Advanced Search
Volume 38 Issue 2
Mar.  2017
Turn off MathJax
Article Contents
Article Navigation >  Zoological Research  > 2017  >  38(2): 103-109
Xiu-Xiu Liu, Cheng-Yuan Wang, Chun Luo, Jun-Qing Sheng, Di Wu, Bei-Juan Hu, Jun-Hua Wang, Yi-Jiang Hong. Characterization of cyclophilin D in freshwater pearl mussel (Hyriopsis schlegelii). Zoological Research, 2017, 38(2): 103-109. doi: 10.24272/j.issn.2095-8137.2017.018
Citation: Xiu-Xiu Liu, Cheng-Yuan Wang, Chun Luo, Jun-Qing Sheng, Di Wu, Bei-Juan Hu, Jun-Hua Wang, Yi-Jiang Hong. Characterization of cyclophilin D in freshwater pearl mussel (Hyriopsis schlegelii). Zoological Research, 2017, 38(2): 103-109. doi: 10.24272/j.issn.2095-8137.2017.018
shu

Characterization of cyclophilin D in freshwater pearl mussel (Hyriopsis schlegelii)

doi: 10.24272/j.issn.2095-8137.2017.018

  • 1 School of Life Sciences, Nanchang University, Nanchang Jiangxi 330031, China;

  • 2 Key Laboratory of Aquatic Animals Resources and Utilization of Jiangxi, Nanchang University, Nanchang Jiangxi 330031, China

Funds:  This study was supported by the National Natural Science Foundation of China (31660337), Special Aquatic Products Industry Technology System of Jiangxi (JXARS-10), Scientific and Technological Program of Jiangxi Province (KJLD12001, 20152ACF60013 and 150166), and Natural Science Foundation of Jiangxi Province (20122BAB204016)
More Information
  • Corresponding author: Yi-Jiang Hong
  • Received Date: 2016-11-07
  • Rev Recd Date: 2017-03-07
  • Publish Date: 2017-03-18
    Abstract
  • Cyclophilin D (referred to as HsCypD) was obtained from the freshwater pearl mussel (Hyriopsis schlegelii). The full-length cDNA was 2 671 bp, encoding a protein consisting of 367 amino acids. HsCypD was determined to be a hydrophilic intracellular protein with 10 phosphorylation sites and four tetratricopeptide repeat (TPR) domains, but no signal peptide. The core sequence region YKGCIFHRIIKDFMVQGG is highly conserved in vertebrates and invertebrates. Phylogenetic tree analysis indicated that CypD from all species had a common origin, and HsCypD had the closest phylogenetic relationship with CypD from Lottia gigantea. The constitutive mRNA expression levels of HsCypD exhibited tissue-specific patterns, with the highest level detected in the intestines, followed by the gonads, and the lowest expression found in the hemocytes.
    • FullText(HTML)
  • loading
    • References(33)
  • [1]
    Basso E, Fante L, Fowlkes J, Petronilli V, Forte MA, Bernardi P. 2005. Properties of the permeability transition pore in mitochondria devoid of Cyclophilin D. Journal of Biological Chemistry, 280(19):18558-18561.
    [2]
    Blackburn EA, Wear MA, Landré V, Narayan V, Ning J, Erman B, Ball KL, Walkinshaw MD. 2015. Cyclophilin40 isomerase activity is regulated by a temperature-dependent allosteric interaction with Hsp90. Bioscience Reports, 35(5):e00258.
    [3]
    Borgese N, Aggujaro D, Carrera P, Pietrini G, Bassetti M. 1996. A role for N-myristoylation in protein targeting:NADH-cytochrome b5 reductase requires myristic acid for association with outer mitochondrial but not ER membranes. The Journal of Cell Biology, 135(6):1501-1513.
    [4]
    Chen LL, Mu CK, Zhao JM, Wang CL. 2011. Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum. Fish & Shellfish Immunology, 31(6):1218-1223.
    [5]
    Cheng HC, Matsuura I, Wang JH. 1993. In vitro substrate specificity of protein tyrosine kinases. In:Khandelwal RL, Wang JH. Reversible Protein Phosphorylation in Cell Regulation. US:Springer, 103-112.
    [6]
    Danielson PE, Forss-Petter S, Brow MA, Calavetta L, Douglass J, Milner RJ, Sutcliffe JG. 1988. pl Bl5:a cDNA clone of the rat mRNA encoding cyclophilin. DNA, 7(4):261-267.
    [7]
    Feng WH, Xin Y. 2013. Progress in research on cyclophilin A and malignant tumors. Tumor, 33(10):928-934. (in Chinese)
    [8]
    Forte M, Bernardi P. 2006. The permeability transition and BCL-2 family proteins in apoptosis:co-conspirators or independent agents? Cell Death & Differentiation, 13(8):1287-1290.
    [9]
    Halestrap AP, Kerr PM, Javadov S, Woodfield KY. 1998. Elucidating the molecular mechanism of the permeability transition pore and its role in reperfusion injury of the heart. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1366(1-2):79-94.
    [10]
    Hausenloy DJ, Boston-Griffiths EA, Yellon DM. 2012. Cyclosporin A and cardioprotection:from investigative tool to therapeutic agent. British Journal of Pharmacology, 165(5):1235-1245.
    [11]
    Hunter DR, Haworth RA. 1979. The Ca2+-induced membrane transition in mitochondria:I. The protective mechanisms. Archives of Biochemistry and Biophysics, 195(2):453-459.
    [12]
    Kyte J, Doolittle RF. 1982. A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology, 157(1):105-132.
    [13]
    Lee YK, Hong CB, Suh Y, Lee IK. 2002. A cDNA clone for cyclophilin from Griffithsia japonica and phylogenetic analysis of cyclophilins. Molecules and Cells, 13(1):12-20.
    [14]
    Li YM, Johnson N, Capano M, Edwards M, Crompton M. 2004. Cyclophilin-D promotes the mitochondrial permeability transition but has opposite effects on apoptosis and necrosis. Biochemical Journal, 383(1):101-109.
    [15]
    Lin DT, Lechleiter JD. 2002. Mitochondrial targeted cyclophilin D protects cells from cell death by peptidyl prolyl isomerization. Journal of Biological Chemistry, 277(34):31134-31141.
    [16]
    Luo C, Xu L, He J, Shi JW, Sheng JQ, Peng K, Wang JH, Huang B, Hong YJ. 2015. The expression of cyclophilin a (CyPA) in Hyriopsis schlegelii in response to pathogens and its effect on the growth of hela cells. Acta Hydrobiologica Sinica, 39(3):475-481. (in Chinese)
    [17]
    Marais RM, Hsuan JJ, McGuigan C, Wynne J, Treisman R. 1992. Casein kinase II phosphorylation increases the rate of serum response factor-binding site exchange. The EMBO Journal, 11(1):97-105.
    [18]
    Maurer-Stroh S, Eisenhaber B, Eisenhaber F. 2002. N-terminal N-myristoylation of proteins:refinement of the sequence motif and its taxon-specific differences. Journal of Molecular Biology, 317(4):523-540.
    [19]
    Mikol V, Kallen J, Walkinshaw MD. 1994. X-ray structure of a cyclophilin B/cyclosporin complex:comparison with cyclophilin A and delineation of its calcineurin-binding domain. Proceedings of the National Academy of Sciences of the United States of America, 91(11):5183-5186.
    [20]
    Nishizuka Y. 1984. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature, 308(5961):693-698.
    [21]
    Ottiger M, Zerbe O, Güntert P, Wüthrich K. 1997. The NMR solution conformation of unligated human cyclophilin A. Journal of Molecular Biology, 272(1):64-81.
    [22]
    Qiu LH, Jiang SG, Huang JH, Wang WF, Zhu CY, Su TF. 2009. Molecular cloning and mRNA expression of cyclophilin A gene in black tiger shrimp (Penaeus monodon). Fish & Shellfish Immunology, 26(1):115-121.
    [23]
    Schmittgen TD, Livak KJ. 2008. Analyzing real-time PCR data by the comparative CT method. Nature Protocols, 3(6):1101-1108.
    [24]
    Schubert A, Grimm S. 2004. Cyclophilin D, a component of the permeability transition-pore, is an apoptosis repressor. Cancer Research, 64(1):85-93.
    [25]
    Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4:molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24(8):1596-1599.
    [26]
    Tavecchio M, Lisanti S, Lam A, Ghosh JC, Martin NM, O'Connell M, Weeraratna AT, Kossenkov AV, Showe LC, Altieri DC. 2013. Cyclophilin D extramitochondrial signaling controls cell cycle progression and chemokine-directed cell motility. Journal of Biological Chemistry, 288(8):5553-5561.
    [27]
    Taylor P, Dornan J, Carrello A, Minchin RF, Ratajczak T, Walkinshaw MD. 2001. Two structures of cyclophilin 40:folding and fidelity in the TPR domains. Structure, 9(5):431-438.
    [28]
    Thomas B, Banerjee R, Starkova NN, Zhang SF, Calingasan NY, Yang LC, Wille E, Lorenzo BJ, Ho DJ, Beal MF, Starkov A. 2012. Mitochondrial permeability transition pore component cyclophilin D distinguishes nigrostriatal dopaminergic death paradigms in the MPTP mouse model of Parkinson's disease. Antioxidants & Redox Signaling, 16(9):855-868.
    [29]
    Wang CY, Sheng JQ, Hong YJ, Peng K, Wang JH, Wu D, Shi JW, Hu BJ. 2016. Molecular characterization and expression of metallothionein from freshwater pearl mussel, Hyriopsis schlegelii. Bioscience, Biotechnology, and Biochemistry, 80(7):1327-1335.
    [30]
    Wang QF, Wang FQ, Ge HL. 2009. The progress of cyclophilin A in cancer research. International Journal of Immunology, 32(2):99-103. (in Chinese)
    [31]
    Watashi K, Ishii N, Hijikata M, Inoue D, Murata T, Miyanari Y, Shimotohno K. 2005. Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase. Molecular Cell, 19(1):111-122.
    [32]
    Xie K, Xu L, Sheng JQ, Zeng LG, Wang JH, Hong YJ. 2011. The full-length cDNA library of hemocyte induced by Aeromonas hydrophila and molecular characteristics of cyclophilin a from Hyriopsis schlegelii. Acta Hydrobiologica Sinica, 35(5):783-789. (in Chinese)
    [33]
    Young JC, Obermann WMJ, Hartl FU. 1998. Specific binding of tetratricopeptide repeat proteins to the C-terminal 12-kDa domain of hsp90. Journal of Biological Chemistry, 273(29):18007-18010.
    • Relative Articles(20)

  • Relative Articles

    [1] Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 2016, 37(2): 96-102.  doi: 10.13918/j.issn.2095-8137.2016.2.96
    [2] Jin-Hua WANG, Liang MIAO, Ming-Yun LI, Xiao-Fei GUO, Na PAN, Ying-Ying CHEN, Liang ZHAO. Cloning the Dmrt1 and DmrtA2 genes of ayu (Plecoglossus altivelis) and mapping their expression in adult, larval, and embryonic stages. Zoological Research, 2014, 35(2): 99-107.  doi: 10.11813/j.issn.0254-5853.2014.2.099
    [3] Kou PENG, Cheng-Yuan WANG, Jun-Hua WANG, Jun-Qing SHENG, Jian-Wu SHI, Jian LI, Yi-Jiang HONG. Molecular cloning, sequence analysis, and cadmium stress-rated expression changes of BTG1 in freshwater pearl mussel (Hyriopsis schlegelii). Zoological Research, 2014, 35(5): 389-397.  doi: 10.13918/j.issn.2095-8137.2014.5.389
    [4] Cai-Yun WANG, Yun-Han MA, Da-Jian HE, Shi-Hua YANG. cDNA cloning and sequence analysis of pluripotency genes in tree shrews (Tupaia belangeri). Zoological Research, 2013, 34(2): 127-131.  doi: 10.3724/SP.J.1141.2013.02127
    [5] YANG Hui, HUANG Yuan. Analysis of the complete mitochondrial genome sequence of Pielomastax zhengi. Zoological Research, 2011, 32(4): 353-362.  doi: 10.3724/SP.J.1141.2011.04353
    [6] LIU Si-Qing, ZHANG Jia-Bo, TANG Qiong-Ying, LIU Huan-Zhang. Phylogenetic Relationships Among Cobitoidea Based on Mitochondrial ND4 and ND5 Gene Sequences. Zoological Research, 2010, 31(3): 221-229.
    [7] HUANG Yan, LIANG Xu-Fang*, WANG Lin, LI Guang-Zhao, Liu Xiu-Xia, YAO Yu. Molecular Characterization and Evolutional Analysis of Liportein Lipase (LPL) and Hepatic Lipase (HL) Gene in Chinese sturgeon and Other Six Freshwater Fishes. Zoological Research, 2010, 31(3): 239-249.  doi: 10.3724/SP.J.1141.2010.03239
    [8] GAO Jia, CHENG Chun-hua, HUANG Yuan. Analysis of Complete Mitochondrial Genome Sequence of Aeropus licenti Chang. Zoological Research, 2009, 30(6): 603-612.  doi: 10.3724/SP.J.1141.2009.06603
    [9] YU Ying, LIANG Xu-fang, LI Guan-gui, WANG Lin, LI Guang-zhao. Molecular Cloning and Analysis of Leptin Gene in Grass Carp (Ctenopharyngodon idellus) and Silver Carp (Hypophthalmichthys molitrix). Zoological Research, 2009, 30(5): 479-485.  doi: 10.3724/SP.J.1141.2009.05480
    [10] BAI Yin-ba-tu, LUO Fen-hua, HU Tian-yuan, HOU Yue, WU Ying-ji. Cloning of Sheep tnp2 Gene and Transcription Analysis of Round Spermatid Cells in the in vitro Co-culture System. Zoological Research, 2009, 30(3): 262-266.  doi: 10.3724/SP.J.1141.2009.03262
    [11] YANG Xiu-qin, GUO Li-juan, MA Jian-zhang, LIU Di. Cloning, Expression and Variation Analysis of the Wild Boar CAPN7 Gene. Zoological Research, 2009, 30(5): 503-508.  doi: 10.3724/SP.J.1141.2009.05503
    [12] LIU Wei-bin, GONG Cheng-liang, XUE Ren-yu, ZHOU Wen-lin, ZHU Xu-xian, CAO Guang-li *. Cloning and Analysis of Fhx/P25 Gene Promoter of Bombyx mori Fibroin Protein. Zoological Research, 2007, 28(1): 17-23.
    [13] WANG Qian, WU Xiao-bing* , YAN Peng. Sequence Variability Analysis of MHC Class Ⅱ DRB Alleles in Three Felines. Zoological Research, 2006, 27(2): 181-188.
    [14] ZHONG Shu-rong, JIN Yang, LI Dong-sheng, WANG Wan-yu, XIONG Yu-liang. Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C-type Lectin-like Protein Subunits from Daboia russellii siamensis. Zoological Research, 2005, 26(4): 337-343.
    [15] WANG Xian-hui, CHEN Bing, KANG Le. Clone and Sequence Analysis of cDNA Fragment of Heat Shock Protein 70 Gene in the Migratory Locust,Locusta migratoria. Zoological Research, 2003, 24(5): 349-354.
    [16] CHEN Dong-sheng, NIE Liu-wang, CHENG Shuang-huai, KAN Xian-zhao, WANG Chao-lin, XIE Wan-shu. Amplification and Sequencing of Sox Gene HMG-box in Alligator sinensis. Zoological Research, 2003, 24(2): 127-131.
    [17] WEI Qin, LI Rui, WANG Wan-yu, XIONG Yu-liang. Cloning and Sequence Analysis of cDNAs Encoding Four C-type Lectin-like Proteins from Snake Trimeresurus mucrosquamatus. Zoological Research, 2002, 23(4): 273-279.
    [18] BAI Jun-Jie, LAO Hai-Hua, YE Xing, LI Ying-Hua, LUO Jian-Ren. Molecular Cloning and Sequence Analysis of Insulin-like Growth Factor-Ⅰ cDNA from Bluntnose Bream (Megalobrama amblycephala). Zoological Research, 2001, 22(6): 502-506.
    [19] ZHA Hong-Guang, ZHANG Yun. Cloning of cDNAs Encoding Two Novel Phospholipase A[2]s from Bungarus fasciatus. Zoological Research, 2001, 22(5): 392-396.
    [20] XIAO Wu-han, WU Chun-hua, SU Bing, ZHANG Ya-ping, CUI Gui-hua. DNA Extracted From Formalin-Fixed Xenocypris yunnanensis and Sequence Analysis of Its Cytochrome B Gene. Zoological Research, 1997, 18(3): 242-252.
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索
    Get Citation
    PDF
    XML

    Article Metrics

    Article views (459) PDF downloads(958) Cited by()
    Proportional views
    Related

    Content

    For Authors

    About ZR

    Copyright © 2016 Editorial Office of ZOOLOGICAL RESEARCH

    滇ICP备05000723号-2滇公网安备 53010202000944号

    Supported by: Beijing Renhe Information Technology Co. Ltd  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return