Volume 41 Issue 3
May  2020
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Article Contents
Yang Yang, Li-Na Wu, Jing-Fang Chen, Xi Wu, Jun-Hong Xia, Zi-Ning Meng, Xiao-Chun Liu, Hao-Ran Lin. Whole-genome sequencing of leopard coral grouper (Plectropomus leopardus) and exploration of regulation mechanism of skin color and adaptive evolution. Zoological Research, 2020, 41(3): 328-340. doi: 10.24272/j.issn.2095-8137.2020.038
Citation: Yang Yang, Li-Na Wu, Jing-Fang Chen, Xi Wu, Jun-Hong Xia, Zi-Ning Meng, Xiao-Chun Liu, Hao-Ran Lin. Whole-genome sequencing of leopard coral grouper (Plectropomus leopardus) and exploration of regulation mechanism of skin color and adaptive evolution. Zoological Research, 2020, 41(3): 328-340. doi: 10.24272/j.issn.2095-8137.2020.038

Whole-genome sequencing of leopard coral grouper (Plectropomus leopardus) and exploration of regulation mechanism of skin color and adaptive evolution

doi: 10.24272/j.issn.2095-8137.2020.038
Funds:  This study was supported by the Agriculture Research System of China (ARS-47); Science and Technology Planning Project of Guangzhou (201804020013); National Natural Science Foundation of China (31872572; u1401213; 31802266); Yang Fan Innovative & Entrepreneurial Research Team Project (No.201312H10); and Program of the China-ASEAN Maritime Cooperation Fund of the Chinese Government (42000-41170002)
More Information
  • Leopard coral groupers belong to the Plectropomus genus of the Epinephelidae family and are important fish for coral reef ecosystems and the marine aquaculture industry. To promote future research of this species, a high-quality chromosome-level genome was assembled using PacBio sequencing and Hi-C technology. A 787.06 Mb genome was assembled, with 99.7% (784.57 Mb) of bases anchored to 24 chromosomes. The leopard coral grouper genome size was smaller than that of other groupers, which may be related to its ancient status among grouper species. A total of 22 317 protein-coding genes were predicted. This high-quality genome of the leopard coral grouper is the first genomic resource for Plectropomus and should provide a pivotal genetic foundation for further research. Phylogenetic analysis of the leopard coral grouper and 12 other fish species showed that this fish is closely related to the brown-marbled grouper. Expanded genes in the leopard coral grouper genome were mainly associated with immune response and movement ability, which may be related to the adaptive evolution of this species to its habitat. In addition, we also identified differentially expressed genes (DEGs) associated with carotenoid metabolism between red and brown-colored leopard coral groupers. These genes may play roles in skin color decision by regulating carotenoid content in these groupers.

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