Volume 29 Issue 4
Jul.  2008
Turn off MathJax
Article Contents
ZHANG Jing, WEI Qiang, LU Bin, CHEN Yong-chang, CHEN Hong-wei, LI Rong-rong. Influence of Different FGF2-expressing Feeder Layers on the Self-renewal and Pluripotency of Rhesus Monkey Embryonic Stem Cells. Zoological Research, 2008, 29(4): 405-414. doi: 10.3724/SP.J.1141.2008.04405
Citation: ZHANG Jing, WEI Qiang, LU Bin, CHEN Yong-chang, CHEN Hong-wei, LI Rong-rong. Influence of Different FGF2-expressing Feeder Layers on the Self-renewal and Pluripotency of Rhesus Monkey Embryonic Stem Cells. Zoological Research, 2008, 29(4): 405-414. doi: 10.3724/SP.J.1141.2008.04405

Influence of Different FGF2-expressing Feeder Layers on the Self-renewal and Pluripotency of Rhesus Monkey Embryonic Stem Cells

doi: 10.3724/SP.J.1141.2008.04405
  • Received Date: 2008-04-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2008-08-22
  • Transgene and RNAi were used to establish five different FGF2-expressing monkey ear skin fibroblast (MESF) cell lines, the FGF2 over-expressed line (f1), the negative control of f1 (f2), RNA interfered line (f3), the negative control of f3 (f4) and the non-treated control (f5). The expression ratio of FGF2 in these lines was f1: f2: f3: f4: f5=4∶2∶1∶2∶2. The results indicated that c-fos, TGF-β1, INHBA, Gremlin1 were upregulated in f1 but downregulated in f3, while BMP4, TGF-β2 were downregulated in f1 but upregulated in f3, which implied that endogenous FGF2 affected the TGF-β signaling pathway and the expression level of related genes changed in MESFs. Further analysis of rhesus monkey embryonic stem cells (RhESCs) supported by these MESFs showed that RhESCs on f1 grew more quickly than those in other groups, in which the expression level of c-fos,TGF-β1,INHBA,Gremlin1,OCT-4,Nanog and Sox2 was higher, while that of BMP4 was lower; in contrast, RhESCs on f3 grew more slowly than in other groups, in which the expression level of c-fos,TGF-β1,INHBA,Gremlin1,OCT-4,Nanog and Sox2 declined, while that of BMP4 and TGF-β2 increased. EBs from these RhESCs expressed early markers representing all germ layers, but the expression levels of markers in RhESCs on f1 were lower than in other groups. These findings demonstrated that different FGF2 expression in feeder layers can not only influence expression of relative genes in MESFs, but also affect proliferation and self-renewal of ESCs.
  • loading
  • Relative Articles

    [1] John P. Capitanio, Frederik Dethloff, Christoph W. Turck. Lipid metabolism is associated with temperament, corticosteroid, and hematological measures in infant rhesus monkeys (Macaca mulatta). Zoological Research, 2020, 41(6): 709-714.  doi: 10.24272/j.issn.2095-8137.2020.244
    [2] Zhen-Hui Li, Xia-Ping He, Hao Li, Rong-Qiao He, Xin-Tian Hu. Age-associated changes in amyloid-β and formaldehyde concentrations in cerebrospinal fluid of rhesus monkeys. Zoological Research, 2020, 41(4): 444-448.  doi: 10.24272/j.issn.2095-8137.2020.088
    [3] Shi-Hao Wu, Zhi-Xing Liao, Joshua D. Rizak, Na Zheng, Lin-Heng Zhang, Hen Tang, Xiao-Bin He, Yang Wu, Xia-Ping He, Mei-Feng Yang, Zheng-Hui Li, Dong-Dong Qin, Xin-Tian Hu. Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains. Zoological Research, 2017, 38(2): 88-95.  doi: 10.24272/j.issn.2095-8137.2017.015
    [4] TANG Xiang-Hui, CAO Yue-Ling, YANG Ze-Xing, ZHAO Fu-Xian. Reproductive traits of polycystic ovary syndrome in female rhesus monkeys. Zoological Research, 2012, 33(1): 37-42.  doi: 10.3724/SP.J.1141.2012.01037
    [5] BIAN Hui, FAN Yao-Dong, GUO Li-Yun, YU Hua-Lin. A technique of rhesus monkey neural progenitor cells intravitreal transplant to rats. Zoological Research, 2012, 33(1): 85-88.  doi: 10.3724/SP.J.1141.2012.01085
    [6] DONG Jin-Run, GUO Li-Yun, QU Jia-Gui, QI Ren-Li, WANG Wen-Chao, XIAO Chun-Jie, WANG Zheng-Bo. Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation. Zoological Research, 2012, 33(1): 43-48.  doi: 10.3724/SP.J.1141.2012.01043
    [7] GUO Xiang-Yu, JIN Li-Fang, JI Shao-Hui, JI Wei-Zhi. Rhesus monkey (Macaca mulatta) muller cells exhibit retinal stem/progenitor cell features in vitro. Zoological Research, 2011, 32(6): 611-616.  doi: 10.3724/SP.J.1141.2011.06616
    [8] JIN Li-Fang, JI Shao-Hui, YANG Ji-Feng, JI Wei-Zhi. Notch signaling dependent differentiation of cholangiocyte-likecells from rhesus monkey embryonic stem cells. Zoological Research, 2011, 32(4): 391-395.  doi: 10.3724/SP.J.1141.2011.04391
    [9] , , , , , , . Tissue Distribution of TRIM5α in Rhesus Monkey and Upregulation in Peripheral Blood Mononuclear Cell by Using Different Stimuli. Zoological Research, 2009, 30(4): 354-360.  doi: 10.3724/SP.J.1141.2009.04354
    [10] CHEN Dong-liang, LI Rong-rong, ZHANG Jing, LU Bin, WEI Qiang, WANG Shu-fen, XIE Yun-h. Comparative Analysis of Five Different Homologous Feeder Cell Lines in the Ability to Support Rhesus Embryonic Stem Cells. Zoological Research, 2009, 30(4): 345-353.  doi: 10.3724/SP.J.1141.2009.04345
    [11] JIN Li-fang, JI Shao-hui, GUO Xiang-yu, WANG Xi-hong, JI Wei-zhi. Induction of Rhesus Monkey Embryonic Stem Cells into Hepatocyte-like Cells by a Three-step Method. Zoological Research, 2009, 30(5): 509-514.  doi: 10.3724/SP.J.1141.2009.05509
    [12] LI Rong-rong, CHEN Hong-wei, CHEN Dong-liang, WANG Shu-fen, ZHAN jing, CHEN Rui, J. Hepatocyte Growth Factor Promotes the Proliferation of the Neural Progenitors Derived from Rhesus Monkey Embryonic Stem Cells. Zoological Research, 2008, 29(5): 518-528.  doi: 10.3724/SP.J.1141.2008.05518
    [13] QU Jia-gui, WANG Zheng-bo, DONG Jin-run, MA Yuan-ye. Inhibitory Effect of Fluorocitrate on the Proliferation of Cultured Glioblastomas G422 Cells. Zoological Research, 2008, 29(4): 427-430.  doi: 10.3724/SP.J.1141.2008.04427
    [14] LI Yi , HUANG Wei, ZHANG Xin, SU Bing , *. Construction of a cDNA Library of the Prefrontal Cortex of Rhesus Monkey. Zoological Research, 2006, 27(3): 325-330.
    [15] CAI Ke-jun, LI Ya-hui, LI Jian, HE Xie-chao, JI Wei-zhi. Effects of Extenders Varying in Osmolality on Rhesus Monkey (Macaca mulatta) Spermatozoa Cryopreservation. Zoological Research, 2005, 26(3): 305-310.
    [16] YU Hua-lin, LIU Yan-cheng, FAN Yao-dong, MA Yi-liu, FENG Zhong-tang. Effect of Morphine on γ-band of EEG Activity in the Orbital Frontal Cortex of Rhesus Monkey. Zoological Research, 2005, 26(4): 442-445.
    [17] PEI Yi-jin, JI Wei-zhi. Induced Differentiation and Apoptosis of Rhesus Monkey Embryonic Stem Cells. Zoological Research, 2003, 24(3): 161-167.
    [18] ZHENG Ping, ZOU Rou-jin, LIU Yi-xun. Possible Involvement of Plasminogen Activator in the Acquirement of Sperm Progressive Motility and the Regulation of PA and PAI-1 mRNA Expressions in Epididymis,Prostate and Seminal Vesical in Rhesus Monkey. Zoological Research, 2002, 23(1): 19-24.
    [19] YANG Sen-fu, LI Shao-dong, DU Jin-you, DUAN Xing-shen. The Breeding Study on Laboratory Rhesus Monkeys. Zoological Research, 1989, 10(zk): 53-59.
    [20] WANG Li-de, WU Jun, DUAN Xing-sheng. The Investigation on Parasites in Rhesus Monkey From Wild-Caught in South China. Zoological Research, 1989, 10(zk): 34-60.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2711) PDF downloads(2917) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return