Volume 40 Issue 2
Mar.  2019
Turn off MathJax
Article Contents
Yi Ren, Shui-Fang Liu, Li Nie, Shi-Yu Cai, Jiong Chen. Involvement of ayu NOD2 in NF-κB and MAPK signaling pathways: Insights into functional conservation of NOD2 in antibacterial innate immunity. Zoological Research, 2019, 40(2): 77-88. doi: 10.24272/j.issn.2095-8137.2018.066
Citation: Yi Ren, Shui-Fang Liu, Li Nie, Shi-Yu Cai, Jiong Chen. Involvement of ayu NOD2 in NF-κB and MAPK signaling pathways: Insights into functional conservation of NOD2 in antibacterial innate immunity. Zoological Research, 2019, 40(2): 77-88. doi: 10.24272/j.issn.2095-8137.2018.066

Involvement of ayu NOD2 in NF-κB and MAPK signaling pathways: Insights into functional conservation of NOD2 in antibacterial innate immunity

doi: 10.24272/j.issn.2095-8137.2018.066
More Information
  • Corresponding author: Li Nie, Jiong Chen,
  • Publish Date: 2019-03-18
  • Nucleotide oligomerization domain 2 (NOD2) is a major cytoplasmic sensor for pathogens and is critical for the clearance of cytosolic bacteria in mammals. However, studies regarding NOD2, especially the initiated signaling pathways, are scarce in teleost species. In this study, we identified a NOD2 molecule (PaNOD2) from ayu (Plecoglossus altivelis). Bioinformatics analysis showed the structure of NOD2 to be highly conserved during vertebrate evolution. Dual-luciferase reporter assays examined the activation of NF-κB signaling and Western blotting analysis detected the phosphorylation of three MAP kinases (p-38, Erk1/2, and JNK1/2). Functional study revealed that, like its mammalian counterparts, PaNOD2 was the receptor of the bacterial cell wall component muramyl dipeptide (MDP), and the leucine-rich repeat motif was responsible for the recognition and binding of PaNOD2 with the ligand. Overexpression of PaNOD2 activated the NF-κB signaling pathway, leading to the upregulation of inflammatory cytokines, including TNF-α and IL-1β in HEK293T cells and ayu head kidney-derived monocytes/macrophages (MO/MΦ). Particularly, we found that PaNOD2 activated the MAPK signaling pathways, as indicated by the increased phosphorylation of p-38, Erk1/2, and JNK1/2, which have not been characterized in any teleost species previously. Our findings proved that the NOD2 molecule and initiated pathways are conserved between mammals and ayu. Therefore, ayu could be used as an animal model to investigate NOD2-based diseases and therapeutic applications.
  • loading
  • Relative Articles

    [1] Yan Liang, Heng Li, Jing Li, Ze-Ning Yang, Jia-Li Li, Hui-Wen Zheng, Yan-Li Chen, Hai-Jing Shi, Lei Guo, Long-Ding Liu. Role of neutrophil chemoattractant CXCL5 in SARS-CoV-2 infection-induced lung inflammatory innate immune response in an in vivo hACE2 transfection mouse model. Zoological Research, 2020, 41(6): 621-631.  doi: 10.24272/j.issn.2095-8137.2020.118
    [2] Jian-Peng Chen, Wei Pang, Zi-Wei Zhao, Yan-Hui Bi, Xiao-Wu Chen. Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway. Zoological Research, 2019, 40(4): 337-342.  doi: 10.24272/j.issn.2095-8137.2019.028
    [3] Wei-Na Guo, Bin Zhu, Ling Ai, Dong-Liang Yang, Bao-Ju Wang.  Animal models for the study of hepatitis B virus infection. Zoological Research, 2018, 39(1): 25-31.  doi: 10.24272/j.issn.2095-8137.2018.013
    [4] Lei Guo, Yan-Cui Wang, Jun-Jie Mei, Ruo-Tong Ning, Jing-Jing Wang, Jia-Qi Li, Xi Wang, Hui-Wen Zheng, Hai-Tao Fan, Long-Ding Liu. Pulmonary immune cells and inflammatory cytokine dysregulation are associated with mortality of IL-1R1-/-mice infected with influenza virus (H1N1). Zoological Research, 2017, 38(3): 146-154.  doi: 10.24272/j.issn.2095-8137.2017.035
    [5] Jing WANG, Guang LI, Guang-Hui QIAN, Jun-Hao HUA, Yi-Quan WANG. Expression analysis of eight amphioxus genes involved in the Wnt/β-catenin signaling pathway. Zoological Research, 2016, 37(3): 136-143.  doi: 10.13918/j.issn.2095-8137.2016.3.136
    [6] Jun WU, Yu-Hong SHI, Xue-Heng ZHANG, Chang-Hong LI, Ming-Yun LI, Jiong CHEN. Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection. Zoological Research, 2015, 36(3): 133-141.
    [7] Jin-Hua WANG, Liang MIAO, Ming-Yun LI, Xiao-Fei GUO, Na PAN, Ying-Ying CHEN, Liang ZHAO. Cloning the Dmrt1 and DmrtA2 genes of ayu (Plecoglossus altivelis) and mapping their expression in adult, larval, and embryonic stages. Zoological Research, 2014, 35(2): 99-107.  doi: 10.11813/j.issn.0254-5853.2014.2.099
    [8] Wen-Qing ZHANG, Yi-Fu GONG, Li ZHANG, Jun LI, Xiao-Dan LIU. Cloning and expression analysis of the NFκB inhibitor IκBα of ayu (Plecoglossus altivelis). Zoological Research, 2013, 34(4): 399-405.  doi: 10.11813/j.issn.0254-5853.2013.4.0399
    [9] YAN Mao-Cang, SHAN Le-Zhou, CHEN Shao-Bo, XIE Qi-Lang. Effects of three antigens extracted from Vibrio vulnificus on the immunological protection of Nibea albiflora. Zoological Research, 2012, 33(5): 503-509.  doi: 10.3724/SP.J.1141.2012.05503
    [10] SHI Yu-Hong, CHEN Jiong, GAO Shan-Shan, SHEN Guang-Qiang, LU Xin-Jiang, LI Ming-Yun. Cloning, physical and chemical property analysis of the Japanese sea bass Wap65-2 gene and its expression following Vibrio harveyi infection. Zoological Research, 2012, 33(5): 481-486.  doi: 10.3724/SP.J.1141.2012.05481
    [11] JIN Li-Fang, JI Shao-Hui, YANG Ji-Feng, JI Wei-Zhi. Notch signaling dependent differentiation of cholangiocyte-likecells from rhesus monkey embryonic stem cells. Zoological Research, 2011, 32(4): 391-395.  doi: 10.3724/SP.J.1141.2011.04391
    [12] HUANG Zuo-An, CHEN Jiong, LU Xin-Jiang, SHI Yu-Hong, LI Ming-Yun. Alteration on the expression of ayu coagulation factor X gene upon Listonella anguillarum infection. Zoological Research, 2011, 32(5): 492-498.  doi: 10.3724/SP.J.1141.2011.05492
    [13] WANG Jian-hua, WANG Yuan-yuan, OUYANG Dong-yun, ZHENG Yong-tang. Apoptosis in Human Immunodeficiency Virus Infection. Zoological Research, 2002, 23(6): 514-520.
    [14] XU Xia-Lian, ZHAO Xiao-Fan, WANG Jin-Xing. Distribution and Biosynthesis Sites of Cathepsin B in Helicoverpa armigera. Zoological Research, 2001, 22(3): 242-245.
    [15] ZHA Hong-Guang, ZHANG Yun. Purification of a Phospholipase A[2] from Bungarus fasciatus Venom by One Step Sepharose 4B Method. Zoological Research, 2001, 22(6): 433-436.
    [16] LI Yi-ping, SHEN Hong, GU Zheng, ZUO Jia-ke. A Preliminary Study on The Oviductal Cytokines in Controlling The Development of Mammalian Early Embryos. Zoological Research, 1997, 18(1): 85-92.
    [17] CONG Ying-zi, YU Shi-guang. Regulation of Surface IgD Expression on Murine B Cells. Zoological Research, 1994, 15(3): 81-86.
    [18] LING Fa-yao, SHI Li-ming. The Study of B Chromosomes In Drosophila albomicans 2.The Reiation Between B Chromosomes and The Vitality of Kunming Population. Zoological Research, 1992, 13(3): 271-275.
    [19] LING Fa-yao, Osamu Kitagawa. The Study of B Chromosomes In Drosophila albomicans Ⅰ. The Number and Frequency of Bs In Kunming Population. Zoological Research, 1991, 12(1): 93-98.
    [20] HE Quan-pin, LIN Si-jun, XU Yi-sheng, YAO Zhen. Repression of MS3 Gene Expression During Hmba Induced Differentiation of AN Embryonal Carcinoma Cell Line,B7-2 In vitro. Zoological Research, 1986, 7(4): 325-330.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1065) PDF downloads(534) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint