Volume 29 Issue 2
Mar.  2008
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DU Chen-guang, YANG Yan-yan, LI Hai-jun, BAO Qing-jiang. Expression of the Ghrelin in Sheep Ooctyes and Pre-implantation Embryos Produced in vivo. Zoological Research, 2008, 29(2): 134-138. doi: 10.3724/SP.J.1141.2008.02134
Citation: DU Chen-guang, YANG Yan-yan, LI Hai-jun, BAO Qing-jiang. Expression of the Ghrelin in Sheep Ooctyes and Pre-implantation Embryos Produced in vivo. Zoological Research, 2008, 29(2): 134-138. doi: 10.3724/SP.J.1141.2008.02134

Expression of the Ghrelin in Sheep Ooctyes and Pre-implantation Embryos Produced in vivo

doi: 10.3724/SP.J.1141.2008.02134
  • Received Date: 2008-01-09
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2008-04-22
  • The possibility of ghrelin expressed in sheep oocytes and pre-implantation embryos produced in vivo was investigated in this study. The observed ghrelin immunoreactivity of oocytes and embryos at all stages was predominantly in the cytoplasm. Relative real-time reverse-transciptase (RT)-PCR experiments confirmed that the ghrelin mRNA levels varied depending on the developmental stage, with the highest expression in the blastocyst, metaphase II (MII) oocytes, 2- and 8-cell stages had a significantly higher expression in the germinal vesicle (GV) oocytes, 4-stage and morula. Dynamic changes and the persistent presence of the ghrelin signaling system within oocytes and pre-implantation embryos opens up the possibility of a potential regulatory role of this novel molecule during oocyte maturation and embryonic development.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Expression of the Ghrelin in Sheep Ooctyes and Pre-implantation Embryos Produced in vivo

doi: 10.3724/SP.J.1141.2008.02134

Abstract: The possibility of ghrelin expressed in sheep oocytes and pre-implantation embryos produced in vivo was investigated in this study. The observed ghrelin immunoreactivity of oocytes and embryos at all stages was predominantly in the cytoplasm. Relative real-time reverse-transciptase (RT)-PCR experiments confirmed that the ghrelin mRNA levels varied depending on the developmental stage, with the highest expression in the blastocyst, metaphase II (MII) oocytes, 2- and 8-cell stages had a significantly higher expression in the germinal vesicle (GV) oocytes, 4-stage and morula. Dynamic changes and the persistent presence of the ghrelin signaling system within oocytes and pre-implantation embryos opens up the possibility of a potential regulatory role of this novel molecule during oocyte maturation and embryonic development.

DU Chen-guang, YANG Yan-yan, LI Hai-jun, BAO Qing-jiang. Expression of the Ghrelin in Sheep Ooctyes and Pre-implantation Embryos Produced in vivo. Zoological Research, 2008, 29(2): 134-138. doi: 10.3724/SP.J.1141.2008.02134
Citation: DU Chen-guang, YANG Yan-yan, LI Hai-jun, BAO Qing-jiang. Expression of the Ghrelin in Sheep Ooctyes and Pre-implantation Embryos Produced in vivo. Zoological Research, 2008, 29(2): 134-138. doi: 10.3724/SP.J.1141.2008.02134

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