Volume 32 Issue 3
May  2011
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CHEN Wen-Bo, WANG Xin, ZHOU Ya-Lu, DONG Hai-Yan, LIN Hao-Ran, LI Wen-Sheng. Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus). Zoological Research, 2011, 32(3): 285-292. doi: 10.3724/SP.J.1141.2011.03285
Citation: CHEN Wen-Bo, WANG Xin, ZHOU Ya-Lu, DONG Hai-Yan, LIN Hao-Ran, LI Wen-Sheng. Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus). Zoological Research, 2011, 32(3): 285-292. doi: 10.3724/SP.J.1141.2011.03285

Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus)

doi: 10.3724/SP.J.1141.2011.03285
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  • Author Bio:

    CHEN Wen-Bo,WANG Xin

    CHEN Wen-Bo,WANG Xin

  • Corresponding author: LI Wen-Sheng
  • Received Date: 2010-09-13
  • Rev Recd Date: 2011-02-21
  • Publish Date: 2011-06-22
  • We cloned the full length of tilapia prepro-orexin cDNA using RT-PCR and rapid-amplification of cDNA ends (RACE). The full-length of prepro-orexin cDNA was 648 bp containing an open reading frame of 423 bp. The 140 amino acid prepro-orexin protein included a 37 AA signal peptide, a 43 AA Orexin-A and, and 28 AA Orexin-B and the end of the 32 AA peptide of unknown function. The expression of prepro-orexin on tissue distribution, peri-prandial changes, starvation and re-feeding were quantified by real-time PCR. We found that prepro-orexin mRNA was present in all tissues tested and that the highest level was observed in hypothalamus. Expression levels were significantly higher at mealtime (0 h) than before (−2 h, −1 h) and after (+1 h, +2 h) mealtime. Fasting for 2, 4, 6 and 8 d caused significant increases in prepro-orexin mRNA expression in the hypothalamus, and after re-feeding, expression levels of prepro-o rexin mRNA returned to the same level compared to that in the fed group.
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