Volume 40 Issue 4
Jul.  2019
Turn off MathJax
Article Contents
Jian-Peng Chen, Wei Pang, Zi-Wei Zhao, Yan-Hui Bi, Xiao-Wu Chen. Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway. Zoological Research, 2019, 40(4): 337-342. doi: 10.24272/j.issn.2095-8137.2019.028
Citation: Jian-Peng Chen, Wei Pang, Zi-Wei Zhao, Yan-Hui Bi, Xiao-Wu Chen. Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway. Zoological Research, 2019, 40(4): 337-342. doi: 10.24272/j.issn.2095-8137.2019.028

Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway

doi: 10.24272/j.issn.2095-8137.2019.028
Funds:  This study was supported by a grant from the Key Project of Scientific and Technological Innovation of Blue Granary (2018YFD0901505)
More Information
  • Corresponding author: Xiao-Wu Chen
  • Received Date: 2018-12-11
  • Publish Date: 2019-07-18
  • Hemorrhagic septicemia is an acute, highly fatal disease that affects goldfish (Carassius auratus). To gain a better understanding of related immune genes, the transcriptomes of the skin and head kidney of goldfish suffering hemorrhagic septicemia were sequenced, assembled, and characterized. Based on functional annotation, an extensive and diverse catalog of expressed genes were identified in both the skin and head kidney. As two different organs, pair-wise comparison identified 122/77 unigenes up/down-regulated (two-fold change with P<0.05) in the skin and head kidney. Most genes of the immune pathways were expressed and isolated in both skin and head kidney, including interferon (IFN) transcription factors 1–10 and Toll-like receptors (TLRs). Interferon regulatory factor 3 (IRF3), a key IFN transcription factor, was up-regulated at the transcriptional level by polyriboinosinic: polyribocytidylic acid (poly I:C) challenge and regulated the IFN response by increasing the activity of IFN-β and IFN-stimulated response element (ISRE)-containing promoter. This study will benefit the identification and understanding of novel genes that play important roles in the immunological reactions of fish suffering from hemorrhagic septicemia.
  • loading
  • [1]
    null Akira S , Takeda K . 2004. Toll-like receptor signalling. Nature Reviews Immunology, 4(7): 499–511.
    null Alvarez-Pellitero P . 2008. Fish immunity and parasite infections: from innate immunity to immunoprophylactic prospects. Veterinary Immunology and Immunopathology, 126(3–4): 171–198.
    null Avunje S , Kim WS , Park CS , Oh MJ , Jung SJ . 2011. Toll-like receptors and interferon associated immune factors in viral haemorrhagic septicaemia virus-infected olive flounder (Paralichthys olivaceus). Fish & Shellfish Immunology, 31(3): 407–414.
    null Beck BH , Peatman E . 2015. Mucosal Health in Aquaculture. Amsterdam, New York: Elsevier/AP, Academic Press.
    null Bos JD , Kapsenberg ML . 1993. The skin immune system: progress in cutaneous biology. Immunology Today, 14(2): 75–78.
    null Chen J , Li C , Huang R , Du F , Liao L , Zhu Z , Wang Y . 2012. Transcriptome analysis of head kidney in grass carp and discovery of immune-related genes. BMC Beterinary Research, 8: 108.
    null Choe Y , Yu JE , Park J , Park D , Oh JI, Kim S , Moon KH , Kang HY . 2017. Goldfish, Carassius auratus, as an infection model for studying the pathogenesis of Edwardsiella piscicida . Veterinary Research Communications, 41(4): 289–297.
    null Gerdol M , Buonocore F , Scapigliati G , Pallavicini A . 2015. Analysis and characterization of the head kidney transcriptome from the Antarctic fish Trematomus bernacchii (Teleostea, Notothenioidea): a source for immune relevant genes. Marine Genomics, 20: 13–15.
    null Gu YF , Wei Q , Tang SJ , Chen XW , Zhao JL . 2016. Molecular characterization and functional analysis of IRF3 in tilapia (Oreochromis niloticus). Developmental and Comparative Immunology, 55: 130–137.
    null Hatten F , Fredriksen A , Hordvik I , Endresen C . 2001. Presence of IgM in cutaneous mucus, but not in gut mucus of Atlantic salmon, Salmo salar . Serum IgM is rapidly degraded when added to gut mucus. Fish & Shellfish Immunology, 11(3): 257–268.
    null Holland JW , Bird S , Williamson B , Woudstra C , Mustafa A , Wang T , Zou J , Blaney SC , Collet B , Secombes CJ . 2008. Molecular characterization of IRF3 and IRF7 in rainbow trout, Oncorhynchus mykiss: functional analysis and transcriptional modulation. Molecular Immunology, 46(2): 269–285.
    null Honda K , Taniguchi T . 2006. IRFs: master regulators of signalling by Toll-like receptors and cytosolic pattern-recognition receptors. Nature Reviews Immunology, 6(9): 644–658.
    null Itaya H , Oshita K , Arakawa K , Tomita M . 2013. GEMBASSY: an EMBOSS associated software package for comprehensive genome analyses. Source Code for Biology and Medicine, 8(1): 17.
    null Kobayashi I , Sekiya M , Moritomo T , Ototake M , Nakanishi T . 2006. Demonstration of hematopoietic stem cells in ginbuna carp (Carassius auratus langsdorfii) kidney. Developmental and Comparative Immunology, 30(11): 1034–1046.
    null Micallef G , Bickerdike R , Reiff C , Fernandes JM , Bowman AS , Martin SA . 2012. Exploring the transcriptome of Atlantic salmon (Salmo salar) skin, a major defense organ. Marine Biotechnology (New York, N.Y.), 14(5): 559–569.
    null Montero J , Chaves-Pozo E , Cuesta A , Tafalla C . 2009. Chemokine transcription in rainbow trout (Oncorhynchus mykiss) is differently modulated in response to viral hemorrhagic septicaemia virus (VHSV) or infectious pancreatic necrosis virus (IPNV). Fish & Shellfish Immunology, 27(6): 661–669.
    null Morimoto T , Biswas G , Kono T , Sakai M , Hikima J . 2016. Immune responses in the Japanese pufferfish (Takifugu rubripes) head kidney cells stimulated with particulate silica. Fish & Shellfish Immunology, 49: 84–90.
    null Mortazavi A , Williams BA, Mccue K , Schaeffer L , Wold B . 2008. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nature Methods, 5(7): 621–628.
    null Nuutila K , Siltanen A , Peura M , Bizik J , Kaartinen I , Kuokkanen H , Nieminen T , Harjula A , Aarnio P , Vuola J , Kankuri E . 2012. Human skin transcriptome during superficial cutaneous wound healing. Wound Repair and Regeneration, 20(6): 830–839.
    null Palaksha KJ , Shin GW , Kim YR , Jung TS . 2008. Evaluation of non-specific immune components from the skin mucus of olive flounder (Paralichthys olivaceus). Fish & Shellfish Immunology, 24(4): 479–488.
    null Paun A , Pitha PM . 2007. The IRF family, revisited. Biochimie, 89(6–7): 744–753.
    null Press CM , ? Evensen . 1999. The morphology of the immune system in teleost fishes. Fish & Shellfish Immunology, 9(4): 309–318.
    null Shin HS , Choi CY . 2014. The stimulatory effect of LED light spectra on genes related to photoreceptors and skin pigmentation in goldfish (Carassius auratus). Fish Physiology and Biochemistry, 40(4): 1229–1238.
    null Sun F , Zhang YB , Liu TK , Gan L , Yu FF , Liu Y , Gui JF . 2010. Characterization of fish IRF3 as an IFN-inducible protein reveals evolving regulation of IFN response in vertebrates. The Journal of Immunology, 185(12): 7573–7582.
    null Taniguchi T , Ogasawara K , Takaoka A , Tanaka N . 2001. IRF family of transcription factors as regulators of host defense. Annual Review of Immunology, 19: 623–655.
    null Vasta GR , Nita-Lazar M , Giomarelli B , Ahmed H , Du S , Cammarata M , Parrinello N , Bianchet MA , Amzel LM . 2011. Structural and functional diversity of the lectin repertoire in teleost fish: relevance to innate and adaptive immunity. Developmental and Comparative immunology, 35(12): 1388–1399.
    null Wang L , Feng Z , Wang X , Wang X , Zhang X . 2010. DEGseq: an R package for identifying differentially expressed genes from RNA-seq data. Bioinformatics, 26(1): 136–138.
    null Wang CL , Wang ZP , Wang JQ , Li MY , Chen XW . 2016. Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder). Zoological Research, 37(5): 301–306.
  • Relative Articles

    [1] SHEN Wen-Ying, REN Gang, ZHU Yao-Rong. Effects of compensatory growth on the levels of IGF-1, IGFBP-1 and expressions of IGF-1 mRNA, IGF-1R mRNA in Carassius auratus gibelio. Zoological Research, 2012, 33(3): 298-303.  doi: 10.3724/SP.J.1141.2012.03298
    [2] MA Dong-mei, ZHU Hua-ping, GUI Jian-fang. Molecular Cloning and Expression Characterization of Prox1 in Goldfish (Carassius auratus) Eye Development. Zoological Research, 2007, 28(6): 589-596.
    [3] HU Cheng-yu, XIE Zong-bo, ZHANG Yi-bing, CHEN Yu-dong, DENG Zheng-dong et al.. Binding of the Zα Domain from a Carassius auratus Protein Kinase PKR-like to Polyinosinic:Polycytidylic Acid. Zoological Research, 2005, 26(3): 237-242.
    [4] CHEN Yun-gui, YE Ding, SONG Ping, LV Dau-yuan, GUI Jian-fang. Expression and Distributing of vasa Gene During Gametogenesis of Goldfish (Carassius auratus). Zoological Research, 2005, 26(2): 179-183.
    [5] YUE Xing-jian, ZHANG Yao-guang, AO Lei, WANG Zhi-jian. Histology and Ultrastructure of Head Kidney in the Southern Catfish,Silurus meridionalis Chen. Zoological Research, 2004, 25(4): 327-333.
    [6] CHEN Yao, WANG Shi-quan, HAN Xiao-dong, HOU Ya-yi. Purification and Partial Characterization of Immunoglobulin M from Carassius auratus Serum and Skin Mucus. Zoological Research, 2003, 24(2): 111-115.
    [7] SHEN Wen-ying, ZHANG Li-hong, ZHENG Yong-ping, ZHOU Liu-qin, ZHENG Jian-ping. Effects of Starvation on Blood Composition and Ovarian Development in Carassius auratus gibelio. Zoological Research, 2003, 24(6): 441-444.
    [8] ZHOU Li, LIU Jin-Xia, GUI Jian-Fang. Preliminary Investigation on Genetic Diversity of Gynogenetic Silver Crucian Carp (Carassius auratus gibelio Bloch) Detected by Microsatellite DNA. Zoological Research, 2001, 22(4): 257-264.
    [9] FLU Quan-zhang. The Composition of immune Cells in Head Kidney of Grass Carp (Ctenopharyngodon idellus) Together With Ontogenic Change of Their Numbers. Zoological Research, 1998, 19(1): 11-16.
    [10] LIANG Qian-jin, PENG Yi-xin. An Analysis of The Muscle Proteins of The Wild Crucian (Carassius auratus) and Five Representative Varieties of Goldfishes (C.auratus var.) By Electrophoresis Methods. Zoological Research, 1994, 15(2): 68-75.
    [11] WU Duan-sheng, LIU Jun. Cytology Study on The Cross Fertilization Between Carassius auratus Red Variety (♀) and Cyprinus carpio Xiangjianesis (♂). Zoological Research, 1993, 14(3): 277-282.
    [12] ZHANG Tian-yin, FENG Shu-mang, PAN Zhong-zong. Scanning Electron Microscopic Observations on The Sperm Entry Into The Eggs of Goldfish,Carassius auratus. Zoological Research, 1993, 14(2): 166-170.
    [13] MENG Qing-wen. Studies on The Blood Vessels of Eye,Gas Bladder and Kidney of Snakehead. Zoological Research, 1990, 11(4): 265-271.
    [14] WANG Rui-fang, SHI Li-ming, HE Wei-shun. The karyotype of Carassius auratus From ER hai Lake. Zoological Research, 1989, 10(2): 169-170.
    [15] WANG Rui-fang, SHI Li-ming, HE Wei-shun. A Comparative Study of the Ag-NORS of Carassius auratus from Different Geographic Districts. Zoological Research, 1988, 9(2): 165-169.
    [16] HUANG Guo-ping, YAN Shao-yi. Electron Microscopic Observation on Annulate Lamellae During Oogenesis in Goldfish (Carassius Auratus). Zoological Research, 1988, 9(3): 209-215.
    [17] RAN Yong-lu, Anthony T. Tu. Studies on Purification and Characterization of Hemorrhagic Toxins from Lachesis muta Venom. Zoological Research, 1987, 8(4): 365-373.
    [18] ZHAI Zi-yu, KO Hong-wen et al., GONG Ming-yao. Viral Hemorrhagic Disease of Black Carp (Mylopharyngodon piceus). Zoological Research, 1987, 8(4): 379-386.
    [19] ZHU Hong-wen, WANG Hao, QIN Guo-qing. Studies on the Blood Cell Morphology of Crucian Carp (Carassius auratus L.). Zoological Research, 1985, 6(2): 147-153.
    [20] CUI Yue-li, ZAN Rui-guang. Studies on Gynogenesis of the High-Backed Form of Carassius auratus in Kunming Lake. Zoological Research, 1982, 3(zk): 237-242.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (602) PDF downloads(245) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint