4 D1 surface of tree shrews showed more negative charges, and more two N-glycosylation sites, which may affect antibody binding. This study provides a theoretical basis for the preparation and functional studies of CD4 monoclonal antibody.

TIAN Wei-Wei, GAO Yue-Dong, GUO Yan, HUANG Jing-Fei, XIAO Chang, LI Zuo-Sheng, ZHANG Hua-Tang. Cloning of full-length coding sequence of tree shrew CD4 and prediction of its molecular characteristics. Zoological Research, 2012, 33(1): 60-66. doi: 10.3724/SP.J.1141.2012.01060
Citation: TIAN Wei-Wei, GAO Yue-Dong, GUO Yan, HUANG Jing-Fei, XIAO Chang, LI Zuo-Sheng, ZHANG Hua-Tang. Cloning of full-length coding sequence of tree shrew CD4 and prediction of its molecular characteristics. Zoological Research, 2012, 33(1): 60-66. doi: 10.3724/SP.J.1141.2012.01060
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