Volume 29 Issue 4
Jul.  2008
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ZHOU Hui-fang, LU Xiao-peng, SUN Zheng-hua, GUO Yan, ZHANG Hua-tang. Establishment and Characterization of THP-1 Cell Model Stably Expressing HIV-1 Auxiliary Protein Rev. Zoological Research, 2008, 29(4): 421-426. doi: 10.3724/SP.J.1141.2008.04421
Citation: ZHOU Hui-fang, LU Xiao-peng, SUN Zheng-hua, GUO Yan, ZHANG Hua-tang. Establishment and Characterization of THP-1 Cell Model Stably Expressing HIV-1 Auxiliary Protein Rev. Zoological Research, 2008, 29(4): 421-426. doi: 10.3724/SP.J.1141.2008.04421

Establishment and Characterization of THP-1 Cell Model Stably Expressing HIV-1 Auxiliary Protein Rev

doi: 10.3724/SP.J.1141.2008.04421
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  • Corresponding author: ZHANG Hua-tang
  • Received Date: 2008-04-08
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2008-08-22
  • HIV-1 auxiliary proteins play a critical role in both the infection of HIV-1 target cells and the morbidity of AIDS. Rev, as one of the HIV-1 auxiliary proteins, is essential for the replication of HIV-1. As a membrane-shuttle protein, it can regulate the transportation of HIV-1 structural protein’s mRNA. The rev gene from HIV-1 was transfected into THP-1 cells. The Rev-stably-expressed cell model was generated by electroporation and then sorted and selected with G418. The cell model was characterized at both mRNA and protein levels with an RT-PCR assay, fluorescent observation, and flow cytometry analysis. The rev gene was successfully transfected into THP-1 cells and stably expressed. This is a useful result for future research of the interaction between Rev and cells.
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