Volume 31 Issue 5
Sep.  2010
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LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 2010, 31(5): 483-489. doi: 10.3724/SP.J.1141.2010.05483
Citation: LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 2010, 31(5): 483-489. doi: 10.3724/SP.J.1141.2010.05483

Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics

doi: 10.3724/SP.J.1141.2010.05483
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  • Author Bio:

    LI Yi-Jiang,E-mail: liyijiang88.student@sina.com

  • Received Date: 2010-01-22
  • Rev Recd Date: 2010-09-15
  • Publish Date: 2010-10-22
  • The use of tree shrews (Tupaia belangeri) in human disease studies demands essential research tools, in particular cellular markers and their monoclonal antibodies for immunological studies. Here we cloned the full-length cDNAs encoding CD3ε from total RNA of the spleen, liver and peripheral blood of tree shrews and analyzed their structural characteristics in comparison with other mammals by Discovery Studio software. The results showed that the open reading frame sequence of tree shrew CD3ε was 582 bp, encoding 194 amino acids. The overall structure of tree shrew CD3ε protein was similar to its counterparts of other mammals, intracellular and transmembrane domain highly conserved. However, detailed analysis revealed two potential glycosylation sites and different surface charges in the extracellular domain. Availability of the entire open-reading-frame and related sequence information would therefore facilitate the preparation of monoclonal antibodies against tree shrew CD3 and further studies for its function.
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  • [1] Borroto A, Lama J, Niedergang F, Dautry-Varsat A, Alarcón B, Alcover A. 1999. The CD3 epsilon subunit of the TCR contains endocytosis signals [J].J Immunol,163(1): 25-31.Cao J, Yang EB, Su JJ, Li Y, Chow P. 2003. The tree shrews: Adjuncts and alternatives to primates as models for biomedical research [J]. J Med Primatol, 32(3): 123-130.Darai G, Zöller L, Matz B, Schwaier A, Flügel RM, Munk K. 1980. Experimental infection and the state of viral latency of adult tupaia with herpes simplex virus type 1 and 2 and infection of juvenile tupaia with temperature-sensitive mutants of HSV Type 2 [J]. Arch Virol,65(3-4): 311-318.Gong FL. 2009. Medical Immunology [M]. 3rd ed . Beijing: Science Press, 64-65. [龚非力. 2009. 医学免疫学[M]. 3版. 北京: 科学出版社, , 64-65.]Jacobs H, Vandeputte D, Tolkamp L, de Vries E, Borst J, Berns A. 1994. CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo [J].Eur J Immunol,24(4): 934-939.Kampen MV, Kramer M, Hiemke C, Flügge G, Fuchs E. 2002. The chronic psychosocial stress paradigm in male tree shrews: Evaluation of a novel animal model for depressive disorders [J]. Stress, 5(1): 37-46.Koch CA, Anderson D, Moran MF, Ellis C, Pawson T. 1991. SH2 and SH3 domains: Elements that control interactions of cytoplasmic signaling proteins [J]. Science, 252(5006): 668-674.Myung PS, Boerthe NJ, Koretzky GA. 2000. Adapter proteins in lymphocyte antigen-receptor signaling [J]. Curr Opin Immunol, 2(3): 256-266.Nie W, Fu B, O'Brien PC, Wang J, Su W, Tanomtong A, Volobouev V, Ferguson-Smith MA, Yang F. 2008. Flying lemurs—the ‘flying tree shrews'? Molecular cytogenetic evidence for a Scandentia-Dermoptera sister clade [J].BMC Biol,6: 18.Novacek MJ. 1992. Mammalian phylogeny: Shaking the tree [J]. Nature,356(6365): 121-125.Samelson LE. 2002. Signal transduction mediated by the T cell antigen receptor: the role of adapter proteins [J].Annu Rev Immunol,20: 371-394.San José E, Sahuquillo AG, Bragado R, Alarcón B. 1998. Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 epsilon/gamma dimers associate indistinctly with both TCR alpha and TCR beta chains. Evidence for a double TCR heterodimer model [J].Eur J Immunol,28(1): 12-21.Waddell PJ, Kishino H, Ota R. 2001. A phylogenetic foundation for comparative mammalian genomics [J]. Genome Inform, 12: 141-154.Walter E, Keist R, Niederöst B, Pult I, Blum HE. 1996.Hepatitis B virus infection of tupaia hepatocytes in vitro and in vivo [J]. Hepatology,24(1): 1-5.Wang B, Levelt C, Salio M, Zheng D, Sancho J, Liu CP, She J, Huang M, Higgins K, Sunshine MJ. 1995. Over-expression of CD3 epsilon transgenes blocks T lymphocyte development [J]. Int Immunol, 7(3): 435-448.Zhao X, Tang ZY, Klumpp B, Wolff-Vorbeck G, Barth H, Levy S, von Weizsäcker F, Blum HE, Baumert TF. 2002. Primary hepatocytes of Tupaia belangeri as a potential model for hepatitis C virus infection [J]. J Clin Invest,109(2): 221-232.
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Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics

doi: 10.3724/SP.J.1141.2010.05483
  • Author Bio:

Abstract: The use of tree shrews (Tupaia belangeri) in human disease studies demands essential research tools, in particular cellular markers and their monoclonal antibodies for immunological studies. Here we cloned the full-length cDNAs encoding CD3ε from total RNA of the spleen, liver and peripheral blood of tree shrews and analyzed their structural characteristics in comparison with other mammals by Discovery Studio software. The results showed that the open reading frame sequence of tree shrew CD3ε was 582 bp, encoding 194 amino acids. The overall structure of tree shrew CD3ε protein was similar to its counterparts of other mammals, intracellular and transmembrane domain highly conserved. However, detailed analysis revealed two potential glycosylation sites and different surface charges in the extracellular domain. Availability of the entire open-reading-frame and related sequence information would therefore facilitate the preparation of monoclonal antibodies against tree shrew CD3 and further studies for its function.

LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 2010, 31(5): 483-489. doi: 10.3724/SP.J.1141.2010.05483
Citation: LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 2010, 31(5): 483-489. doi: 10.3724/SP.J.1141.2010.05483
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