Volume 31 Issue 5
Sep.  2010
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ZHANG Ying, YU Jian-Feng, YANG Li, WANG Xing-Guo, GU Zhi-Liang. Cloning and characterization of Caveolin-1 gene in pigeon,Columba livia domestica. Zoological Research, 2010, 31(5): 476-482. doi: 10.3724/SP.J.1141.2010.05476
Citation: ZHANG Ying, YU Jian-Feng, YANG Li, WANG Xing-Guo, GU Zhi-Liang. Cloning and characterization of Caveolin-1 gene in pigeon,Columba livia domestica. Zoological Research, 2010, 31(5): 476-482. doi: 10.3724/SP.J.1141.2010.05476

Cloning and characterization of Caveolin-1 gene in pigeon,Columba livia domestica

doi: 10.3724/SP.J.1141.2010.05476
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  • Author Bio:

    ZHANG Ying;E-mail: yingzhang0928@yahoo.cn

  • Corresponding author: GU Zhi-Liang
  • Received Date: 2010-02-08
  • Rev Recd Date: 2010-09-09
  • Publish Date: 2010-10-22
  • Caveolins, a class ofprincipal proteins forming the structure of caveolae in plasmalemma, were encoded by caveolins gene family. Caveolin-1 gene is a member of caveolins gene family. In the present study, a full-length of 2605 bp caveolin-1 cDNA sequence in Columba livia domestica, which included a 537 bp complete ORF encoding a 178 amino acids long putative peptide, were obtained by using RT-PCR and RACE technique. The Columba livia domestica caveolin-1CDS shared 80.1% − 93.4% homology with Bos taurus, Canis lupus familiaris, Gallus gallus and Rattus norvegicus. Meanwhile, the putative amino acid sequence of Columba livia domestica caveolin-1 shared 85.4% − 97.2% homology with the above species. The semi-quantity RT-PCR revealed that Caveolin-1expressions were detectable in all the Columba livia domestica tissues and the expressional level of caveolin-1 gene was high in adipose, medium in various muscles, low in liver. These results demonstrated that Caveolin-1 gene was potentially involved in some metabolic pathways in adipose and muscle.
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  • [1] Cohen AW, Park DS, Woodman SE, Williams TM, Chandra M, Shirani J, Pereira de Souza A, Kitsis RN, Russell RG, Weiss LM, Tang B, Jelicks LA, Factor SM, Shtutin V, Tanowitz HB, Lisanti MP. 2003. Caveolin-1 null mice develop cardiac hypertrophy with hyperactivation of p42/44 MAP kinase in cardiac fibroblasts [J]. Am J Physiol Cell Physiol, 284(2): 457-474.Cohen AW, Razani B, Wang XB, Combs TP, Williams TM, Scherer PE, Lisanti MP. 2003. Caveolin-1-deficient mice show insulin resistance and defective insulin receptor protein expression in adipose tissue [J]. Am J Physiol Cell Physiol, 285(1): 222-235.Engelman JA, Zhang XL, Lisanti MP. 1998. Genes encoding human caveolin-1 and -2 are co-localized to the D7S522 locus (7q31.1), a known fragile site (FRA7G) that is frequently deleted in human cancers [J]. FEBS Lett, 436(3): 403-410.Fanzani A, Musaro A, Stoppani E, Giuliani R, Colombo F, Preti A, Marchesini S. 2007. Hypertrophy and atrophy inversely regulate caveolin-3 expression in myoblasts [J]. Biochem Biophys Res Commun, 357(1): 314-318.Hailstones D, Sleer LS, Parton RG, Stanley KK. 1998. Regulation of caveolin and caveolae by cholesterol in MDCK cells [J]. J Lipid Res, 39(2): 369-379.Jasmin JF, Malhotra S, Singh Dhallu M, Mercier I, Rosenbaum DM, Lisanti MP. 2007. Caveolin-1 deficiency increases cerebral ischemic injury [J]. Circ Res, 100(5): 721-729.Le Lay S, Blouin CM, Hajduch E, Dugail I. 2009. Filling up adipocytes with lipids: Lessons from caveolin-1 deficiency [J]. Biochim. Biophys Acta, 1791(6): 514-518.Lu X, Zhang Y, Wang X, Zhang Y, Xu J, Lai R, Gu Z. 2010. Identification and characterization of myostatin gene in Rough-skinned sculp, Trachidermus fasciatus[J]. Zool Res, 31(4): 387-394. [卢祥云,张  营,王星果,张燕萍,徐建荣,赖 仭,顾志良. 2010. 松江鲈(Trachidermus fasciatus肌肉生长抑制素基因克隆、序列和表达特征分析. 动物学研究,31(4): 387-394.])Parton RG, Hanzal-Bayer M, Hancock JF. 2006. Hancock. Biogenesis of caveolae: A structural model for caveolin-induced domain formation[J]. J Cell Sci, 119: 787-796.Pol A, Luetterforst R, Lindsay M, Heino S, Ikonen E, Parton RG. 2001. A caveolin dominant negative mutant associates with lipid bodies and induces intracellular cholesterol imbalance [J]. J Cell Biol, 152(5): 1057-1070.Pol A, Martin S, Fernandez MA, Ferguson C, Carozzi A, Luetterforst R, Enrich C, Parton RG. 2004. Dynamic and regulated association of caveolin with lipid bodies: modulation of lipid body motility and function by a dominant negative mutant [J]. Mol Biol cell, 15(1): 99-110.Scherer PE, Lewis RY, Volonte D, Engelman JA, Galbiati F, Couet J, Kohtz DS, van Donselaar E, Peters P, Lisanti MP. 1997. Cell-type and tissue-specific expression of caveolin-2: Caveolins 1 and 2 co-localize and form a stable hetero-oligomeric complex in vivo [J]. J Biol Chem, 272(46): 29337-29346.Smythe GM, Eby JC, Disatnik MH. 2003. A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization and activity and induces apoptosis in skeletal myotubes [J]. J Cell Sci, 116: 4739-4749., Rando TASonnino S, Prinetti A. 2009. Sphingolipids and membrane environments for caveolin [J]. FEBS Lett, 583(4): 597-606.Wang C, Mei YJ, Li L, Mo DL, Li JQ, Zhang H, Tian XG,Chen YS.2008. Molecular characterization and expression analysis of caveolin-1 in pig tissues
    [J]. Sci Chn Ser C: Life Sci, 51(7) : 655-661.Yamada E. 1955. The fine structures of the gall bladder epithelium of the mouse [J]. J Biophys Biochem Cytol,1(5): 445-458.Zhu Z, Li Y, Mo D, Li K, Zhao S. 2006. Molecular characterization and expression analysis of the porcine Caveolin-3 gene [J]. Biochem Biophys Res Commun, 346(1): 7-13.Cohen AW, Park DS, Woodman SE, Williams TM, Chandra M, Shirani J, Pereira de Souza A, Kitsis RN, Russell RG, Weiss LM, Tang B, Jelicks LA, Factor SM, Shtutin V, Tanowitz HB, Lisanti MP. 2003. Caveolin-1 null mice develop cardiac hypertrophy with hyperactivation of p42/44 MAP kinase in cardiac fibroblasts [J]. Am J Physiol Cell Physiol, 284(2): 457-474.Cohen AW, Razani B, Wang XB, Combs TP, Williams TM, Scherer PE, Lisanti MP. 2003. Caveolin-1-deficient mice show insulin resistance and defective insulin receptor protein expression in adipose tissue [J]. Am J Physiol Cell Physiol, 285(1): 222-235.Engelman JA, Zhang XL, Lisanti MP. 1998. Genes encoding human caveolin-1 and -2 are co-localized to the D7S522 locus (7q31.1), a known fragile site (FRA7G) that is frequently deleted in human cancers [J]. FEBS Lett, 436(3): 403-410.Fanzani A, Musaro A, Stoppani E, Giuliani R, Colombo F, Preti A, Marchesini S. 2007. Hypertrophy and atrophy inversely regulate caveolin-3 expression in myoblasts [J]. Biochem Biophys Res Commun, 357(1): 314-318.Hailstones D, Sleer LS, Parton RG, Stanley KK. 1998. Regulation of caveolin and caveolae by cholesterol in MDCK cells [J]. J Lipid Res, 39(2): 369-379.Jasmin JF, Malhotra S, Singh Dhallu M, Mercier I, Rosenbaum DM, Lisanti MP. 2007. Caveolin-1 deficiency increases cerebral ischemic injury [J]. Circ Res, 100(5): 721-729.Le Lay S, Blouin CM, Hajduch E, Dugail I. 2009. Filling up adipocytes with lipids: Lessons from caveolin-1 deficiency [J]. Biochim. Biophys Acta, 1791(6): 514-518.Lu X, Zhang Y, Wang X, Zhang Y, Xu J, Lai R, Gu Z. 2010. Identification and characterization of myostatin gene in Rough-skinned sculp, Trachidermus fasciatus[J]. Zool Res, 31(4): 387-394. [卢祥云,张  营,王星果,张燕萍,徐建荣,赖 仭,顾志良. 2010. 松江鲈(Trachidermus fasciatus肌肉生长抑制素基因克隆、序列和表达特征分析. 动物学研究,31(4): 387-394.])Parton RG, Hanzal-Bayer M, Hancock JF. 2006. Hancock. Biogenesis of caveolae: A structural model for caveolin-induced domain formation
    [J]. J Cell Sci, 119: 787-796.Pol A, Luetterforst R, Lindsay M, Heino S, Ikonen E, Parton RG. 2001. A caveolin dominant negative mutant associates with lipid bodies and induces intracellular cholesterol imbalance[J]. J Cell Biol, 152(5): 1057-1070.Pol A, Martin S, Fernandez MA, Ferguson C, Carozzi A, Luetterforst R, Enrich C, Parton RG. 2004. Dynamic and regulated association of caveolin with lipid bodies: modulation of lipid body motility and function by a dominant negative mutant[J]. Mol Biol cell, 15(1): 99-110.Scherer PE, Lewis RY, Volonte D, Engelman JA, Galbiati F, Couet J, Kohtz DS, van Donselaar E, Peters P, Lisanti MP. 1997. Cell-type and tissue-specific expression of caveolin-2: Caveolins 1 and 2 co-localize and form a stable hetero-oligomeric complex in vivo [J]. J Biol Chem, 272(46): 29337-29346.Smythe GM, Eby JC, Disatnik MH. 2003. A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization and activity and induces apoptosis in skeletal myotubes [J]. J Cell Sci, 116: 4739-4749., Rando TASonnino S, Prinetti A. 2009. Sphingolipids and membrane environments for caveolin [J]. FEBS Lett, 583(4): 597-606.Wang C, Mei YJ, Li L, Mo DL, Li JQ, Zhang H, Tian XG,Chen YS.2008. Molecular characterization and expression analysis of caveolin-1 in pig tissues
    [J]. Sci Chn Ser C: Life Sci, 51(7) : 655-661.Yamada E. 1955. The fine structures of the gall bladder epithelium of the mouse [J]. J Biophys Biochem Cytol,1(5): 445-458.Zhu Z, Li Y, Mo D, Li K, Zhao S. 2006. Molecular characterization and expression analysis of the porcine Caveolin-3 gene [J]. Biochem Biophys Res Commun, 346(1): 7-13. 
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Cloning and characterization of Caveolin-1 gene in pigeon,Columba livia domestica

doi: 10.3724/SP.J.1141.2010.05476
  • Author Bio:

  • Corresponding author: GU Zhi-Liang

Abstract: Caveolins, a class ofprincipal proteins forming the structure of caveolae in plasmalemma, were encoded by caveolins gene family. Caveolin-1 gene is a member of caveolins gene family. In the present study, a full-length of 2605 bp caveolin-1 cDNA sequence in Columba livia domestica, which included a 537 bp complete ORF encoding a 178 amino acids long putative peptide, were obtained by using RT-PCR and RACE technique. The Columba livia domestica caveolin-1CDS shared 80.1% − 93.4% homology with Bos taurus, Canis lupus familiaris, Gallus gallus and Rattus norvegicus. Meanwhile, the putative amino acid sequence of Columba livia domestica caveolin-1 shared 85.4% − 97.2% homology with the above species. The semi-quantity RT-PCR revealed that Caveolin-1expressions were detectable in all the Columba livia domestica tissues and the expressional level of caveolin-1 gene was high in adipose, medium in various muscles, low in liver. These results demonstrated that Caveolin-1 gene was potentially involved in some metabolic pathways in adipose and muscle.

ZHANG Ying, YU Jian-Feng, YANG Li, WANG Xing-Guo, GU Zhi-Liang. Cloning and characterization of Caveolin-1 gene in pigeon,Columba livia domestica. Zoological Research, 2010, 31(5): 476-482. doi: 10.3724/SP.J.1141.2010.05476
Citation: ZHANG Ying, YU Jian-Feng, YANG Li, WANG Xing-Guo, GU Zhi-Liang. Cloning and characterization of Caveolin-1 gene in pigeon,Columba livia domestica. Zoological Research, 2010, 31(5): 476-482. doi: 10.3724/SP.J.1141.2010.05476
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