Volume 32 Issue 3
May  2011
Turn off MathJax
Article Contents
FENG Li-Fang, CHANG Yue, YUAN Dong-Xia, MIAO Wei. Expression analysis of 5 hsp70 genes in Tetrahymena thermophila. Zoological Research, 2011, 32(3): 267-276. doi: 10.3724/SP.J.1141.2011.03267
Citation: FENG Li-Fang, CHANG Yue, YUAN Dong-Xia, MIAO Wei. Expression analysis of 5 hsp70 genes in Tetrahymena thermophila. Zoological Research, 2011, 32(3): 267-276. doi: 10.3724/SP.J.1141.2011.03267

Expression analysis of 5 hsp70 genes in Tetrahymena thermophila

doi: 10.3724/SP.J.1141.2011.03267
More Information
  • Author Bio:

    FENG Li-Fang

  • Corresponding author: MIAO Wei
  • Received Date: 2010-10-13
  • Rev Recd Date: 2011-02-22
  • Publish Date: 2011-06-22
  • Thirteen hsp70 genes with complete conserved domains were identified in Tetrahymena thermophila, and expression of five similar and non-intron hsp70 genes were analyzed. Under heat shock conditions of 37, 39 and 41ºC, hsp70-2 mRNA had the highest relative expression level, suggesting it is closely related to heat shock. The basal level of constitutive T. thermophilahsp70-4 gene was high during 20 physiological/developmental stages of growth, starvation and conjugation, and it changed little upon exposure to heat shock: evidence that hsp70-4 is an hsc70 gene. The hsp70-4 cDNA is 2 208 bp long, and contains an open reading frame of 1 959 bp encoding 635 amino acids. Microarray data indicated that T. thermophilahsp70-3 gene probably participated in early starvation (0–12 h) stress and late conjugation (6–10 h) events, such as new macronuclear and micronuclear anlagen formation and old macronuclear elimination. However, hsp70-5 gene possibly participates in late starvation (12–15 h) stress and early conjugation (0–6 h) events such as micronuclear meiosis, micronuclear exchange and pronuclear fusion. Blast2GO indicated that they participated in dissimilar biological processes, suggesting hsp70-3 and hsp70-5 perform different functions.
  • loading
  • [1]
    Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    [J].Nucleic Acids Res, 25(17): 3389-3402.Barchetta S, La Terza A, Ballarini P, Pucciarelli S, Miceli C. 2008. Combination of two regulatory elements in the Tetrahymena thermophila HSP70-1 gene controls heat shock activation [J]. Eukaryot cell, 7(2): 379-386.Bettencourt BR, Feder ME. 2001. Hsp70 duplication in the Drosophila melanogaster species group: how and when did two become five? [J]. Mol Biol Evol, 18(7): 1272-1282.Boldrin F, Santovito G, Negrisolo E, Piccinni E. 2003. Cloning and sequencing of four new metallothionein genes from Tetrahymena thermophila and T. pigmentosa: evolutionary relationships in Tetrahymena MT family [J]. Protist, 154(3-4): 431-42.Bonato MC, Silva AM, Gomes SL, Maia JC, Juliani MH. 1987. Differential expression of heat-shock proteins and spontaneous synthesis of HSP70 during the life cycle of Blastocladiella emersonii [J]. Eur J Biochem, 163(1): 211-220.Capella-Gutierrez S, Silla-Martinez JM, Gabaldon T. 2009. TrimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses [J]. Bioinformatics, 25(15): 1972-1973.Chou CC, Forouhar F, Yeh YH, Shr HL, Wang C, Hsiao CD. 2003. Crystal structure of the C-terminal 10-kDa subdomain of Hsc70 [J]. J Biol Chem, 278(32): 30311-30316.Chughtai ZS, Rassadi R, Matusiewicz N, Stochaj U. 2001. Starvation promotes nuclear accumulation of the hsp70 Ssa4p in yeast cells
    [J]. J Biol Chem, 276(23): 20261-20266.Cole ES, Cassidy-Hanley D, Hemish J, Tuan J, Bruns PJ. 1997. A mutational analysis of conjugation in Tetrahymena thermophila. 1. Phenotypes affecting early development: meiosis to nuclear selection [J]. Dev Biol, 189(2): 215-232.Cole ES, Soelter TA. 1997. A mutational analysis of conjugation in Tetrahymena thermophila. 2. Phenotypes affecting middle and late development: third prezygotic nuclear division, pronuclear exchange, pronuclear fusion, and postzygotic development. Dev Biol, 189(2): 233-245.Colinet H, Lee SF, Hoffmann A. 2010. Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster
    [J]. FEBS J, 277(1): 174-185.Craig EA, Gambill BD, Nelson RJ. 1993. Heat shock proteins: molecular chaperones of protein biogenesis [J]. Microbiol Rev, 57(2): 402-414.Daugaard M, Rohde M, Jäättelä M. 2007. The heat shock protein 70 family: Highly homologous proteins with overlapping and distinct functions [J]. FEBS Lett, 581(19): 3702-3710.Demand J, Lüders J, Höhfeld J. 1998. The carboxy-terminal domain of Hsc70 provides binding sites for a distinct set of chaperone cofactors [J]. Mol Cell Biol, 18(4): 2023-2028.Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput [J]. Nucleic Acids Res, 32(5): 1792-1797.Eisen JA, Coyne RS, Wu M, Wu D, Thiagarajan M, Wortman JR, Badger JH, Ren Q, Amedeo P, Jones KM, Tallon LJ, Delcher AL, Salzberg SL, Silva JC, Haas BJ, Majoros WH, Farzad M, Carlton JM, Smith RK Jr, Garg J, Pearlman RE, Karrer KM, Sun L, Manning G, Elde NC, Turkewitz AP, Asai DJ, Wilkes DE, Wang Y, Cai H, Collins K, Stewart BA, Lee SR, Wilamowska K, Weinberg Z, Ruzzo WL, Wloga D, Gaertig J, Frankel J, Tsao CC, Gorovsky MA, Keeling PJ, Waller RF, Patron NJ, Cherry JM, Stover NA, Krieger CJ, del Toro C, Ryder HF, Williamson SC, Barbeau RA, Hamilton EP, Orias E. 2006. Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote [J]. PLoS Biol, 4(9): e286.Feng LF, Miao W, Wu YX. 2007. Differentially expressed genes of Tetrahymena thermophila in response to tributyltin (TBT) identified by suppression subtractive hybridization and real time quantitative PCR [J]. Aquat Toxicol, 81(1): 99-105.Feng LF, Miao W. 2008. Comparative analysis of the Hsp70 mRNA expression and heat tolerance in two regional Carchesium polypinum at different latitudes
    [J]. Acta Zool Sin, 54(3): 525-530. [冯立芳, 缪炜. 2008. 不同纬度螅状独缩虫耐热能力及Hsp70 mRNA表达水平的比较. 动物学报, 54(3): 525-530.]Finn RD, Mistry J, Tate J, Coggill P, Heger A, Pollington JE, Gavin OL, Gunasekaran P, Ceric G, Forslund K, Holm L, Sonnhammer EL, Eddy SR, Bateman A. 2010. The Pfam protein families database [J]. Nucleic Acids Res, 38(Database issue): D211-D22.Frankel J, Nelsen EM. 2001. The effects of supraoptimal temperatures on population growth and cortical patterning in Tetrahymena pyriformis and Tetrahymena thermophila: a comparison [J]. J Eukaryot Microbiol, 48(2): 135-146.Fu C, Xiong J, Miao W. 2009. Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila
    [J]. BMC Genomics, 10: 208.Galego L, Rodrigues-Pousada C. 1985. Regulation of gene expression in Tetrahymena pyriformis under heat-shock and during recovery [J]. Eur J Biochem, 149(3): 571-578.Georg Rde C, Gomes SL. 2007. Comparative expression analysis of members of the Hsp70 family in the chytridiomycete Blastocladiella emersonii [J]. Gene, 386(1-2): 24-34.Guo LN, Yu T, Feng LF, Miao W. 2010. Cloning and expression analysis of Tetrahymena 2-oxoglutarate/malate carrier gene (omc) exposed to dichlorodiphenyltrichloroethane (DDT) [J]. Chn J Appl Environ Biol, 16(2): 216-221. [郭利娜, 俞婷, 冯立芳, 缪炜. 2010. 四膜虫omc基因的克隆及在滴滴涕(DDT)暴露下的表达分析. 应用与环境生物学报, 16(2): 216-221.]Hallberg RL, Kraus KW, Findly RC. 1984. Starved Tetrahymena thermophila cells that are unable to mount an effective heat shock response selectively degrade their rRNA
    [J]. Mol Cell Biol, 4(10): 2170-2179.Hallberg RL, Kraus KW, Hallberg EM. 1985. Induction of acquired thermotolerance in Tetrahymena thermophila: effects of protein synthesis inhibitors [J]. Mol Cell Biol, 5(8): 2061-2069.Karouna-Renier NK, Yang WJ, Rao KR. 2003. Cloning and characterization of a 70 kDa heat shock cognate gene (HSC70) from two species of Chironomus [J]. Insect Mol Biol, 12(1): 19-26.Katoh K, Toh H. 2008. Recent developments in the MAFFT multiple sequence alignment program [J]. Brief Bioinform, 9(4): 286-298.Kiang JG, Tsokos GC. 1998. Heat shock protein 70 kDa: molecular biology, biochemistry, and physiology [J]. Pharmacol Ther, 80(2): 183-201.Lindquist S, Craig EA. 1998. The heat-shock proteins [J]. Annu Rev Genet, 22: 631-677.Marchler-Bauer A, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, Geer LY, Geer RC, Gonzales NR, Gwadz M, He S, Hurwitz DI, Jackson JD, Ke Z, Lanczycki CJ, Liebert CA, Liu C, Lu F, Lu S, Marchler GH, Mullokandov M, Song JS, Tasneem A, Thanki N, Yamashita RA, Zhang D, Zhang N, Bryant SH. 2009. CDD: specific functional annotation with the Conserved Domain Database [J]. Nucleic Acids Res, 37(Database issue): D205-D210.Miao W, Xiong J, Bowen J, Wang W, Liu Y, Braguinets O, Grigull J, Pearlman RE, Orias E, Gorovsky MA. 2009. Microarray analyses of gene expression during the Tetrahymena thermophila life cycle [J]. PLoS One, 4(2): e4429.Nanney DL, Simon EM. 2000. Laboratory and Evolutionary History of Tetrahymena thermophila [M]// Forney JD. Tetrahymena thermophila. San Deigo: Academic Press, 3-25.Pfaffl MW, Horgan GW, Dempfle L. 2002. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR [J]. Nucleic Acids Res, 30(9): e36.Price MN, Dehal PS, Arkin AP. 2010. FastTree 2 - approximately maximum- likelihood trees for large alignments [J]. PLoS One, 5(3): e9490.Shen YF. 1999. Ciliophora [M]//Protozoology. Beijing: Science Press, 454-456. [沈韫芬. 1999. 纤毛门[M]//原生动物学. 北京:科学出版社, 454-456.]Snutch TP, Heschl MF, Baillie DL. 1988. The Caenorhabditis elegans hsp70 gene family: a molecular genetic characterization [J]. Gene, 64(2): 241-255.Sung DY, Vierling E, Guy CL. 2001. Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family [J]. Plant Physiol, 126(2): 789-800.Wang TF, Chang JH, Wang C. 1993. Identification of the peptide binding domain of hsc70. 18-Kilodalton fragment located immediately after ATPase domain is sufficient for high affinity binding [J]. J Biol Chem, 268(35): 26049-26051.Williams NE, Nelsen EM. 1997. HSP70 and HSP90 homologs are associated with tubulin in hetero-oligomeric complexes, cilia and the cortex of Tetrahymena
    [J]. J Cell Sci, 110 (Pt 14): 1665-1672.Yengkokpam S, Pal AK, Sahu NP, Jain KK, Dalvi R, Misra S, Debnath D. 2008. Metabolic modulation in Labeo rohita fingerlings during starvation: Hsp70 expression and oxygen consumption [J]. Aquaculture, 285(1-4): 234-237.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2160) PDF downloads(1632) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint