High-continuity genome assembly of the jellyfish <i>Chrysaora quinquecirrha</i>

Wang-Xiao Xia; Hao-Rong Li; Jing-Hao Ge; Yao-Wu Liu; Hong-Hui Li; Yan-Hua Su; Hai-Zhen Wang; Hui-Fang Guo; Yu-Xuan Dai; Yao-Wen Liu; Xing-Chun Gou

doi:10.24272/j.issn.2095-8137.2020.258

Volume 42 Issue 1

Jan. 2021

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Article Navigation > Zoological Research > 2021 > 42(1): 130-134

Wang-Xiao Xia, Hao-Rong Li, Jing-Hao Ge, Yao-Wu Liu, Hong-Hui Li, Yan-Hua Su, Hai-Zhen Wang, Hui-Fang Guo, Yu-Xuan Dai, Yao-Wen Liu, Xing-Chun Gou. High-continuity genome assembly of the jellyfish Chrysaora quinquecirrha. Zoological Research, 2021, 42(1): 130-134. doi: 10.24272/j.issn.2095-8137.2020.258

Citation:

Wang-Xiao Xia, Hao-Rong Li, Jing-Hao Ge, Yao-Wu Liu, Hong-Hui Li, Yan-Hua Su, Hai-Zhen Wang, Hui-Fang Guo, Yu-Xuan Dai, Yao-Wen Liu, Xing-Chun Gou. High-continuity genome assembly of the jellyfish Chrysaora quinquecirrha. Zoological Research, 2021, 42(1): 130-134. doi: 10.24272/j.issn.2095-8137.2020.258

Citation:

Wang-Xiao Xia, Hao-Rong Li, Jing-Hao Ge, Yao-Wu Liu, Hong-Hui Li, Yan-Hua Su, Hai-Zhen Wang, Hui-Fang Guo, Yu-Xuan Dai, Yao-Wen Liu, Xing-Chun Gou. High-continuity genome assembly of the jellyfish Chrysaora quinquecirrha. Zoological Research, 2021, 42(1): 130-134. doi: 10.24272/j.issn.2095-8137.2020.258

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High-continuity genome assembly of the jellyfish Chrysaora quinquecirrha

doi: 10.24272/j.issn.2095-8137.2020.258

1.
Shaanxi Key Laboratory of Brain Disorders, Institute of Basic Translational Medicine, Xi’an Medical University, Xi’an, Shaanxi 710021, China
2.
Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan 650201, China
3.
Center for Ecological and Environmental Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China
4.
ZhiQiao Research Institute, Changsha, Hunan 410000, China
5.
Shaanxi Key Laboratory of Infection and Immune Disorders, School of Basic Medical Science, Xi’an Medical University, Xi’an, Shaanxi 710021, China

#Authors contributed equally to this work

Funds: This work was supported by the Province of China (202011840014) Shaanxi College Students’ Innovation and Entrepreneurship Training Program (S202011840014), Xi’an Medical University College Students’ Innovation and Entrepreneurship Training Program (121520014), National Natural Science Foundation of China (31760671), Joint Special Project of Agricultural Basic Research in Yunnan Province (2018FG001-041), Yunnan Provincial Department of Education Research Fund (2020J0251), Scientific Research Fund of Shaanxi Provincial Education Department (20JS143), and Natural Science Basic Research Plan in Shaanxi Province of China (2020JQ-876)

More Information

Corresponding author: E-mail: yaowenliu@foxmail.com; gouxingchun@189.cn
Received Date: 2020-09-07
Accepted Date: 2020-12-24

Available Online: 2020-12-28

Publish Date: 2021-01-18

Abstract

Abstract

The Atlantic sea nettle (Chrysaora quinquecirrha) has an important evolutionary position due to its high ecological value. However, due to limited sequencing technologies and complex jellyfish genomic sequences, the current C. quinquecirrha genome assembly is highly fragmented. Here, we used the most advanced high-throughput chromosome conformation capture (Hi-C) technology to obtain high-coverage sequencing data of the C. quinquecirrha genome. We then anchored these data to the previously published contig-level assembly to improve the genome. Finally, a high-continuity genome sequence of C. quinquecirrha was successfully assembled, which contained 1 882 scaffolds with a N50 length of 3.83 Mb. The N50 length of the genome assembly was 5.23 times longer than the previously released one, and additional analysis revealed that it had a high degree of genomic continuity and accuracy. Acquisition of the high-continuity genome sequence of C. quinquecirrha not only provides a basis for the study of jellyfish evolution through comparative genomics but also provides an important resource for studies on jellyfish growth and development.
- Jellyfish;,
- Genome,
- Assembly,
- Hi-C,
- High continuity,
- Evolution

#Authors contributed equally to this work

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References(25)

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