Volume 31 Issue 1
Jan.  2010
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ZHANG Min, ZHAO Jin-Liang*, DENG Yan-Fei. Cloning and Tissue Expression Analysis of Creatine Kinase (M-CK) cDNA from the Mandarin Fish,Siniperca chuatsi. Zoological Research, 2010, 31(1): 77-83. doi: 10.3724/SP.J.1141. 2010.01077
Citation: ZHANG Min, ZHAO Jin-Liang*, DENG Yan-Fei. Cloning and Tissue Expression Analysis of Creatine Kinase (M-CK) cDNA from the Mandarin Fish,Siniperca chuatsi. Zoological Research, 2010, 31(1): 77-83. doi: 10.3724/SP.J.1141. 2010.01077

Cloning and Tissue Expression Analysis of Creatine Kinase (M-CK) cDNA from the Mandarin Fish,Siniperca chuatsi

doi: 10.3724/SP.J.1141. 2010.01077
  • Received Date: 2009-05-18
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2010-02-22
  • The creatine kinase(CK) cDNA from the mandarin fish Siniperca chuatsi was cloned by RT-PCR and rapid amplification of cDNA ends (RACE) methods. The structural characteristics and phylogeny of this gene were analyzed. Sequence analysis revealed a 1 586 bp cDNA sequence containing 92 bp 5′-untranslated region, 348 bp 3′-untranslated region and 1146 bp open reading frame (ORF), which encoded 381 amino acids. Conserved sequence blocks of vertebrate CKs and diagnostic boxes for the muscle CK(M-CK) isozyme were identified in S. chuatsi CK. Siniperca chuatsi CK showed a higher similarity with vertebrates M-CK isozyme than other CK isozymes (Brain CK, Mitochondrial CKs) and grouped with M-CK isozyme in CK phylogeny, which strongly supported that S. chuatsi CK belongs to M-CK isozyme type. Semi-quantitative RT-PCR analysis demonstrated that the M-CK transcript expression varied among the different tissues and was detected at a high level in skin, ovary, kidney, stomach, muscle and heart, but lower in eye, brain and liver.
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Cloning and Tissue Expression Analysis of Creatine Kinase (M-CK) cDNA from the Mandarin Fish,Siniperca chuatsi

doi: 10.3724/SP.J.1141. 2010.01077

Abstract: The creatine kinase(CK) cDNA from the mandarin fish Siniperca chuatsi was cloned by RT-PCR and rapid amplification of cDNA ends (RACE) methods. The structural characteristics and phylogeny of this gene were analyzed. Sequence analysis revealed a 1 586 bp cDNA sequence containing 92 bp 5′-untranslated region, 348 bp 3′-untranslated region and 1146 bp open reading frame (ORF), which encoded 381 amino acids. Conserved sequence blocks of vertebrate CKs and diagnostic boxes for the muscle CK(M-CK) isozyme were identified in S. chuatsi CK. Siniperca chuatsi CK showed a higher similarity with vertebrates M-CK isozyme than other CK isozymes (Brain CK, Mitochondrial CKs) and grouped with M-CK isozyme in CK phylogeny, which strongly supported that S. chuatsi CK belongs to M-CK isozyme type. Semi-quantitative RT-PCR analysis demonstrated that the M-CK transcript expression varied among the different tissues and was detected at a high level in skin, ovary, kidney, stomach, muscle and heart, but lower in eye, brain and liver.

ZHANG Min, ZHAO Jin-Liang*, DENG Yan-Fei. Cloning and Tissue Expression Analysis of Creatine Kinase (M-CK) cDNA from the Mandarin Fish,Siniperca chuatsi. Zoological Research, 2010, 31(1): 77-83. doi: 10.3724/SP.J.1141. 2010.01077
Citation: ZHANG Min, ZHAO Jin-Liang*, DENG Yan-Fei. Cloning and Tissue Expression Analysis of Creatine Kinase (M-CK) cDNA from the Mandarin Fish,Siniperca chuatsi. Zoological Research, 2010, 31(1): 77-83. doi: 10.3724/SP.J.1141. 2010.01077

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