Volume 39 Issue 5
Sep.  2018
Turn off MathJax
Article Contents
Wen-Hui Nie, Jin-Huan Wang, Wei-Ting Su, Yu Hu, Shui-Wang He, Xue-Long Jiang, Kai He. Species identification of crested gibbons (Nomascus) in captivity in China using karyotyping- and PCR-based approaches. Zoological Research, 2018, 39(5): 356-363. doi: 10.24272/j.issn.2095-8137.2018.036
Citation: Wen-Hui Nie, Jin-Huan Wang, Wei-Ting Su, Yu Hu, Shui-Wang He, Xue-Long Jiang, Kai He. Species identification of crested gibbons (Nomascus) in captivity in China using karyotyping- and PCR-based approaches. Zoological Research, 2018, 39(5): 356-363. doi: 10.24272/j.issn.2095-8137.2018.036

Species identification of crested gibbons (Nomascus) in captivity in China using karyotyping- and PCR-based approaches

doi: 10.24272/j.issn.2095-8137.2018.036
Funds:   
More Information
  • Corresponding author: Wen-Hui Nie,Xue-Long Jiang,Kai He,E-mail:whnie@mail.kiz.ac.cn; jiangxl@mail.kiz.ac.cn; hekai@mail.kiz.ac.cn
  • Publish Date: 2018-09-18
  • Gibbons and siamangs (Hylobatidae) are well-known for their rapid chromosomal evolution, which has resulted in high speciation rate within the family. On the other hand, distinct karyotypes do not prevent speciation, allowing interbreeding between individuals in captivity, and the unwanted hybrids are ethically problematic as all gibbon species are endangered or critically endangered. Thus, accurate species identification is crucial for captive breeding, particularly in China where studbooks are unavailable. Identification based on external morphology is difficult, especially for hybrids, because species are usually similar in appearance. In this study, we employed G-banding karyotyping and fluorescence in situ hybridization (FISH) as well as a PCR-based approach to examine karyotypic characteristics and identify crested gibbons of the genus Nomascus from zoos and nature reserves in China. We characterized and identified five karyotypes from 21 individuals of Nomascus. Using karyotypes and mitochondrial and nuclear genes, we identified three purebred species and three hybrids, including one F2 hybrid between N. gabriellae and N. siki. Our results also supported that N. leucogenys and N. siki shared the same inversion on chromosome 7, which resolves arguments from previous studies. Our results demonstrated that both karyotyping and DNA-based approaches were suitable for identifying purebred species, though neither was ideal for hybrid identification. The advantages and disadvantages of both approaches are discussed. Our results further highlight the importance of animal ethics and welfare, which are critical for endangered species in captivity.
  • loading
  • Relative Articles

    [1] Jing CHE, Kai WANG. AmphibiaChina: an online database of Chinese Amphibians. Zoological Research, 2016, 37(1): 57-59.  doi: 10.13918/j.issn.2095-8137.2016.1.57
    [2] Zhen-Hua GUAN, Bei HUANG, Wen-He NING, Qing-Yong NI, Xue-Long JIANG. Proximity association in polygynous western black crested gibbons (Nomascus concolor jingdongensis): network structure and seasonality. Zoological Research, 2013, 34(E1): 561-E.  doi: 10.3724/SP.J.1141.2013.E01E01
    [3] . 《动物学研究》2013年34卷第2期全本PDF. Zoological Research, 2013, 34(2): 0-0.
    [4] CHENG Lei, CHANG Yu-Mei, LU Cui-Yun, CAO Ding-Cheng, SUN Xiao-Wen. DNA barcoding and species and subspecies classification within genus Carassius. Zoological Research, 2012, 33(5): 463-472.  doi: 10.3724/SP.J.1141.2012.05463
    [5] LI Guo-Song, YANG Xian-Ming, ZHANG Hong-Yu, LI Wei. Population and distribution of western black crested gibbon (Nomascus concolor) at Ailao Mountain, Xinping, Yunnan. Zoological Research, 2011, 32(6): 675-683.  doi: 10.3724/SP.J.1141.2011.06675
    [6] Yu guo-yu, ZHANG Hong-Yun, ZHANG Yong, JIANG Ping, Lee Wen-Hui, Zhang Yun. Expression and identification of Bm-TFF2 in Pichia pastoris. Zoological Research, 2010, 31(6): 565-569.  doi: 10.3724/SP.J.1141.2010.06565
    [7] CHEN Jun, LI Qi, KONG Ling-Feng, ZHENG Xiao-Dong, YU Rui-Hai. COI-based DNA Barcoding in Tapetinae Species (Mollusca, Bivalvia, Veneridae) along the Coast of CHINA. Zoological Research, 2010, 31(4): 345-352.  doi: 10.3724/SP.J.1141.2010.04345
    [8] CUI Shu-fang, QU Xiu-hua, HAN Shi-zhong, YU Chen-lin, SUN Wei, TANG Qiu. Application of Dual-color Fluorescence Hybridization Chip Technique in the Monitoring of the Genetic Quality of Inbred Mice Strains. Zoological Research, 2008, 29(1): 10-16.
    [9] ZHOU Jiang, CHAN Bosco Pui Lok, WEI Fu-wen. Responses to Inter-group Encounters of the Hainan Gibbon Nomascus hainanus. Zoological Research, 2008, 29(6): 667-673.  doi: 10.3724/SP.J.1141.2008.06667
    [10] SUN Guo-zheng, FAN Peng-fei, NI Qing-yong, ANG Jian-ling, JIANG Xue-long, *. Habitat Structure of Black Crested Gibbons (Nomascus concolor) in Pinghe, Ailao Mountains, Yunnan. Zoological Research, 2007, 28(4): 374-382.
    [11] Que Tengcheng, HU Yanling, Zhang Caichang, Huang Chengming, Mengxiuju. Observations on the Hybrid F of Trachypthecus leucocephalus and T. francioisi and Its Offspring. Zoological Research, 2007, 28(3): 225-230.
    [12] FAN Peng-fei, JIANG Xue-long , *, LIU Chang-ming, LUO Wen-shou. Polygynous Mating System and Behavioural Reason of Black Crested Gibbon (Nomascus concolor jingdongensis) at Dazhaizi, Mt. Wuliang, Yunnan, China. Zoological Research, 2006, 27(2): 216-220.
    [13] ZHOU Dao-lei, ZHAO Ya-jun*, LI Bao-ming*, SHI Zheng-xiang. Effect of Transport Space Allowance on Piglets' Behaviour and Performance. Zoological Research, 2006, 27(3): 261-268.
    [14] WENG You-Zhu, HUANG Wei-Quan, FANG Yong-Qiang, YAO Bing, SUN Lan. Study on GnRH Receptor (GnRHR) mRNA In situ Hybridization in The Nervous System,Hatschek's Pit and Gonad of Amphioxus,Branchiostoma belcheri. Zoological Research, 2000, 21(6): 437-441.
    [15] YANG Zhao-qing, ZUO Yang-xian, CHEN Xin-wen, DING Bo, LUO Jing, ZHANG Ya-ping. 18S rRNA Gene of Sarcocystis hominis Cyst From Water Buffalo and Cattle. Zoological Research, 2000, 21(2): 133-138.
    [16] QUAN Jie-xia, DAI Ji-xun. Current and Future Application of Fluorescence In situ Hybridization (Fish) Techniques To Fish Genetics:A Review. Zoological Research, 1999, 20(3): 225-229.
    [17] ZHANG Yuan-qiang, WU Sheng-xi, XU Ruo-jun, ZHANG Shu-hua. Studies on Immunohistochemistry and In situ Hybridization of Calcitonin Gene-Related Peptide and Its mRNA In Lungs of Macaca mulatta. Zoological Research, 1998, 19(2): 107-113.
    [18] GUI Jian-fang, LIANG Shao-chang, ZHU Lan-fei, SUN Jian-min, JIANG Yi-gui. Cytogenetic Analysis of Developmental Difference in Hybrid embryos Between Reciprocal Crosses in Distant Hybridization of Fishes. Zoological Research, 1993, 14(2): 171-177.
    [19] Colin P.Groves, WANG Ying-xiang. The Gibbons of The Subgenus Nomascus (Primates,Mammalia). Zoological Research, 1990, 11(2): 147-154.
    [20] YU Min-shu, XUE Jin-lun, HUANG Min-jun. Effect of Prostaglandin E2 and F2α on the Level of Haptoglobin in Rabbit. Zoological Research, 1988, 9(zk): 15-22.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (600) PDF downloads(441) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return