Volume 30 Issue 5
Sep.  2009
Turn off MathJax
Article Contents

JIN Li-fang, JI Shao-hui, GUO Xiang-yu, WANG Xi-hong, JI Wei-zhi. Induction of Rhesus Monkey Embryonic Stem Cells into Hepatocyte-like Cells by a Three-step Method. Zoological Research, 2009, 30(5): 509-514. doi: 10.3724/SP.J.1141.2009.05509
Citation: JIN Li-fang, JI Shao-hui, GUO Xiang-yu, WANG Xi-hong, JI Wei-zhi. Induction of Rhesus Monkey Embryonic Stem Cells into Hepatocyte-like Cells by a Three-step Method. Zoological Research, 2009, 30(5): 509-514. doi: 10.3724/SP.J.1141.2009.05509

Induction of Rhesus Monkey Embryonic Stem Cells into Hepatocyte-like Cells by a Three-step Method

doi: 10.3724/SP.J.1141.2009.05509
More Information
  • Corresponding author: JI Wei-zhi
  • Received Date: 2009-04-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2009-10-22
  • Embryonic stem cells (ESCs) hold great potential for the treatment of liver diseases. Here, we reported the induction of rhesus monkey ESCs along a hepatocyte lineage by a three-step method. The Rhesus monkey ESCs were suspended in DMEM medium supplemented with 10% FBS for 11 days to form embryonic bodies (EB) which contained early endoderm or hepatic cells by expression of early hepatic genes. EB of 11 days were transferred into ECM coated dishes and cultured with aFGF, BMP-4 and OSM at different time points for further studies. After induction by aFGF and BMP-4 for 7 - 10 days, the differentiated cells showed a hepatocyte-like morphology, expressed hepatic marker genes (such as AFP,ALB APOH,G-6-P and TAT) and proteins (AFP,ALB and CK18), and possessed the capacity to storage intracytoplasmic glycogen. After further induction with OSM for 7 - 10 days, some cells shared similar characteristics of matured hepatocytes, such as expression of genes of CYP1B1 and ADH1C, and ICG uptaken, indicating the presence of functional hepatocytes in the differentiated cells.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1759) PDF downloads(1740) Cited by()

Related
Proportional views

Induction of Rhesus Monkey Embryonic Stem Cells into Hepatocyte-like Cells by a Three-step Method

doi: 10.3724/SP.J.1141.2009.05509
    Corresponding author: JI Wei-zhi

Abstract: Embryonic stem cells (ESCs) hold great potential for the treatment of liver diseases. Here, we reported the induction of rhesus monkey ESCs along a hepatocyte lineage by a three-step method. The Rhesus monkey ESCs were suspended in DMEM medium supplemented with 10% FBS for 11 days to form embryonic bodies (EB) which contained early endoderm or hepatic cells by expression of early hepatic genes. EB of 11 days were transferred into ECM coated dishes and cultured with aFGF, BMP-4 and OSM at different time points for further studies. After induction by aFGF and BMP-4 for 7 - 10 days, the differentiated cells showed a hepatocyte-like morphology, expressed hepatic marker genes (such as AFP,ALB APOH,G-6-P and TAT) and proteins (AFP,ALB and CK18), and possessed the capacity to storage intracytoplasmic glycogen. After further induction with OSM for 7 - 10 days, some cells shared similar characteristics of matured hepatocytes, such as expression of genes of CYP1B1 and ADH1C, and ICG uptaken, indicating the presence of functional hepatocytes in the differentiated cells.

JIN Li-fang, JI Shao-hui, GUO Xiang-yu, WANG Xi-hong, JI Wei-zhi. Induction of Rhesus Monkey Embryonic Stem Cells into Hepatocyte-like Cells by a Three-step Method. Zoological Research, 2009, 30(5): 509-514. doi: 10.3724/SP.J.1141.2009.05509
Citation: JIN Li-fang, JI Shao-hui, GUO Xiang-yu, WANG Xi-hong, JI Wei-zhi. Induction of Rhesus Monkey Embryonic Stem Cells into Hepatocyte-like Cells by a Three-step Method. Zoological Research, 2009, 30(5): 509-514. doi: 10.3724/SP.J.1141.2009.05509

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return