Volume 41 Issue 5
Sep.  2020
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Yu-Ping Cao, Qing-Qing Lin, Wan-Yun He, Jing Wang, Meng-Ying Yi, Lu-Chao Lv, Jun Yang, Jian-Hua Liu, Jian-Ying Guo. Co-selection may explain the unexpectedly high prevalence of plasmid-mediated colistin resistance gene mcr-1 in a Chinese broiler farm. Zoological Research, 2020, 41(5): 569-575. doi: 10.24272/j.issn.2095-8137.2020.131
Citation: Yu-Ping Cao, Qing-Qing Lin, Wan-Yun He, Jing Wang, Meng-Ying Yi, Lu-Chao Lv, Jun Yang, Jian-Hua Liu, Jian-Ying Guo. Co-selection may explain the unexpectedly high prevalence of plasmid-mediated colistin resistance gene mcr-1 in a Chinese broiler farm. Zoological Research, 2020, 41(5): 569-575. doi: 10.24272/j.issn.2095-8137.2020.131

Co-selection may explain the unexpectedly high prevalence of plasmid-mediated colistin resistance gene mcr-1 in a Chinese broiler farm

doi: 10.24272/j.issn.2095-8137.2020.131
Funds:  This work was supported in part by the National Natural Science Foundation of China (31830099, 31902322), International Science and Technology Cooperation Project of Xinjiang Production and Construction Corps(XPCC) (2019BC004), Guangdong Special Support Program Innovation Team (2019BT02N054), and Innovation Team Project of Guangdong University (2019KCXTD001)
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  • Corresponding author: E-mail: jhliu@scau.edu.cnjyguo@scau.edu.cn
  • Received Date: 2020-05-30
  • Accepted Date: 2020-07-21
  • Available Online: 2020-07-31
  • Publish Date: 2020-09-20
  • The rise of the plasmid-encoded colistin resistance gene mcr-1 is a major concern globally. Here, during a routine surveillance, an unexpectedly high prevalence of Escherichia coli with reduced susceptibility to colistin (69.9%) was observed in a Chinese broiler farm. Fifty-three (63.9%) E. coli isolates were positive for mcr-1. All identified mcr-1-positive E. coli (MCREC) were multidrug resistant and carried other clinically significant resistance genes. Furthermore, the mcr-1 genes were mainly located on the IncI2 and IncHI2 plasmids. Conjugation experiments unraveled the co-transfer of mcr-1 with other antibiotic resistance genes (blaCTX-M-55, blaCTX-M-14, floR, and fosA3) via the IncI2 (n=3) and IncHI2 (n=4) plasmids. The stable genetic context mcr-1-pap2 was common in the IncI2 plasmids, whereas ISApl1-mcr-1-pap2-ISApl1 was mainly found in the IncHI2 plasmids. The dominance of mcr-1-bearing IncI2 and IncHI2 plasmids and co-selection of mcr-1 with other antimicrobial resistance genes might contribute to the exceptionally high prevalence of mcr-1 in this broiler farm. Our results emphasized the importance of appropriate antibiotic use in animal production.
  • #Authors contributed equally to this work
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