Volume 32 Issue 4
Jul.  2011
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QI Zhi-Tao, ZHANG Qi-Huan, WANG Zi-Sheng, WANG Ai-Min, HUANG Bei, CHANG Ming-Xian. Cloning and expression analysis of a long type peptidoglycan recognition protein (PGRP-L) from Xenopus tropicalis. Zoological Research, 2011, 32(4): 371-378. doi: 10.3724/SP.J.1141.2011.04371
Citation: QI Zhi-Tao, ZHANG Qi-Huan, WANG Zi-Sheng, WANG Ai-Min, HUANG Bei, CHANG Ming-Xian. Cloning and expression analysis of a long type peptidoglycan recognition protein (PGRP-L) from Xenopus tropicalis. Zoological Research, 2011, 32(4): 371-378. doi: 10.3724/SP.J.1141.2011.04371

Cloning and expression analysis of a long type peptidoglycan recognition protein (PGRP-L) from Xenopus tropicalis

doi: 10.3724/SP.J.1141.2011.04371
Funds:  This study was financially supported by the Project from the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (10KJB240001), the Foundation for Talent Recruitment of Yancheng Institute of Technology (XKR2011007), and the National Natural Science Foundation of China (30830083)
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  • Author Bio:

    QI Zhi-Tao1

  • Corresponding author: QI Zhi-Tao
  • Received Date: 2011-03-15
  • Rev Recd Date: 2011-06-27
  • Publish Date: 2011-08-22
  • Peptidoglycan recognition proteins (PGRPs) are a family of pattern recognition receptors (PRRs) of the immune system, which bind and hydrolyze bacterial peptidoglycan. Here, a long type PGRP (PGRP-L) was first cloned in the lower vertebrate species Xenopus tropicalis (Xt). The XtPGRP-L possessed a conserved genomic structure with five exons and four introns. The alignment and phylogenetic analysis indicated that XtPGRP-L might be a type of amidase-like PGRP. The 3-D model showed that XtPGRP-L possessed a conserved structure compared with the Drosophila PGRP-Lb. During embryonic development, XtPGRP-L was not expressed until the 72 h tadpole stage. In adult tissues, it was strongly expressed in the liver, lung, intestine, and stomach. Furthermore, after LPS stimulation, the expression of XtPGRP-L was up-regulated significantly in the liver, intestine and spleen, indicating that XtPGRP-L may play an important role in the innate immunity of Xenopus tropicalis.
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