Volume 32 Issue 4
Jul.  2011
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YANG Hui, HUANG Yuan. Analysis of the complete mitochondrial genome sequence of Pielomastax zhengi. Zoological Research, 2011, 32(4): 353-362. doi: 10.3724/SP.J.1141.2011.04353
Citation: YANG Hui, HUANG Yuan. Analysis of the complete mitochondrial genome sequence of Pielomastax zhengi. Zoological Research, 2011, 32(4): 353-362. doi: 10.3724/SP.J.1141.2011.04353

Analysis of the complete mitochondrial genome sequence of Pielomastax zhengi

doi: 10.3724/SP.J.1141.2011.04353
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  • Author Bio:

    YANG Hui

  • Corresponding author: HUANG Yuan
  • Received Date: 2011-01-06
  • Rev Recd Date: 2011-03-28
  • Publish Date: 2011-08-22
  • ThecompletemitochondrialgenomesequenceofPielomastaxzhengiwasdeterminedbyusinglongPCRandconservedprimerwalkingapproaches.TheresultsshowedthattheentiremitochondrialgenomeofPielomastaxzhengiis15602bplongwithA+Tcontentof71.8%.All37genesareconservedinthepositionsobservedinthoseofLocustamigratoria.Allthegenesarecloselyassembledbyleaving10intergenicspacersinbetween.Thoseintergenicspacersare47bpintotal(excludingtheA+Trichregion),withindividualsizerangesfrom1bpto20bp.Inaddition,thereareatotalof52bpoverlappingsequencesamong14genes,rangingfrom1−8bp.Allprotein-codinggenesstartwithatypicalinitiationcodonininsects,ATN.Twelveprotein-codinggenesusetheusualTAAandTAGterminationcodons,whereas,theND5geneshaveanincompleteterminationcodon(T).ExceptthetRNASer(AGN),whoseDHUarmisabsent;alltheother21tRNAgeneshavetypicalclover-leafsecondarystructures.ButinPielomastaxzhengi,thesecondarystructuresoffivetRNA(tRNACys,tRNALys,tRNAPhe,tRNAPro,tRNAArg)genescannotbepredictedbytheconventionalmethodsastheyhaveonly3−4,ratherthan5basepairsintheTψCarm,while,thetRNALysandtRNAArghaveonly4basepairsintheanticodearm.ThepredictedsecondarystructuresoflrRNAandsrRNAhave6domainswith44helicesand3domainswith30helices,respectively.TheresultsoftherRNAsecondarystructurecomparisonshowedthatPielomastaxzhengiismorecloselyrelatedwithLocustamigratoriatibetensisthanRuspoliadubia.Likemost&
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