Volume 38 Issue 3
May  2017
Turn off MathJax
Article Contents

Lei Guo, Yan-Cui Wang, Jun-Jie Mei, Ruo-Tong Ning, Jing-Jing Wang, Jia-Qi Li, Xi Wang, Hui-Wen Zheng, Hai-Tao Fan, Long-Ding Liu. Pulmonary immune cells and inflammatory cytokine dysregulation are associated with mortality of IL-1R1-/-mice infected with influenza virus (H1N1). Zoological Research, 2017, 38(3): 146-154. doi: 10.24272/j.issn.2095-8137.2017.035
Citation: Lei Guo, Yan-Cui Wang, Jun-Jie Mei, Ruo-Tong Ning, Jing-Jing Wang, Jia-Qi Li, Xi Wang, Hui-Wen Zheng, Hai-Tao Fan, Long-Ding Liu. Pulmonary immune cells and inflammatory cytokine dysregulation are associated with mortality of IL-1R1-/-mice infected with influenza virus (H1N1). Zoological Research, 2017, 38(3): 146-154. doi: 10.24272/j.issn.2095-8137.2017.035

Pulmonary immune cells and inflammatory cytokine dysregulation are associated with mortality of IL-1R1-/-mice infected with influenza virus (H1N1)

doi: 10.24272/j.issn.2095-8137.2017.035
Funds:  This work was supported by the National Natural Science Foundation of China (31300143, 31570900), the Applied Basic Research Foundation of Yunnan Province, China (2015FB139), and the Chinese Academy of Medical Science (CAMS) Innovation Fund for Medical Sciences (2016-I2M-1-014)
More Information
  • Corresponding author: Long-Ding Liu,E-mail:liuld@imbcams.com.cn
  • Received Date: 2017-02-28
  • Rev Recd Date: 2017-04-19
  • Publish Date: 2017-05-18
  • Respirovirus infection can cause viral pneumonia and acute lung injury (ALI). The interleukin-1 (IL-1) family consists of proinflammatory cytokines that play essential roles in regulating immune and inflammatory responses in vivo. IL-1 signaling is associated with protection against respiratory influenza virus infection by mediation of the pulmonary anti-viral immune response and inflammation. We analyzed the infiltration lung immune leukocytes and cytokines that contribute to inflammatory lung pathology and mortality of fatal H1N1 virus-infected IL-1 receptor 1 (IL-1R1) deficient mice. Results showed that early innate immune cells and cytokine/chemokine dysregulation were observed with significantly decreased neutrophil infiltration and IL-6, TNF-α, G-CSF, KC, and MIP-2 cytokine levels in the bronchoalveolar lavage fluid of infected IL-1R1-/- mice in comparison with that of wild type infected mice. The adaptive immune response against the H1N1 virus in IL-1R1-/- mice was impaired with downregulated anti-viral Th1 cell, CD8+ cell, and antibody functions, which contributes to attenuated viral clearance. Histological analysis revealed reduced lung inflammation during early infection but severe lung pathology in late infection in IL-1R1-/- mice compared with that in WT infected mice. Moreover, the infected IL-1R1-/- mice showed markedly reduced neutrophil generation in bone marrow and neutrophil recruitment to the inflamed lung. Together, these results suggest that IL-1 signaling is associated with pulmonary anti-influenza immune response and inflammatory lung injury, particularly via the influence on neutrophil mobilization and inflammatory cytokine/chemokine production.
  • 加载中
  • [1] Bendall LJ,Bradstock KF.2014.G-CSF:from granulopoietic stimulant to bone marrow stem cell mobilizing agent.Cytokine&Growth Factor Reviews,25(4):355-367.
    [2] Besnard AG,Togbe D,Couillin I,Tan ZM,Zheng SG,Erard F,Le Bert M,Quesniaux V,Ryffel B.2012.Inflammasome-IL-1-Th17 response in allergic lung inflammation.Journal of Molecular Cell Biology,4(1):3-10.
    [3] Braciale TJ,Sun J,Kim TS.2012.Regulating the adaptive immune response to respiratory virus infection.Nature Reviews Immunology,12(4):295-305.
    [4] Coates BM,Staricha KL,Wiese KM,Ridge KM.2015.Influenza a virus infection,innate immunity,and childhood.JAMA Pediatrics,169(10):956-963.
    [5] Damjanovic D,Small CL,Jeyananthan M,McCormick S,Xing Z.2012.Immunopathology in influenza virus infection:uncoupling the friend from foe.Clinical Immunology,144(1):57-69.
    [6] Damjanovic D,Divangahi M,Kugathasan K,Small CL,Zganiacz A,Brown EG,Hogaboam CM,Gauldie J,Xing Z.2011.Negative regulation of lung inflammation and immunopathology by TNF-α during acute influenza infection.American Journal of Pathology,179(6):2963-2976.
    [7] Dienz O,Rud JG,Eaton SM,Lanthier PA,Burg E,Drew A,Bunn J,Suratt BT,Haynes L,Rincon M.2012.Essential role of IL-6 in protection against H1N1 influenza virus by promoting neutrophil survival in the lung.Mucosal Immunology,5(3):258-266.
    [8] Dinarello CA.2009.Immunological and inflammatory functions of the interleukin-1 family.Annual Review of Immunology,27(1):519-550.
    [9] Durrant DM,Robinette ML,Klein RS.2013.IL-1R1 is required for dendritic cell-mediated T cell reactivation within the CNS during West Nile virus encephalitis.The Journal of Experimental Medicine,210(3):503-516.
    [10] Furze RC,Rankin SM.2008.Neutrophil mobilization and clearance in the bone marrow.Immunology,125(3):281-288.
    [11] Greenbaum LA,Horowitz JB,Woods A,Pasqualini T,Reich EP,Bottomly K.1988.Autocrine growth of CD4+T cells.Differential effects of IL-1 on helper and inflammatory T cells.The Journal of Immunology,140(5):1555-1560.
    [12] Kohlmeier JE,Woodland DL.2009.Immunity to respiratory viruses.Annual Review of Immunology,27(1):61-82.
    [13] Kolaczkowska E,Kubes P.2013.Neutrophil recruitment and function in health and inflammation.Nature Reviews Immunology,13(3):159-175.
    [14] Leliefeld PHC,Koenderman L,Pillay J.2015.How neutrophils shape adaptive immune responses.Frontiers in Immunology,6:471.
    [15] Lichtman AH,Chin J,Schmidt JA,Abbas AK.1988.Role of interleukin 1 in the activation of T lymphocytes.Proceedings of the National Academy of Sciences of the United States of America,85(24):9699-9703.
    [16] Mantovani A,Cassatella MA,Costantini C,Jaillon S.2011.Neutrophils in the activation and regulation of innate and adaptive immunity.Nature Reviews Immunology,11(8):519-531.
    [17] O'Garra A,Murphy K.1994.Role of cytokines in determining T-lymphocyte function.Current Opinion in Immunology,6(3):458-466.
    [18] Olson TS,Ley K.2002.Chemokines and chemokine receptors in leukocyte trafficking.American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,283(1):R7-R28.
    [19] Sanders CJ,Doherty PC,Thomas PG.2011.Respiratory epithelial cells in innate immunity to influenza virus infection.Cell and Tissue Research,343(1):13-21.
    [20] Satoskar AR,Okano M,Connaughton S,Raisanen-Sokolwski A,David JR,Labow M.1998.Enhanced Th2-like responses in IL-1 type 1 receptordeficient mice.European Journal of Immunology,28(7):2066-2074.
    [21] Schmitz N,Kurrer M,Bachmann MF,Kopf M.2005.Interleukin-1 is responsible for acute lung immunopathology but increases survival of respiratory influenza virus infection.Journal of Virology,79(10):6441-6448.
    [22] Strieter RM,Belperio JA,Keane MP.2003.Host innate defenses in the lung:the role of cytokines.Current Opinion in Infectious Diseases,16(3):193-198.
    [23] Strydom N,Rankin SM.2013.Regulation of circulating neutrophil numbers under homeostasis and in disease.Journal of Innate Immunity,5(4):304-314.
    [24] Szretter KJ,Gangappa S,Lu XH,Smith C,Shieh WJ,Zaki SR,Sambhara S,Tumpey TM,Katz JM.2007.Role of host cytokine responses in the pathogenesis of avian H5N1 influenza viruses in mice.Journal of Virology,81(6):2736-2744.
    [25] Tripathi S,White MR,Hartshorn KL.2015.The amazing innate immune response to influenza A virus infection.Innate Immunity,21(1):73-98.
    [26] Weber A,Wasiliew P,Kracht M.2010.Interleukin-1(IL-1) pathway.Science Signaling,3(105):cm1.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1216) PDF downloads(692) Cited by()

Related
Proportional views

Pulmonary immune cells and inflammatory cytokine dysregulation are associated with mortality of IL-1R1-/-mice infected with influenza virus (H1N1)

doi: 10.24272/j.issn.2095-8137.2017.035
Funds:  This work was supported by the National Natural Science Foundation of China (31300143, 31570900), the Applied Basic Research Foundation of Yunnan Province, China (2015FB139), and the Chinese Academy of Medical Science (CAMS) Innovation Fund for Medical Sciences (2016-I2M-1-014)
    Corresponding author: Long-Ding Liu,E-mail:liuld@imbcams.com.cn

Abstract: Respirovirus infection can cause viral pneumonia and acute lung injury (ALI). The interleukin-1 (IL-1) family consists of proinflammatory cytokines that play essential roles in regulating immune and inflammatory responses in vivo. IL-1 signaling is associated with protection against respiratory influenza virus infection by mediation of the pulmonary anti-viral immune response and inflammation. We analyzed the infiltration lung immune leukocytes and cytokines that contribute to inflammatory lung pathology and mortality of fatal H1N1 virus-infected IL-1 receptor 1 (IL-1R1) deficient mice. Results showed that early innate immune cells and cytokine/chemokine dysregulation were observed with significantly decreased neutrophil infiltration and IL-6, TNF-α, G-CSF, KC, and MIP-2 cytokine levels in the bronchoalveolar lavage fluid of infected IL-1R1-/- mice in comparison with that of wild type infected mice. The adaptive immune response against the H1N1 virus in IL-1R1-/- mice was impaired with downregulated anti-viral Th1 cell, CD8+ cell, and antibody functions, which contributes to attenuated viral clearance. Histological analysis revealed reduced lung inflammation during early infection but severe lung pathology in late infection in IL-1R1-/- mice compared with that in WT infected mice. Moreover, the infected IL-1R1-/- mice showed markedly reduced neutrophil generation in bone marrow and neutrophil recruitment to the inflamed lung. Together, these results suggest that IL-1 signaling is associated with pulmonary anti-influenza immune response and inflammatory lung injury, particularly via the influence on neutrophil mobilization and inflammatory cytokine/chemokine production.

Lei Guo, Yan-Cui Wang, Jun-Jie Mei, Ruo-Tong Ning, Jing-Jing Wang, Jia-Qi Li, Xi Wang, Hui-Wen Zheng, Hai-Tao Fan, Long-Ding Liu. Pulmonary immune cells and inflammatory cytokine dysregulation are associated with mortality of IL-1R1-/-mice infected with influenza virus (H1N1). Zoological Research, 2017, 38(3): 146-154. doi: 10.24272/j.issn.2095-8137.2017.035
Citation: Lei Guo, Yan-Cui Wang, Jun-Jie Mei, Ruo-Tong Ning, Jing-Jing Wang, Jia-Qi Li, Xi Wang, Hui-Wen Zheng, Hai-Tao Fan, Long-Ding Liu. Pulmonary immune cells and inflammatory cytokine dysregulation are associated with mortality of IL-1R1-/-mice infected with influenza virus (H1N1). Zoological Research, 2017, 38(3): 146-154. doi: 10.24272/j.issn.2095-8137.2017.035
Reference (26)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return