Expression of the Ghrelin in Sheep Ooctyes and Pre－implantation Embryos Produced in vivo
- Received Date: 2008-01-09
- Rev Recd Date: 1900-01-01
- Publish Date: 2008-04-22
Abstract: The possibility of ghrelin expressed in sheep oocytes and pre-implantation embryos produced in vivo was investigated in this study. The observed ghrelin immunoreactivity of oocytes and embryos at all stages was predominantly in the cytoplasm. Relative real-time reverse-transciptase (RT)-PCR experiments confirmed that the ghrelin mRNA levels varied depending on the developmental stage, with the highest expression in the blastocyst, metaphase II (MII) oocytes, 2- and 8-cell stages had a significantly higher expression in the germinal vesicle (GV) oocytes, 4-stage and morula. Dynamic changes and the persistent presence of the ghrelin signaling system within oocytes and pre-implantation embryos opens up the possibility of a potential regulatory role of this novel molecule during oocyte maturation and embryonic development.
|Citation:||DU Chen-guang, YANG Yan-yan, LI Hai-jun, BAO Qing-jiang. Expression of the Ghrelin in Sheep Ooctyes and Pre－implantation Embryos Produced in vivo. Zoological Research, 2008, 29(2): 134-138. doi: 10.3724/SP.J.1141.2008.02134|