Volume 33 Issue 1
Jan.  2012
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WANG Wen-Guang, HUANG Xiao-Yan, XU Juan, SUN Xiao-Mei, DAI Jie-Jie, LI Qi-Han. Experimental studies on infant Tupaia belangeri chineses with EV71 infection. Zoological Research, 2012, 33(1): 7-13. doi: 10.3724/SP.J.1141.2012.01007
Citation: WANG Wen-Guang, HUANG Xiao-Yan, XU Juan, SUN Xiao-Mei, DAI Jie-Jie, LI Qi-Han. Experimental studies on infant Tupaia belangeri chineses with EV71 infection. Zoological Research, 2012, 33(1): 7-13. doi: 10.3724/SP.J.1141.2012.01007

Experimental studies on infant Tupaia belangeri chineses with EV71 infection

doi: 10.3724/SP.J.1141.2012.01007
  • Received Date: 2011-10-27
  • Rev Recd Date: 2012-01-10
  • Publish Date: 2012-02-22
  • Tupaia belangeri are small mammals with a squirrel-like appearance; they were formerly classified under the primates order despite the lack of derived features characteristic of primates. Given that T. belangeri are easy to raise, cheap to maintain, and have a small body size, a high reproductive rate, and close affinity to primates, these animals would be used as an alternative to primates in biomedical research. Three-month old T. belangeri chineses were infected with enterovirus 71 (EV71) via three different routes, namely, oral administration, nasal dripping, and tail intravenous injection, to study the infection in infant T. belangeri and find a feasible scheme to make them an ideal animal model of EV71 in place of primates. Daily activities were regularly observed, body temperatures were measured, and blood tests were conducted. Blood and fecal samples were regularly collected. The infection was examined via the neutralizing antibody test, reverse transcription polymerase chain reaction (RT-PCR), Real-Time PCR, and pathological analysis. The temperature, as well as the white blood cell count and the number of lymphocytes, increased four days after infection. Virus loads were determined in all three groups, and the peak appeared on, before, or after the tenth day, respectively. Thus, oral administration proved to be the best route. The highest serum antibody titer obtained was 1:16. Acute paralysis with urinary retention manifested after about two weeks, and pathological changes were observed in the brain, heart, lung, spleen, kidney, and other tissues. In conclusion, T. belangeri chineses can infected with EV71 via oral administration, nasal dripping, and tail intravenous injection. Therefore, T. belangeri are potential EV71 animal models for further studies on the mechanism of pathogenesis or vaccine evaluation.
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  • [1] Arita M, Nagata N, Iwata N, Ami Y, Suzaki Y, Mizuta K, Iwasaki T, Sata T, Wakita T, Shimizu H. 2007. An attenuated strain of enterovirus 71 belonging to genotype ashowed a broad spectrum of antigenicity with attenuated neurovirulence in cynomolgus monkeys[J]. J Virol, 81(17): 9386-9395.
    [2] Chen GQ, Shao RB, Wang HY, Chen YH. 2010. Research progress on pathogen and pathogenic molecular mechanisms of enterovirus 71[J]. Prog Mod Biomed, 10(19): 3795-3797. [陈国清, 邵荣标, 王海燕, 陈 玉宏. 2010. 肠道病毒EV71 病原学以及致病分子机制的研究进展. 现代生物医学进展, 10(19): 3795-3797.]
    [3] Chen YC, Yu CK, Wang YF, Liu CC, Su IJ, Lei HY. 2004. A murine oral enterovirus 71 infection model with central nervous system involvement[J]. J Gen Virol, 85(1): 69-77.
    [4] Dong C, Wang J, Liu L, Zhao H, Shi H, Zhang Y, Jiang L, Li Q. 2010. Optimized development of a candidate strain of inactivated EV71 vaccine and analysis of its immunogenicity in rhesus monkeys[J]. Hum Vaccin, 6(12): 1028-37.
    [5] Duo JY, Wang W, Tong W, Cong Z, Jiang H, Xu WB, Wei Q, Qin C. 2009. Experimental studies on ICR mice with EV71 infection[J]. Chn J CompMed, 19(5): 41-46. [朵建英, 王卫, 佟巍, 丛喆, 蒋虹, 许文波, 魏强, 秦川. 2009. 肠道病毒71 型(EV71)对ICR 小鼠的感染. 中国 比较医学杂志, 19(5): 41-46.]
    [6] Lin YW, Chang KC, Kao CM, et al. Chang SP, Tung YY, Chen SH. 2009. Lymphocyte and antibody responses reduce enterovirus 71 lethality in mice by decreasing tissue viral loads[J]. J Virol, 83(13): 6477-6483.
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Experimental studies on infant Tupaia belangeri chineses with EV71 infection

doi: 10.3724/SP.J.1141.2012.01007

Abstract: Tupaia belangeri are small mammals with a squirrel-like appearance; they were formerly classified under the primates order despite the lack of derived features characteristic of primates. Given that T. belangeri are easy to raise, cheap to maintain, and have a small body size, a high reproductive rate, and close affinity to primates, these animals would be used as an alternative to primates in biomedical research. Three-month old T. belangeri chineses were infected with enterovirus 71 (EV71) via three different routes, namely, oral administration, nasal dripping, and tail intravenous injection, to study the infection in infant T. belangeri and find a feasible scheme to make them an ideal animal model of EV71 in place of primates. Daily activities were regularly observed, body temperatures were measured, and blood tests were conducted. Blood and fecal samples were regularly collected. The infection was examined via the neutralizing antibody test, reverse transcription polymerase chain reaction (RT-PCR), Real-Time PCR, and pathological analysis. The temperature, as well as the white blood cell count and the number of lymphocytes, increased four days after infection. Virus loads were determined in all three groups, and the peak appeared on, before, or after the tenth day, respectively. Thus, oral administration proved to be the best route. The highest serum antibody titer obtained was 1:16. Acute paralysis with urinary retention manifested after about two weeks, and pathological changes were observed in the brain, heart, lung, spleen, kidney, and other tissues. In conclusion, T. belangeri chineses can infected with EV71 via oral administration, nasal dripping, and tail intravenous injection. Therefore, T. belangeri are potential EV71 animal models for further studies on the mechanism of pathogenesis or vaccine evaluation.

WANG Wen-Guang, HUANG Xiao-Yan, XU Juan, SUN Xiao-Mei, DAI Jie-Jie, LI Qi-Han. Experimental studies on infant Tupaia belangeri chineses with EV71 infection. Zoological Research, 2012, 33(1): 7-13. doi: 10.3724/SP.J.1141.2012.01007
Citation: WANG Wen-Guang, HUANG Xiao-Yan, XU Juan, SUN Xiao-Mei, DAI Jie-Jie, LI Qi-Han. Experimental studies on infant Tupaia belangeri chineses with EV71 infection. Zoological Research, 2012, 33(1): 7-13. doi: 10.3724/SP.J.1141.2012.01007
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