Volume 40 Issue 4
Jul.  2019
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Jian-Peng Chen, Wei Pang, Zi-Wei Zhao, Yan-Hui Bi, Xiao-Wu Chen. Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway. Zoological Research, 2019, 40(4): 337-342. doi: 10.24272/j.issn.2095-8137.2019.028
Citation: Jian-Peng Chen, Wei Pang, Zi-Wei Zhao, Yan-Hui Bi, Xiao-Wu Chen. Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway. Zoological Research, 2019, 40(4): 337-342. doi: 10.24272/j.issn.2095-8137.2019.028

Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway

doi: 10.24272/j.issn.2095-8137.2019.028
Funds:  This study was supported by a grant from the Key Project of Scientific and Technological Innovation of Blue Granary (2018YFD0901505)
More Information
  • Corresponding author: Xiao-Wu Chen
  • Received Date: 2018-12-11
  • Publish Date: 2019-07-18
  • Hemorrhagic septicemia is an acute, highly fatal disease that affects goldfish (Carassius auratus). To gain a better understanding of related immune genes, the transcriptomes of the skin and head kidney of goldfish suffering hemorrhagic septicemia were sequenced, assembled, and characterized. Based on functional annotation, an extensive and diverse catalog of expressed genes were identified in both the skin and head kidney. As two different organs, pair-wise comparison identified 122/77 unigenes up/down-regulated (two-fold change with P<0.05) in the skin and head kidney. Most genes of the immune pathways were expressed and isolated in both skin and head kidney, including interferon (IFN) transcription factors 1–10 and Toll-like receptors (TLRs). Interferon regulatory factor 3 (IRF3), a key IFN transcription factor, was up-regulated at the transcriptional level by polyriboinosinic: polyribocytidylic acid (poly I:C) challenge and regulated the IFN response by increasing the activity of IFN-β and IFN-stimulated response element (ISRE)-containing promoter. This study will benefit the identification and understanding of novel genes that play important roles in the immunological reactions of fish suffering from hemorrhagic septicemia.
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Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway

doi: 10.24272/j.issn.2095-8137.2019.028
Funds:  This study was supported by a grant from the Key Project of Scientific and Technological Innovation of Blue Granary (2018YFD0901505)
    Corresponding author: Xiao-Wu Chen

Abstract: Hemorrhagic septicemia is an acute, highly fatal disease that affects goldfish (Carassius auratus). To gain a better understanding of related immune genes, the transcriptomes of the skin and head kidney of goldfish suffering hemorrhagic septicemia were sequenced, assembled, and characterized. Based on functional annotation, an extensive and diverse catalog of expressed genes were identified in both the skin and head kidney. As two different organs, pair-wise comparison identified 122/77 unigenes up/down-regulated (two-fold change with P<0.05) in the skin and head kidney. Most genes of the immune pathways were expressed and isolated in both skin and head kidney, including interferon (IFN) transcription factors 1–10 and Toll-like receptors (TLRs). Interferon regulatory factor 3 (IRF3), a key IFN transcription factor, was up-regulated at the transcriptional level by polyriboinosinic: polyribocytidylic acid (poly I:C) challenge and regulated the IFN response by increasing the activity of IFN-β and IFN-stimulated response element (ISRE)-containing promoter. This study will benefit the identification and understanding of novel genes that play important roles in the immunological reactions of fish suffering from hemorrhagic septicemia.

Jian-Peng Chen, Wei Pang, Zi-Wei Zhao, Yan-Hui Bi, Xiao-Wu Chen. Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway. Zoological Research, 2019, 40(4): 337-342. doi: 10.24272/j.issn.2095-8137.2019.028
Citation: Jian-Peng Chen, Wei Pang, Zi-Wei Zhao, Yan-Hui Bi, Xiao-Wu Chen. Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway. Zoological Research, 2019, 40(4): 337-342. doi: 10.24272/j.issn.2095-8137.2019.028
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