Volume 32 Issue 3
May  2011
Turn off MathJax
Article Contents
CHEN Wen-Bo, WANG Xin, ZHOU Ya-Lu, DONG Hai-Yan, LIN Hao-Ran, LI Wen-Sheng. Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus). Zoological Research, 2011, 32(3): 285-292. doi: 10.3724/SP.J.1141.2011.03285
Citation: CHEN Wen-Bo, WANG Xin, ZHOU Ya-Lu, DONG Hai-Yan, LIN Hao-Ran, LI Wen-Sheng. Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus). Zoological Research, 2011, 32(3): 285-292. doi: 10.3724/SP.J.1141.2011.03285

Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus)

doi: 10.3724/SP.J.1141.2011.03285
More Information
  • Author Bio:

    CHEN Wen-Bo,WANG Xin

    CHEN Wen-Bo,WANG Xin

  • Corresponding author: LI Wen-Sheng
  • Received Date: 2010-09-13
  • Rev Recd Date: 2011-02-21
  • Publish Date: 2011-06-22
  • We cloned the full length of tilapia prepro-orexin cDNA using RT-PCR and rapid-amplification of cDNA ends (RACE). The full-length of prepro-orexin cDNA was 648 bp containing an open reading frame of 423 bp. The 140 amino acid prepro-orexin protein included a 37 AA signal peptide, a 43 AA Orexin-A and, and 28 AA Orexin-B and the end of the 32 AA peptide of unknown function. The expression of prepro-orexin on tissue distribution, peri-prandial changes, starvation and re-feeding were quantified by real-time PCR. We found that prepro-orexin mRNA was present in all tissues tested and that the highest level was observed in hypothalamus. Expression levels were significantly higher at mealtime (0 h) than before (−2 h, −1 h) and after (+1 h, +2 h) mealtime. Fasting for 2, 4, 6 and 8 d caused significant increases in prepro-orexin mRNA expression in the hypothalamus, and after re-feeding, expression levels of prepro-o rexin mRNA returned to the same level compared to that in the fed group.
  • loading
  • [1]
    Adamantidis A, de Lecea L. 2008. Physiological arousal: A role for hypothalamic systems[J]. Cell Mol Life Sci, 65(10): 1475-1488.Alvarez CE, Sutcliffe JG. 2002. Hypocretin is an early member of the incretin gene family[J]. Neurosci Lett, 324(3): 169-172.Buckley C, MacDonalda EE, Tuziaka SM, Volkoff H. 2010. Molecular cloning and characterization of two putative appetite regulators in winter flounder (Pleuronectes americanus): Preprothyrotropin-releasing hormone (TRH) and prepro-orexin (OX)[J]. Peptides, 2010, 31(9): 1737-1747.Caillol M, Aioun J, Baly C, Persuy MA, Salesse R. 2003. Localization of orexins and their receptors in the rat olfactory system: Possible modulation of olfactory perception by a neuropeptide synthetized centrally or locally[J]. Brain Res, 960(1-2): 48-61.Chen WB, Li WS, Lin HR. 2009. Common carp (Cyprinus carpio) insulin-like growth factor binding protein-2 (IGFBP-2): molecular cloning, expression profiles, and hormonal regulation in hepatocytes[J]. Gen Comp Endocrinol,161(3): 390-399.Dyer CJ, Touchette KJ, Carroll JA, Allee GL, Matteri RL. 1999. Cloning of porcine prepro-orexin cDNA and effects of an intramuscular injection of synthetic porcine Orexin-B on feed intake in young pigs[J]. Domest Anim Endocrinol, 16(3):145-148.de Lecea L, Kilduff TS, Peyron C, Gao X, Foye PE, Danielson PE, Fukuhara C, Battenberg EL, Gautvik VT, Bartlett FS, 2nd, Frankel WN, van den Pol AN, Bloom FE, Gautvik KM, Sutcliffe JG. 1998. The hypocretins:hypothalamus-specific peptides with neuroexcitatory activity[J]. Proc Natl Acad Sci USA, 95(1): 322-327.Faraco JH, Appelbaum L, Marin W, Gaus SE, Mourrain P, Mignot E. 2006. Regulation of hypocretin (Orexin) expression in embryonic zebrafish[J]. J Biol Chem,281(40): 29753-29761.Johren O, Neidert SJ, Kummer M, Dendorfer A, Dominiak P. 2001. Prepro-orexin and orexin receptor mRNAs are differentially expressed in peripheral tissues of male and female rats[J]. Endocrinology, 142(8):3324-3331.Kane JK, Parker SL, Matta SG, Fu Y, Sharp BM, Li MD. 2000. Nicotine up-regulates expression of orexin and its receptors in rat brain[J]. Endocrinology, 141(10): 3623-3629.Kaslin J, Nystedt JM, Ostergard M, Peitsaro N, Panula P. 2004. The orexin/ hypocretin system in zebrafish is connected to the aminergic and cholinergic systems
    [J]. J Neurosci, 24(11): 2678-2689.Kirchgessner AL. 2002. Orexins in the brain-gut axis[J]. Endocr Rev, 23(1): 1-15.Kirchgessner AL, Liu M. 1999. Orexin synthesis and response in the gut[J]. Neuron, 24(4): 941-951.Kehoe AS, Volkoff H. 2007. Cloning and characterization of neuropeptide Y (NPY) and cocaine and amphetamine regulated transcript (CART) in Atlantic cod (Gadus morhua)
    [J]. Comp Biochem Physiol A: Mol Integr Physiol, 146(3): 451-461.Matsuki T, Sakurai T. 2008. Orexins and orexin receptors: From molecules to integrative physiology [J]. Results Probl Cell Differ,46: 27-55.Miura T, Maruyama K, Shimakura S, Kaiya H, Uchiyama M, Kangawa K, Shioda S, Matsuda K. 2007. Regulation of food intake in the goldfish by interaction between ghrelin and orexin[J]. Peptides, 28(6):1207-1213.Ohno K, Sakurai T. 2008. Orexin neuronal circuitry: role in the regulation of sleep and wakefulness[J]. Front Neuroendocrinol, 29(1): 70-87.Sakurai T, Amemiya A, Ishii M, Matsuzaki I, Chemelli RM, Tanaka H, Williams SC, Richardson JA, Kozlowski GP, Wilson S, Arch JR, Buckingham RE, Haynes AC, Carr SA, Annan RS, McNulty DE, Liu WS, Terrett JA, Elshourbagy NA, Bergsma DJ, Yanagisawa M. 1998. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior[J]. Cell, 92(4): 573-585.Tan XM, Chen DW, Yang YF. 2004. Orexin and its physiological functions[J]. Chn Feed, 14: 2-7 [谭雪梅, 陈代文, 羊云飞. 增食欲素及其生理功能. 中国饲料, 2004, 14: 2-7]Xu M, Volkoff H. 2007. Molecular characterization of prepro-orexin in Atlantic cod (Gadus morhua): cloning, localization, developmental profile and role in food intake regulation[J]. Mol Cell Endocrinol, 271(1-2): 28-37.Unniappan S, Canosa LF, Peter RE. 2004. Orexigenic actions of ghrelin in goldfish: Feeding-induced changes in brain and gut mRNA expression and serum levels, and responses to central and peripheral injections[J]. Neuroendocrinology, 79(2):100-108.Volkoff H, Canosa LF, Unniappan S, Cerda-Reverter JM, Bernier NJ, Kelly SP, Peter RE. 2005. Neuropeptides and the control of food intake in fish [J]. Gen Comp Endocrinol, 142(1-2): 3-19.Volkoff H, Peter RE. 2001. Characterization of two forms of cocaine- and amphetamine-regulated transcript (CART) peptide precursors in g488 oldfish: molecular cloning and distribution, modulation of expression by nutritional status, and interactions with leptin [J]. Endocrinology, 142(12): 5076-5088.Volkoff H, Eykelbosh AJ, Peter RE. 2003. Role of leptin in the control of feeding of goldfish Carassius auratus: Interactions with cholecystokinin, neuropeptide Y and Orexin A, and modulation by fasting[J]. Brain Res, 972(1-2):90-109.Volkoff H, Bjorklund JM, Peter RE. 1999. Stimulation of feeding behavior and food consumption in the goldfish, Carassius auratus, by Orexin-A and Orexin-B [J]. Brain Res, 846(2): 204-209.Zhang LJ.2006.Prepro-orexin of Orange-spotted Grouper, Epinephelus coioides: Molecular Cloning and Characteristics of mRNA Expression
    [D]. Guanzhou: Sun Yet-sen University.

  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2053) PDF downloads(1760) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint