Volume 40 Issue 5
Sep.  2019
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Yi-Zhuo Sun, Si-Tong Liu, Xiao-Meng Li, Kang Zou. Progress in in vitro culture and gene editing of porcine spermatogonial stem cells. Zoological Research, 2019, 40(5): 343-348. doi: 10.24272/j.issn.2095-8137.2019.051
Citation: Yi-Zhuo Sun, Si-Tong Liu, Xiao-Meng Li, Kang Zou. Progress in in vitro culture and gene editing of porcine spermatogonial stem cells. Zoological Research, 2019, 40(5): 343-348. doi: 10.24272/j.issn.2095-8137.2019.051

Progress in in vitro culture and gene editing of porcine spermatogonial stem cells

doi: 10.24272/j.issn.2095-8137.2019.051
Funds:  the Fundamental Research Funds for the Central Universities in China(KYDS201807);China(2016YFE0128500)
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  • Corresponding author: Xiao-Meng Li,Kang Zou
  • Received Date: 2019-05-09
  • Publish Date: 2019-09-18
  • Research on in vitro culture and gene editing of domestic spermatogonial stem cells (SSCs) is of considerable interest but remains a challenging issue in animal science. In recent years, some progress on the isolation, purification, and genetic manipulation of porcine SSCs has been reported. Here, we summarize the characteristics of porcine SSCs as well current advances in their in vitro culture, potential usage, and genetic manipulation. Furthermore, we discuss the current application of gene editing in pig cloning technology. Collectively, this commentary aims to summarize the progress made and obstacles encountered in porcine SSC research to better serve animal husbandry, improve livestock fecundity, and enhance potential clinical use.
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Progress in in vitro culture and gene editing of porcine spermatogonial stem cells

doi: 10.24272/j.issn.2095-8137.2019.051
Funds:  the Fundamental Research Funds for the Central Universities in China(KYDS201807);China(2016YFE0128500)
    Corresponding author: Xiao-Meng Li,Kang Zou

Abstract: Research on in vitro culture and gene editing of domestic spermatogonial stem cells (SSCs) is of considerable interest but remains a challenging issue in animal science. In recent years, some progress on the isolation, purification, and genetic manipulation of porcine SSCs has been reported. Here, we summarize the characteristics of porcine SSCs as well current advances in their in vitro culture, potential usage, and genetic manipulation. Furthermore, we discuss the current application of gene editing in pig cloning technology. Collectively, this commentary aims to summarize the progress made and obstacles encountered in porcine SSC research to better serve animal husbandry, improve livestock fecundity, and enhance potential clinical use.

Yi-Zhuo Sun, Si-Tong Liu, Xiao-Meng Li, Kang Zou. Progress in in vitro culture and gene editing of porcine spermatogonial stem cells. Zoological Research, 2019, 40(5): 343-348. doi: 10.24272/j.issn.2095-8137.2019.051
Citation: Yi-Zhuo Sun, Si-Tong Liu, Xiao-Meng Li, Kang Zou. Progress in in vitro culture and gene editing of porcine spermatogonial stem cells. Zoological Research, 2019, 40(5): 343-348. doi: 10.24272/j.issn.2095-8137.2019.051
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