Volume 29 Issue 2
Mar.  2008
Turn off MathJax
Article Contents
ZHAO Hui, LIU Xiao-dong, GAO Zhen-hua, ZHANG Jian-hua, LI Hong, ZHANG Yun, LEE. Expression of Human Semenogelin I-52 and Antibacterial Activity Investigation of Recombinant Peptide. Zoological Research, 2008, 29(2): 139-144. doi: 10.3724/SP.J.1141.2008.02139
Citation: ZHAO Hui, LIU Xiao-dong, GAO Zhen-hua, ZHANG Jian-hua, LI Hong, ZHANG Yun, LEE. Expression of Human Semenogelin I-52 and Antibacterial Activity Investigation of Recombinant Peptide. Zoological Research, 2008, 29(2): 139-144. doi: 10.3724/SP.J.1141.2008.02139

Expression of Human Semenogelin I-52 and Antibacterial Activity Investigation of Recombinant Peptide

doi: 10.3724/SP.J.1141.2008.02139
  • Received Date: 2008-02-03
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2008-04-22
  • Nucleotide sequence coding for SgI-52 with amino acid residues of 85-136 form mature human semenogelin I was amplified by PCR technique from the cDNA of a human seminal vesicle. The obtained PCR products were inserted into vector pMAL-p2X. The constructed expression vector of pMAL-p2X/SgI-52 was transformed into Escherichia coli DH5α. Fusion protein was expressed in the periplasma of the E. coli DH5αafter IPTG inducement. Recombinant SgI-52 was purified after factor X cleavage and a ultrafiltering process. MALDI-TOF- MS results indicated that the purified recombinant SgI-52 was the target peptide. Recombinant SgI-52 showed antibacterial activities on E. coli ATCC 25922 and E. coli ML-35P with MIC values of 32.45 and 46.30 μg/mL, respectively. Our results and other relevant works suggested that different human semenogelin I degradation fragments during the liquefaction might have various biological functions and deserve to be investigated further.
  • loading
  • Relative Articles

    [1] Chao-Chao Yan, Xin-Shang Zhang, Liang Zhou, Qiao Yang, Min Zhou, Lin-Wan Zhang, Jin-Chuan Xing, Zhi-Feng Yan, Megan Price, Jing Li, Bi-Song Yue, Zhen-Xin Fan. Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humans. Zoological Research, 2020, 41(5): 557-563.  doi: 10.24272/j.issn.2095-8137.2020.092
    [2] Guang-Qi GAO, Li-Shuang SONG, Bin TONG, Guang-Peng LI. Expression levels of GSTA2 and APOD genes might be associated with carotenoid coloration in golden pheasant (Chrysolophus pictus) plumage. Zoological Research, 2016, 37(3): 144-150.  doi: 10.13918/j.issn.2095-8137.2016.3.144
    [3] Jin-Hua WANG, Liang MIAO, Ming-Yun LI, Xiao-Fei GUO, Na PAN, Ying-Ying CHEN, Liang ZHAO. Cloning the Dmrt1 and DmrtA2 genes of ayu (Plecoglossus altivelis) and mapping their expression in adult, larval, and embryonic stages. Zoological Research, 2014, 35(2): 99-107.  doi: 10.11813/j.issn.0254-5853.2014.2.099
    [4] Hong-Mei ZHANG, Yan-Fang SU, Zhi-Yi SHI, Yuan-Shuai FU. cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis. Zoological Research, 2014, 35(4): 307-312.  doi: 10.13918/j.issn.2095-8137.2014.4.307
    [5] Wen-Qing ZHANG, Yi-Fu GONG, Li ZHANG, Jun LI, Xiao-Dan LIU. Cloning and expression analysis of the NFκB inhibitor IκBα of ayu (Plecoglossus altivelis). Zoological Research, 2013, 34(4): 399-405.  doi: 10.11813/j.issn.0254-5853.2013.4.0399
    [6] WANG Fang, CHEN Jiong, SHI Yu-Hong, LU Xin-Jiang, LI Ming-Yun. Molecular cloning, sequence analysis and stress-related changes of the heat shock protein 60 gene in Neobenedenia melleni. Zoological Research, 2012, 33(6): 603-608.  doi: 10.3724/SP.J.1141.2012.06603
    [7] CHAO Yan, ZHAO Lan-Ying, LI Chang-Zhong, XIE Bao-Sheng, SHEN Zhi-Xin, WANG Guo-Jie, WANG Zhen-Gang, LI Chao, BAI Bin-Qiang, ZHANG Hui, QI De-Lin. cDNA cloning and expression analysis of MSTN gene from Schizopygopsis pylzovi. Zoological Research, 2012, 33(5): 473-480.  doi: 10.3724/SP.J.1141.2012.05473
    [8] ZHANG Hao, FAN Chun-Xin, SONG Jia-Kun. Cloning and expression of Tbx3 gene in Siberian sturgeon, Acipenser baerii. Zoological Research, 2012, (2): 158-164.  doi: 10.3724/SP.J.1141.2012.02158
    [9] ZHUANG Yong-Hui, LI Si-Man, YU Guo-Yu, ZHANG Yong, XIANG Yang, ZOU Hao, LEE Wen-Hui. Bacterial expression and purification of biologically active human TFF2. Zoological Research, 2012, (2): 144-150.  doi: 10.3724/SP.J.1141.2012.02144
    [10] Jinling CAO, Jianjie CHEN, Zhongliang JIANG, Yongju LUO, Xi GAN. Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus. Zoological Research, 2012, 33(E3-4): 40-46.  doi: 10.3724/SP.J.1141.2012.E03-04E40
    [11] yu guo-Yu, XIANG Yang, ZHANG Hong-Yun, JIANG Ping, Lee Wen-Hui, ZHANG Yun, ZHANG. Expression of Bm-TFF2 mutants in Escherichia coli andtheir cell migration-promoting activity. Zoological Research, 2011, 32(4): 379-385.  doi: 10.3724/SP.J.1141.2011.04379
    [12] CHEN Wen-Bo, WANG Xin, ZHOU Ya-Lu, DONG Hai-Yan, LIN Hao-Ran, LI Wen-Sheng. Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus). Zoological Research, 2011, 32(3): 285-292.  doi: 10.3724/SP.J.1141.2011.03285
    [13] Yu guo-yu, ZHANG Hong-Yun, ZHANG Yong, JIANG Ping, Lee Wen-Hui, Zhang Yun. Expression and identification of Bm-TFF2 in Pichia pastoris. Zoological Research, 2010, 31(6): 565-569.  doi: 10.3724/SP.J.1141.2010.06565
    [14] Chen Xin-xin, Yu guo-yu, ZHAN Yan, Zhang Yun, Shen Ji-hong, Lee We. Antibacterial Activity Investigation of Human Semenogelin I Derived Peptides. Zoological Research, 2009, 30(2): 178-184.  doi: 10.3724/SP.J.1141.2009.02178
    [15] DENG Si-ping, CHEN Song-lin, LIU Ben-wei, XU Jian-yong, TIAN Yong-sheng. Molecular Cloning, Characterization and Expression Analysis of Brain P450arom in Half-smooth Tongue-sole, Cynoglossus semilaevis Gunther. Zoological Research, 2008, 29(1): 17-24.
    [16] DENG Si-ping, CHEN Song-lin, LIU Ben-wei. Molecular Cloning and Expression Analysis of FTZ-F1 in the Half-smooth Tongue-sole, Cynoglossus semilaevis. Zoological Research, 2008, 29(6): 592-598.  doi: 10.3724/SP.J.1141.2008.06592
    [17] PENG Wu, TANG Ya-nan, HU Cheng-yu. Characterization of CaPKR-like Expression in Tissues from Crucian and Grass Carp. Zoological Research, 2007, 28(5): 465-469.
    [18] CHENG Guo-feng, LIN Jiao-jiao, SUN Jun, LI Hao, ZHU Chuan-gang, ZHOU Yuan-cong, CAI You-min. Cloning and Functional Analysis of the Full Length cDNA Sequence of Eukaryotic Translation Initiation Factor 5 in Schistosoma japonicum. Zoological Research, 2005, 26(5): 513-517.
    [19] LIU Xu-sheng, LIANG Bing, ZHANG Shu-yi. cDNA Cloning,Tissue Distribution and mRNA Transcription of DMRT1 Gene in the Protandrous Black Porgy Acanthopagrus schlegeli. Zoological Research, 2004, 25(2): 158-161.
    [20] LI Chang-jian, LIU Jun, SHI Yao-hua, YANG Shu-ting, GUI Jian-fang. Full Length cDNA Cloning and Tissue Expression Specificity of Ran Gene in Color Crucian Carp. Zoological Research, 2003, 24(3): 173-179.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (3185) PDF downloads(4188) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return