School of Agriculture and Natural Resources, University of Phayao, Phayao 56000, Thailand
2 Southeast Asia Biodiversity Research Institute (CAS-SEABRI), Chinese Academy of Sciences, Yezin Nay Pyi Taw 05282, Myanmar
3 State Key Laboratory for Conservation and Utilization of Bio-resource in Yunnan, Yunnan University, Kunming Yunnan 650091, China
4 State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China
5 Wildlife Forensics Science Service Centre, Kunming Yunnan 650203, China
6 Guizhou Academy of Testing and Analysis, Guizhou Academy of Sciences, Guiyang Guizhou 550002, China
This study was supported by the National Key Research and Development Program (2016YFC1200705), Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences (Y4ZK111B01: 2015CASEABRI002), Ministry of Science and Technology of China (2012FY110800), Chinese Academy of Sciences (KFZD-SW-208), and Yunnan Provincial Science and Technology Department
In this study, we sequenced the complete mitochondrial genome (mitogenome) of the Thai Red Junglefowl (RJF; Gallus gallus) using the next-generation sequencing (NGS) platform of the Ion Torrent PGM. Samples were taken from Mae Wang District, Chiang Mai Province, northern Thailand. Our data showed the complete mitogenome to be 16 785 bp in length, composed by 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one control region. The genome nucleotide composition was 30.3% A, 23.7% T, 32.5% C, and 13.5% G, resulting in a high percentage of A+T (50.4%). Phylogenetic analysis revealed that the mitogenome belonged to haplogroup X, whereas those of all domestic chickens belong to haplogroups A to G. This newly released mitogenome sequence will advance further evolutionary and population genetics study of the RJF and domestic chicken. The availability of the G. gallus mitogenome will also contribute to further conservation genetics research of a unique species, listed as ‘data deficient’ in Thailand.