Volume 29 Issue 4
Jul.  2008
Turn off MathJax
Article Contents
ZHOU Hui-fang, LU Xiao-peng, SUN Zheng-hua, GUO Yan, ZHANG Hua-tang. Establishment and Characterization of THP-1 Cell Model Stably Expressing HIV-1 Auxiliary Protein Rev. Zoological Research, 2008, 29(4): 421-426. doi: 10.3724/SP.J.1141.2008.04421
Citation: ZHOU Hui-fang, LU Xiao-peng, SUN Zheng-hua, GUO Yan, ZHANG Hua-tang. Establishment and Characterization of THP-1 Cell Model Stably Expressing HIV-1 Auxiliary Protein Rev. Zoological Research, 2008, 29(4): 421-426. doi: 10.3724/SP.J.1141.2008.04421

Establishment and Characterization of THP-1 Cell Model Stably Expressing HIV-1 Auxiliary Protein Rev

doi: 10.3724/SP.J.1141.2008.04421
More Information
  • Corresponding author: ZHANG Hua-tang
  • Received Date: 2008-04-08
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2008-08-22
  • HIV-1 auxiliary proteins play a critical role in both the infection of HIV-1 target cells and the morbidity of AIDS. Rev, as one of the HIV-1 auxiliary proteins, is essential for the replication of HIV-1. As a membrane-shuttle protein, it can regulate the transportation of HIV-1 structural protein’s mRNA. The rev gene from HIV-1 was transfected into THP-1 cells. The Rev-stably-expressed cell model was generated by electroporation and then sorted and selected with G418. The cell model was characterized at both mRNA and protein levels with an RT-PCR assay, fluorescent observation, and flow cytometry analysis. The rev gene was successfully transfected into THP-1 cells and stably expressed. This is a useful result for future research of the interaction between Rev and cells.
  • loading
  • Relative Articles

    [1] Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 2016, 37(2): 96-102.  doi: 10.13918/j.issn.2095-8137.2016.2.96
    [2] Lin ZHANG, Xu-He HUANG, Ping-Ping ZHOU, Guo-Long YU, Jin YAN, Bing QIN, Xin-Ge YAN, Li-Mei DIAO, Peng LIN, Yi-Qun KUANG. Establishment of HIV-1 model cell line GHOST(3) with stable DRiP78 and NHERF1 knockdown. Zoological Research, 2015, 36(3): 161-166.
    [3] Jin-Hua WANG, Liang MIAO, Ming-Yun LI, Xiao-Fei GUO, Na PAN, Ying-Ying CHEN, Liang ZHAO. Cloning the Dmrt1 and DmrtA2 genes of ayu (Plecoglossus altivelis) and mapping their expression in adult, larval, and embryonic stages. Zoological Research, 2014, 35(2): 99-107.  doi: 10.11813/j.issn.0254-5853.2014.2.099
    [4] Kou PENG, Cheng-Yuan WANG, Jun-Hua WANG, Jun-Qing SHENG, Jian-Wu SHI, Jian LI, Yi-Jiang HONG. Molecular cloning, sequence analysis, and cadmium stress-rated expression changes of BTG1 in freshwater pearl mussel (Hyriopsis schlegelii). Zoological Research, 2014, 35(5): 389-397.  doi: 10.13918/j.issn.2095-8137.2014.5.389
    [5] SONG Hai-Yan, LIU Zai-Qun, ZHENG Lei. Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose. Zoological Research, 2012, (2): 211-217.  doi: 10.3724/SP.J.1141.2012.02211
    [6] YANG Ming-Hua, SU Jin-Zhu, LIU Jun, LI Ya-Hui. Cloning and expression of Tssk1 & Tssk2 in mice and the presence & localization of them in mature sperm. Zoological Research, 2012, 33(4): 381-388.  doi: 10.3724/SP.J.1141.2012.04381
    [7] Jinling CAO, Jianjie CHEN, Zhongliang JIANG, Yongju LUO, Xi GAN. Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus. Zoological Research, 2012, 33(E3-4): 40-46.  doi: 10.3724/SP.J.1141.2012.E03-04E40
    [8] WANG Zi-Sheng, QI Zhi-Tao, TIAN Jing-Yun, QIU Ming, ZHAO Wei-Hong, WANG Ai-Min, HUA. Cloning of hemoglobin-α1 from half-smooth tongue sole (Cynoglossus semilaevis) and its expression under short-term hypoxia. Zoological Research, 2011, 32(6): 641-646.  doi: 10.3724/SP.J.1141.2011.06641
    [9] FAN Wu-Jiang, LI Si-Fa. cDNA cloning and tissue-differential expression of Na+/K+/2Cl- −cotransporter 1-α isoform in Sarotherodon melanotheron. Zoological Research, 2010, 31(6): 601-609.  doi: 10.3724/SP.J.1141.2010.06601
    [10] ZHANG Ying, JIN Shan, ZHAO Qing-Song, WANG Guo-Liang, YU Kai, WANG Chun-Lin. Cloning and expression pattern analysis of a lipopolysaccharide -and β-1,3-glucan-binding protein in Portunus trituberculatus. Zoological Research, 2010, 31(6): 587-594.  doi: 10.3724/SP.J.1141.2010.06587
    [11] YANG Yue-qin, YI Xian-feng, LI Ning. Analysis on Trophic Structure of Main Waterbirds in Qinghai Lake Based on Stable Isotopic Technology. Zoological Research, 2009, 30(4): 418-422.  doi: 10.3724/SP.J.1141.2009.04418
    [12] DENG Si-ping, CHEN Song-lin, LIU Ben-wei. Molecular Cloning and Expression Analysis of FTZ-F1 in the Half-smooth Tongue-sole, Cynoglossus semilaevis. Zoological Research, 2008, 29(6): 592-598.  doi: 10.3724/SP.J.1141.2008.06592
    [13] MA Li, ZHAO Shu-hua, KONG Qing-hua, MAO Bing-yu, *. Temporal and Spatial Expression Patterns of Sox1 Gene in Xenopus laevis Embryo. Zoological Research, 2007, 28(4): 403-408.
    [14] MA Dong-mei, ZHU Hua-ping, GUI Jian-fang. Molecular Cloning and Expression Characterization of Prox1 in Goldfish (Carassius auratus) Eye Development. Zoological Research, 2007, 28(6): 589-596.
    [15] DONG Shuang-lin HAN Zhao-jun Martin S WILLIAMSON , *. Expression of the Aphis gossypii AChE1 Gene Using the Baculovirus Expression System. Zoological Research, 2006, 27(1): 75-80.
    [16] YI Xian-feng. Food Resource Partitioning in Alpine Weasel,Steppe Polecat and Upland Buzzard:Evidence from Stable Isotope Ratios. Zoological Research, 2005, 26(1): 1-7.
    [17] YI Xian-feng, ZHANG Xiao-ai, LI Lai-xing, LI Ming-cai ZHAO Liang. Analysis on Food Web Structure in Alpine Meadow Ecosystem:Evidence from Stable Carbon Isotope Signatures. Zoological Research, 2004, 25(1): 1-6.
    [18] YI Xian-feng, LI Lai-xing, ZHANG Xiao-ai, ZHAO Liang, LI Ming-cai. Influence of Artificial Food on Stable Carbon and Nitrogen Isotope Composition of Plateau Pikas. Zoological Research, 2004, 25(3): 232-235.
    [19] ZHAO Liang, YI Xian-feng, ZHOU Hua-kun, ZHANG Xiao-ai. Model of Trophic Levels of Animals in Alpine Meadow Ecosystem by Using Stable Isotopes. Zoological Research, 2004, 25(6): 497-503.
    [20] ZHAO Tong-biao, ZHAO Xin-quan, CHANG Zhi-jie, SUN Ping, XU Shi-xiao, ZHAO Wei. Tissue Specific Expression of Plateau Pikas (Ochotona curzoniae) HIF-1α mRNA Under Normal Oxygen. Zoological Research, 2004, 25(2): 132-136.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1977) PDF downloads(3012) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint