Volume 29 Issue 5
Sep.  2008
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LIU Shu-bai, HE Ying-ying, QIAN Jinqiao, ZHANG Yun. CAT, Induced Isolated Rabbit Thoracic Aortic Rings Sustained Contraction in Endothelial-dependent Manner. Zoological Research, 2008, 29(5): 493-502. doi: 10.3724/SP.J.1141.2008.05493
Citation: LIU Shu-bai, HE Ying-ying, QIAN Jinqiao, ZHANG Yun. CAT, Induced Isolated Rabbit Thoracic Aortic Rings Sustained Contraction in Endothelial-dependent Manner
. Zoological Research, 2008, 29(5): 493-502. doi: 10.3724/SP.J.1141.2008.05493

CAT, Induced Isolated Rabbit Thoracic Aortic Rings Sustained Contraction in Endothelial-dependent Manner

doi: 10.3724/SP.J.1141.2008.05493
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  • Corresponding author: ZHANG Yun
  • Received Date: 2008-06-16
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2008-10-22
  • In vertebrates, non-lens bg-crystallins are widely expressed in various tissues and their functions are not well known. The molecular mechanisms of trefoil factors (TFFs), which involved in mucosal healing and tumorigenesis, have remained elusive. bg-CAT is a novel multifunctional protein complex of non-lens bg-crystallin and trefoil factor from frog skin secretions. Here we report that bg-CAT could induce sustained contraction of isolated rabbit aortic rings in dosage (2-35 nmol/L) and endothelium dependent manners (P<0.01). In addition, in situ immunofluorescence indicated that positive TNF-a signals were mainly detected at the endothelial cell layer of bg-CAT (25 nmol/L) treated rings. Furthermore,bg-CAT induced primary cultured rabbit thoracic aortic endothelial cells (RAECs) rapidly to release TNF-a. After bg-CAT (25 nmol/L) treated for 10 and 30 min, the levels of the endothelial cells released TNF-a were 34.17 Keywords:
  • a')"> Tumor necrosis factor-a
  • Trefoil factor
  • Non-lens bg-crystallin
  • Endothelium-dependent aorta vasoconstriction
  • bg-CAT
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  • 通讯作者: 陈斌, bchen63@163.com
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      沈阳化工大学材料科学与工程学院 沈阳 110142

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    CAT, Induced Isolated Rabbit Thoracic Aortic Rings Sustained Contraction in Endothelial-dependent Manner

    doi: 10.3724/SP.J.1141.2008.05493
      Corresponding author: ZHANG Yun

    Abstract: In vertebrates, non-lens bg-crystallins are widely expressed in various tissues and their functions are not well known. The molecular mechanisms of trefoil factors (TFFs), which involved in mucosal healing and tumorigenesis, have remained elusive. bg-CAT is a novel multifunctional protein complex of non-lens bg-crystallin and trefoil factor from frog skin secretions. Here we report that bg-CAT could induce sustained contraction of isolated rabbit aortic rings in dosage (2-35 nmol/L) and endothelium dependent manners (P<0.01). In addition, in situ immunofluorescence indicated that positive TNF-a signals were mainly detected at the endothelial cell layer of bg-CAT (25 nmol/L) treated rings. Furthermore,bg-CAT induced primary cultured rabbit thoracic aortic endothelial cells (RAECs) rapidly to release TNF-a. After bg-CAT (25 nmol/L) treated for 10 and 30 min, the levels of the endothelial cells released TNF-a were 34.17

    LIU Shu-bai, HE Ying-ying, QIAN Jinqiao, ZHANG Yun. CAT, Induced Isolated Rabbit Thoracic Aortic Rings Sustained Contraction in Endothelial-dependent Manner. Zoological Research, 2008, 29(5): 493-502. doi: 10.3724/SP.J.1141.2008.05493
    Citation: LIU Shu-bai, HE Ying-ying, QIAN Jinqiao, ZHANG Yun. CAT, Induced Isolated Rabbit Thoracic Aortic Rings Sustained Contraction in Endothelial-dependent Manner
    . Zoological Research, 2008, 29(5): 493-502. doi: 10.3724/SP.J.1141.2008.05493

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