Volume 31 Issue 5
Sep.  2010
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ZHAO Cai-Lian, YANG Qi-Wen, HU Jia-Rui, YE Ding, GONG Wu-Ming, Lü Hai-Ying, XU Z. Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish. Zoological Research, 2010, 31(5): 469-475. doi: 10.3724/SP.J.1141.2010.05469
Citation: ZHAO Cai-Lian, YANG Qi-Wen, HU Jia-Rui, YE Ding, GONG Wu-Ming, Lü Hai-Ying, XU Z. Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish. Zoological Research, 2010, 31(5): 469-475. doi: 10.3724/SP.J.1141.2010.05469

Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish

doi: 10.3724/SP.J.1141.2010.05469
Funds:  This investigation was supported by grants from the National Basic Research Program of China (2004CB117400); the National Natural Science Foundation of China (30150005; 30270675)
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  • Author Bio:

    ZHAO Cai-Lian,E-mail:cailian305@yahoo.com.cn

  • Received Date: 2010-03-15
  • Rev Recd Date: 2010-08-11
  • Publish Date: 2010-10-22
  • In an in silico search for gonand specific expressed genes, we have identified zRAP55 which is enriched in the ovary of zebrafish .zRAP55 encodes a protein of 382 amino acids with a highly conserved Lsm domain. zRAP55 protein shares more than 56% identities with that of other vertebrate species. RT-PCR results show that it is predominantly expressed in the ovary. In situ hybridization and immunohistochemistry studies reveal that zRAP55 is ubiquitously dispersed throughout the cytoplasm of stages Ⅰ and Ⅱ oocytes, whereas no expression is observed in stages Ⅲ and Ⅳ oocytes. As an RNA associated protein, zRAP55 might function in the control of protein translation at the early stages of oogenesis in zebrafish.
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Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish

doi: 10.3724/SP.J.1141.2010.05469
Funds:  This investigation was supported by grants from the National Basic Research Program of China (2004CB117400); the National Natural Science Foundation of China (30150005; 30270675)
  • Author Bio:

Abstract: In an in silico search for gonand specific expressed genes, we have identified zRAP55 which is enriched in the ovary of zebrafish .zRAP55 encodes a protein of 382 amino acids with a highly conserved Lsm domain. zRAP55 protein shares more than 56% identities with that of other vertebrate species. RT-PCR results show that it is predominantly expressed in the ovary. In situ hybridization and immunohistochemistry studies reveal that zRAP55 is ubiquitously dispersed throughout the cytoplasm of stages Ⅰ and Ⅱ oocytes, whereas no expression is observed in stages Ⅲ and Ⅳ oocytes. As an RNA associated protein, zRAP55 might function in the control of protein translation at the early stages of oogenesis in zebrafish.

ZHAO Cai-Lian, YANG Qi-Wen, HU Jia-Rui, YE Ding, GONG Wu-Ming, Lü Hai-Ying, XU Z. Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish. Zoological Research, 2010, 31(5): 469-475. doi: 10.3724/SP.J.1141.2010.05469
Citation: ZHAO Cai-Lian, YANG Qi-Wen, HU Jia-Rui, YE Ding, GONG Wu-Ming, Lü Hai-Ying, XU Z. Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish. Zoological Research, 2010, 31(5): 469-475. doi: 10.3724/SP.J.1141.2010.05469
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