Volume 32 Issue 4
Jul.  2011
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YUE Ming-Jin, MO Sai-Jun, SONG Ping, GONG Yan-Zhang. Identification of Z-OTU protein during zebrafish oogenesis and early embryogenesis. Zoological Research, 2011, 32(4): 386-390. doi: 10.3724/SP.J.1141.2011.04386
Citation: YUE Ming-Jin, MO Sai-Jun, SONG Ping, GONG Yan-Zhang. Identification of Z-OTU protein during zebrafish oogenesis and early embryogenesis. Zoological Research, 2011, 32(4): 386-390. doi: 10.3724/SP.J.1141.2011.04386

Identification of Z-OTU protein during zebrafish oogenesis and early embryogenesis

doi: 10.3724/SP.J.1141.2011.04386
Funds:  This work was supported by grants from the ICGEB (International Center for Genetic Engineering and Biotechnology) (CRP/CHN02-01) (SONG Ping); the National Basic Research Program of China (2004CB117400) (SONG Ping); the National Natural Science Foundation of China (30150005; 30270675) (SONG Ping)
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  • Author Bio:

    YUE Ming-Jin,E-mail:ymjmsj0618@yahoo.com.cn

  • Corresponding author: SONG Ping, GONG Yan-Zhang
  • Received Date: 2010-11-08
  • Rev Recd Date: 2011-04-27
  • Publish Date: 2011-08-22
  • Zebrafish (Danio rerio) Z-OTU, containing OTU and TUDOR domains, was predicted to be a member of OTU-related protease, a family of the deubiquitylating enzymes (DUBs). A previous report from our laboratory clearly describes the expression patterns of z-otu mRNA. Here, we characterized the Z-OTU protein during zebrafish oogenesis and early embryogenesis. After prokaryotic expression, the recombinant protein of the OTU domain and GST was purified and injected into rabbits to obtain the polyclonal antibody-anti-Z-OTU, which was used for immunohistochemistry in zebrafish ovaries and embryos. Interestingly, obvious differences existed between the expression patterns of z-otu mRNA and its protein during oogenesis and early embryogenesis. In stage I oocytes, z-otu mRNA was detected in cytoplasm while its protein existed in the germinal vesicle. In addition, its protein was distributed during entire oogenesis, while mRNA was not detected in oocytes at stage IV or mature oocytes. The z-otu mRNA disappeared after midblastula transition (MBT) and its protein gradually decreased after this stage. We inferred that Z-OTU protein, like other OTU-related protease with DUB activity, was required for germinal vesicle breakdown of oocytes during meiosis, germinal vesicle migration, and embryo cleavage maintenance.
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  • [1]
    Balakirev MY, Tcherniuk SO, Jaquinod M, Chroboczek J. 2003. Otubains: a new family of cysteine proteases in the ubiquitin pathway [J]. EMBO Rep, 4: 517-522.
    [2]
     Ben-Yehoshua Josefsberg L, Kaufman O, Galiani D, Kovo M, Dekel N. 2001. Inactivation  of M-phase promoting factor at exit from first embryonic mitosis in the rat is independent of cyclin B1 degradation [J]. Biol Reprod, 64: 871-878.
    [3]
     Braat AK, van de, Water S, Goos H, Bogerd J, Zivkovic D. 2000. Vasa protein expression and localization in the zebrafish [J]. Mech Dev, 95: 271-274.
    [4]
     Dekel N. 1996. Protein phosphorylation/dephosphorylation in the meiotic cell cycle of mammalian oocytes [J]. Rev Reprod, 1: 82-88.
    [5]
     Evans PC, Smith TS, Lai MJ, Williams MG, Burke DF, Heyninck K, Kreike MM, Beyaert R, Blundell TL, Kilshaw PJ. 2003. A novel type of deubiquitinating enzyme
    [J]. J Biol Chem, 278: 23180-23186.
    [7]
     Evans PC, Ovaa H, Hamon M, Kilshaw PJ, Hamm S, Bauer S, Ploegh HL, Smith, TS. 2004. Zinc-finger protein A20, a regulator of inflammation and cell survival, has deubiquitinating activity [J]. Biochem J, 378: 727-734.
    [8]
     Huo LJ, Fan HY, Zhong ZS, Chen DY, Schatten H, Sun QY. 2004. Ubiquitin–proteasome pathway modulates mouse oocyte meiotic maturation and fertilization via regulation of MAPK cascade and cyclin B1 degradation [J]. Mech Dev, 121:1275-1287.
    [9]
     Jesuthasan S, Strahle U. 1997. Dynamic microtubules and specification of the zebrafish embryonic axis [J]. Curr Biol, 7: 31-42.
    [10]
     Knaut H, Pelegri F, Bohmann K, Schwarz H, Nüsslein-Volhard C. 2000. Zebrafish vasa RNA but not its protein is a component of the germ plasm and segregates asymmetrically before germline specification [J]. J Cell Biol, 149: 875-888.
    [11]
     Makarova KS, Aravind L, Koonin EV. 2000. A novel superfamily of predicted cysteine proteases from eukaryotes, viruses and Chlamydia 1 pneumoniae [J]. Trends Biochem Sci, 25: 50-52.
    [12]
     Mo S, Song P, Lü D, Chen Y, Zhou W, Gong W, Zhu Z. 2005. Zebrafish z-otu, a novel Otu and Tudor domain-containing gene, is expressed in early stages of oogenesis and embryogenesis [J]. Biochim Biophys Acta, 1732: 1-7.
    [13]
     Murray AW, Kirschner MW. 1989. Cyclin synthesis drives the early embryonic cell cycle [J]. Nature, 339: 275-280.
    [14]
     Opipari AW, Hu Jr HM, Yabkowitz R, Dixit VM. 1992. The A20 zinc finger protein protects cells from tumor necrosis factor cytotoxicity [J]. J Biol Chem, 267: 12424-12427.
    [15]
     Oppenheimer J. 1936. The development of isolated blastoderms of Fundulus heteroclitus [J]. J Exp Zool, 72: 247-269.
    [16]
     Pelegri F. 2003. Maternal factors in zebrafish development [J]. Dev Dyn, 228: 535-554.
    [17]
     Sun QY, Fuchimotoa D, Nagaia T. 2004. Regulatory roles of ubiquitin-proteasome pathway in pig oocyte meiotic maturation and fertilization [J]. Theriogenology, 62: 245-255.
    [18]
     Sieburth D, Ch'ng Q, Dybbs M, Tavazoie M, Kennedy S, Wang D, Dupuy D, Rual JF, Hill DE, Vidal M, Ruvkun G, Kaplan J. 2005. Systematic analysis of genes required for synapse structure and function [J]. Nature, 436: 510-517.
    [19]
     Tokumoto T, Yamashita M, Tokumoto M, Katsu Y, Horiguchi R, Kajiura H, Nagahama Y. 1997. Initiation of cyclin B degradation by the 26S proteasome upon egg activation
    [J]. J Cell Biol, 138: 1313-1322.
    [21]
     Uchiyama K Jokitalo E, Kano F, Murata M, Zhang XD, Canas B, Newman R,  Rabouille C, Pappin D, Freemont P, Kondo H. 2002. VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo [J]. J Cell Biol, 159: 855-866.
    [22]
     Wing SS. 2003. Deubiquitinating enzymes-the importance of driving in reverse along the ubiquitin–proteasome pathway [J]. Int J Biochem Cell Biol, 35: 590-605.
    [23]
     Xu HY, Gui JF, Hong YH. 2005. Differential Expression of vasa RNA and Protein During Spermatogenesis and Oogenesis in the Gibel Carp Carassius auratus gibelio., a Bisexually and Gynogenetically Reproducing Vertebrate [J]. Dev Dyn, 233: 872-882.
    [24]
     Yang Q, Hu J, Ye D, Zhao C, Song S, Gong W, Tan Z, Song P. 2010. Identification and expression analysis of two zebrafish E2F5 genes during oogenesis and development
    [J]. Mol Biol Rep, 37: 1773-1780.
    [26]
     
    [27]
     
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