Developmental expression of three <em>prmt</em> genes in <em>Xenopus</em>

Cheng-Dong Wang; Xiao-Fang Guo; Thomas Chi Bun Wong; Hui Wang; Xu-Feng Qi; Dong-Qing Cai; Yi Deng; Hui Zhao

doi:10.24272/j.issn.2095-8137.2018.064

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Volume 40 Issue 2

Mar. 2019

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Article Navigation > Zoological Research > 2019 > 40(2): 102-107

Cheng-Dong Wang, Xiao-Fang Guo, Thomas Chi Bun Wong, Hui Wang, Xu-Feng Qi, Dong-Qing Cai, Yi Deng, Hui Zhao. Developmental expression of three prmt genes in Xenopus. Zoological Research, 2019, 40(2): 102-107. doi: 10.24272/j.issn.2095-8137.2018.064

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Cheng-Dong Wang, Xiao-Fang Guo, Thomas Chi Bun Wong, Hui Wang, Xu-Feng Qi, Dong-Qing Cai, Yi Deng, Hui Zhao. Developmental expression of three prmt genes in Xenopus. Zoological Research, 2019, 40(2): 102-107. doi: 10.24272/j.issn.2095-8137.2018.064

Developmental expression of three prmt genes in Xenopus

doi: 10.24272/j.issn.2095-8137.2018.064

1 Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China2 Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou Guangdong 510632, China3 Guangdong Provincial Key Laboratory of Cell Microenvironment, Department of Biology, South University of Science and Technology of China, Shenzhen Guangdong 518055, China4 Kunming Institute of Zoology, Chinese Academy of Sciences-The Chinese University of Hong Kong Joint Laboratory of Bioresources and Molecular Research of Common Diseases, Hong Kong SAR, China5 Department of Developmental & Regenerative Biology, College of Life Science and Technology, Jinan University, Guangzhou Guangdong 510632, China

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Corresponding author: Hui Zhao,E-mail:zhaohui@cuhk.edu.hk
Publish Date: 2019-03-18

Abstract

Protein arginine methyltransferases (PRMTs) are involved in many cellular processes via the arginine methylation of histone or non-histone proteins. We examined the expression patterns of prmt4, prmt7, and prmt9 during embryogenesis in Xenopus using whole-mount in situ hybridization and quantitative reverse transcription polymerase chain reaction (RT-PCR). Xenopus prmt4 and prmt7 were expressed in the neural crest, brain, and spinal cord, and also detected in the eye, branchial arches, and heart at the tailbud stage. Specific prmt9 signals were not detected in Xenopus embryos until the late tailbud stage when weak expression was observed in the branchial arches. Quantitative RT-PCR indicated that the expressions of prmt4 and prmt7 were up-regulated during the neurula stage, whereas prmt9 maintained its low expression until the late tailbud stage, consistent with the whole-mount in situ hybridization results. Thus, the developmental expression patterns of these three prmt genes in Xenopus embryos provide a basis for further functional study of such genes.
- Xenopus,
- prmt,
- Arginine methylation,
- Gene expression pattern
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Developmental expression of three prmt genes in Xenopus

doi: 10.24272/j.issn.2095-8137.2018.064

1 Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China2 Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou Guangdong 510632, China3 Guangdong Provincial Key Laboratory of Cell Microenvironment, Department of Biology, South University of Science and Technology of China, Shenzhen Guangdong 518055, China4 Kunming Institute of Zoology, Chinese Academy of Sciences-The Chinese University of Hong Kong Joint Laboratory of Bioresources and Molecular Research of Common Diseases, Hong Kong SAR, China5 Department of Developmental & Regenerative Biology, College of Life Science and Technology, Jinan University, Guangzhou Guangdong 510632, China

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Corresponding author: Hui Zhao,E-mail:zhaohui@cuhk.edu.hk

Publish Date: 2019-03-18

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Abstract: Protein arginine methyltransferases (PRMTs) are involved in many cellular processes via the arginine methylation of histone or non-histone proteins. We examined the expression patterns of prmt4, prmt7, and prmt9 during embryogenesis in Xenopus using whole-mount in situ hybridization and quantitative reverse transcription polymerase chain reaction (RT-PCR). Xenopus prmt4 and prmt7 were expressed in the neural crest, brain, and spinal cord, and also detected in the eye, branchial arches, and heart at the tailbud stage. Specific prmt9 signals were not detected in Xenopus embryos until the late tailbud stage when weak expression was observed in the branchial arches. Quantitative RT-PCR indicated that the expressions of prmt4 and prmt7 were up-regulated during the neurula stage, whereas prmt9 maintained its low expression until the late tailbud stage, consistent with the whole-mount in situ hybridization results. Thus, the developmental expression patterns of these three prmt genes in Xenopus embryos provide a basis for further functional study of such genes.

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