Volume 29 Issue 4
Jul.  2008
Turn off MathJax
Article Contents
CHEN Xia, YU Xiao-dong, DENG Min, LI Hui, LIN Yi-xin, HE Qi-yi, LIU Jian-ping. Purification and Characterization of 5′-nucleotidase from Trimeresurus albolabris Venom. Zoological Research, 2008, 29(4): 399-404. doi: 10.3724/SP.J.1141.2008.04399
Citation: CHEN Xia, YU Xiao-dong, DENG Min, LI Hui, LIN Yi-xin, HE Qi-yi, LIU Jian-ping. Purification and Characterization of 5′-nucleotidase from Trimeresurus albolabris Venom. Zoological Research, 2008, 29(4): 399-404. doi: 10.3724/SP.J.1141.2008.04399

Purification and Characterization of 5′-nucleotidase from Trimeresurus albolabris Venom

doi: 10.3724/SP.J.1141.2008.04399
More Information
  • Corresponding author: YU Xiao-dong
  • Received Date: 2008-01-30
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2008-08-22
  • A 5′-nucleotidase was isolated and purified from the snake venom of T. albolabris using three steps of chromatography including DEAE-SephadexA-25, Sephadex-G-100 and CM-Sephadex C-50. Using SDS-PAGE and HPLC column chromatography the purified 5′-nucleotidase proved to be homogenous. It was a glycoprotein with a molecular weight of 48.03 kDa. The enzymatic activities of the purified 5′-nucleotidase were 330.33 µg Pi/min mg and 123.56 µg Pi/min mg when using AMP(adenosine monophosphate) and ADP(adenosine diphosphate) as substrates, respectively. Metal ions, including Zn2+, Fe3+ and Cu2+, could inhibit 5′-nucleotidase activity, as did EDTA. Its optimum pH was nine and its optimum temperature was 50°C. It has a potent inhibitory effect on rabbit platelet aggregation induced by ADP.
  • loading
  • Relative Articles

    [1] LI Rui, WEI Qin, WANG Wan-yu, XIONG Yu-liang. Cloning of cDNA Encoding a C-type Lectin from Snake Trimeresurus jerdonii. Zoological Research, 2003, 24(4): 293-296.
    [2] LEE Wen-hui, ZHANG Yun. Research Development of Snake Venom C-type Lectins. Zoological Research, 2003, 24(2): 151-160.
    [3] LEE Wen-hui, GAO Rong, ZHANG Yun. Molecular Cloning and Sequence Comparison of Serine Proteases from the Venom of Trimeresurus stejnegeri. Zoological Research, 2003, 24(3): 180-185.
    [4] HANG Yun, XIONG Yu-liang, WANG Wan-yu, LI Wen-hui, ZHU Shao-wen. Snake Venom Serine Protease Diversity--Substrate Spectificity Immuno-Chemistry and Sequence Comparison Studies. Zoological Research, 1994, 15(3): 73-80.
    [5] XIONG Yu-liang, WANG Wan-yu, YANG Chang-jiu, YOU Fu-sheng. The Analysis of Snake Venom Output and The Concerned Factor. Zoological Research, 1992, 13(1): 73-76.
    [6] GE Bing, YU Hui-qun, WANG Jing-ying, XU Yan. A Sandwich Enzyme-Immunoassay For Detecting Snake Venom. Zoological Research, 1991, 12(1): 79-83.
    [7] ZHANG Yun, XIONG Yu-liang. Purification and Characterization of A New Fibrinogenase From The Venom of the Chinese Habu Snake (Trimeresurus mucrosquamatus). Zoological Research, 1991, 12(2): 199-207.
    [8] ZHANG Yun, XIONG Yu-liang. Effect of Purified Fibrinogenase From the Venom of The Chinese Habu Snake (Trimeresurus mucrosquamatus) on Fibrinogen and Thrombin. Zoological Research, 1991, 12(3): 277-283.
    [9] LIU Nian-kun, XIONG Yu-liang. Purification and Some Properties of the Thrombinlike Enzymes From Trimeresurus Stejnegeri Venom. Zoological Research, 1990, 11(1): 87-93.
    [10] XU Li-na, WANG Jian-ping, LIU Qin-yue. The Effects of Sodium Ferulate,Tetramethylpyrazine,Prostaglandin E1 and Prostacyclin on Platelet Aggregation Induced by the Venom of Trimeresurus mucrosquamatus in Rats. Zoological Research, 1987, 8(zk): 39-45.
    [11] CHEN Hong, WAN Hai-ying, RUAN Chang-geng. The Activation of Human Platelets Induced by the Aggregoserpentin of Trimeresurus mucrosquamatus Venom (TMVA). Zoological Research, 1987, 8(zk): 9-16.
    [12] WANG Wan-yu, YANG Chang-jiu et al., WU Xiang-fu, LIN Nan-qin et al.. Studies on Biochemical Properties of the Platelet Aggregation Inducer From Trimeresurus mucrosquamatus Venom. Zoological Research, 1987, 8(zk): 1-8.
    [13] WANG Xin-ming, QI Zheng-wu. Characterization of the Kinin Released by Kallikrein Ⅰ From Snake Venom (Agkistrodon halys Pallas). Zoological Research, 1983, 4(4): 321-326.
    [14] WANG Wan-yu, YANG Chang-jiu, XIONG Yu-liang, CHEN Xi-lan. Study on the Venom of Trimeresurus mucrosquamatus Ⅰ.Isolation of T.mucrosquamatus Venom and the Determination of its Biological and Enzymatic Activities. Zoological Research, 1983, 4(4): 327-332.
    [15] RUAN Chang-geng et al., GU Zhen-lun et al., WANG Wan-yu et al.. The Activation of Platelet Induced by the Venom of Trimeresurus Mucrosquamatus (From Hunan Province). Zoological Research, 1983, 4(3): 245-254.
    [16] HAO Wen-xue et al., HAN Tian-xiang et al., ZHANG Xi-yao et al.. Observation of 50 Cases on the Clinical Study of Snake Venom Arginine Esterase From the Snake in Snake Island Used in the Ischemic Cerebrovascular Diseases. Zoological Research, 1981, 2(zk): 113-116.
    [17] ZHANG Gon-jin. Study on Ribonuclease From Snake Venom of Zhejiang Cobra (Naja naja atra). Zoological Research, 1981, 2(zk): 103-111.
    [18] ZHANG Mu-sheng, HONG Geng-xin, HONG Xi-jun et al.. Anti-Snake-Venom and Pharmacological Effects of. Zoological Research, 1981, 2(zk): 131-137.
    [19] HAO Wen-xue, ZOU Ping-gong, HAN Tian-xiang. Studies on Anticoaculating Effect of Arginine Esterase From the Snake Venom of Snake Island. Zoological Research, 1981, 2(zk): 157-159.
    [20] WEI Qi, ZHOU De-yi, XU Qi-yu. Adpase,Atpase and 5' Nucleotidase of the Snake Venoms From Guangxi. Zoological Research, 1981, 2(zk): 143-146.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2389) PDF downloads(2193) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint