Volume 37 Issue 4
Jul.  2016
Turn off MathJax
Article Contents
Lan-Zhen YAN, Bin SUN, Long-Bao LYU, Yu-Hua MA, Jia-Qi CHEN, Qing LIN, Ping ZHENG, Xu-Dong ZHAO. Early embryonic development and transplantation in tree shrews. Zoological Research, 2016, 37(4): 252-258. doi: 10.13918/j.issn.2095-8137.2016.4.252
Citation: Lan-Zhen YAN, Bin SUN, Long-Bao LYU, Yu-Hua MA, Jia-Qi CHEN, Qing LIN, Ping ZHENG, Xu-Dong ZHAO. Early embryonic development and transplantation in tree shrews. Zoological Research, 2016, 37(4): 252-258. doi: 10.13918/j.issn.2095-8137.2016.4.252

Early embryonic development and transplantation in tree shrews

doi: 10.13918/j.issn.2095-8137.2016.4.252
Funds:  This study was supported by the Breakthrough Project of Strategic Priority Program of the Chinese Academy of Sciences (XDB13000000)
More Information
  • Corresponding author: Xu-Dong ZHAO
  • Received Date: 2016-01-08
  • Rev Recd Date: 2016-03-05
  • Publish Date: 2016-07-18
  • As a novel experimental animal model, tree shrews have received increasing attention in recent years. Despite this, little is known in regards to the time phases of their embryonic development. In this study, surveillance systems were used to record the behavior and timing of copulations; embryos at different post-copulation stages were collected and cultured in vitro; and the developmental characteristics of both early-stage and in vitro cultured embryos were determined. A total of 163 females were collected following effective copulation, and 150 were used in either unilateral or bilateral oviduct embryo collections, with 307 embryos from 111 females obtained (conception rate=74%). Among them, 237 embryos were collected from 78 females, bilaterally, i.e., the average embryo number per female was 3.04; 172 fertilized eggs collected from 55 females, bilaterally, were cultured for 24-108 h in vitro for developmental observations; finally, 65 embryos from 23 bilateral cases and 70 embryos from 33 unilateral cases were used in embryo transplantation.
  • loading
  • [1]
    Cao J, Yang EB, Su JJ, Li Y, Chow P. 2003. The tree shrews:adjuncts and alternatives to primates as models for biomedical research. Journal of Medical Primatology, 32(2):123-130.
    Cao XM. 1990. Microscopic structure of ovary and ovarian activity of different seasons in tree shrew (Tupaia belangeri chinensis). Zoological Research, 11(1):17-25. (in Chinese)
    Chen JQ, Yu YM, Chen J, Wu YQ, Cheng GX. 2002. Observation of reproductive organs and oocyte's character of female tree shrew anesthetized. Acta Laboratorium Animalis Scientia Sinica, 10(4):230-232. (in Chinese)
    Collins PM, Tsang WN. 1987. Growth and reproductive development in the male tree shrew (Tupaia belangeri) from birth to sexual maturity. Biology of Reproduction, 37(2):261-267.
    Collins PM, Tsang WN, Lofts B. 1982. Anatomy and function of the repro-ductive tract in the captive male tree shrew (Tupaia belangeri). Biology of Reproduction, 26(1):169-182.
    Ding F, Zhou HL, Liu Y, Ma L, Su Y, Du L. 2007. Effects of glucose on development of ICR mouse embryos in vitro. Zoological Research, 28(5):501-506. (in Chinese)
    Fu WD, Sun YC, Suo L, Gao JM. 2005. Observation of estrous cycle and choice of optimal superovulation time in mice. Journal of Beijing Agricultural College, 20(2):19-21. (in Chinese)
    Hubrecht RC, Kirkwood J. 2010. The UFAW Handbook on the Care and Management of Laboratory and other Research Animals. 8th ed. Singapore:Robert Hubrecht and James Kirkwood Press, 262-275.
    Jiang QF, Kuang DX, Tong PF, Sun XM, Dai JJ. 2011. Scale breeding of tree shrews and the establishment of breeding population. Laboratory Animal Science, 28(6):35-38. (in Chinese)
    Johnson MH, Ziomek CA. 1981. The foundation of two distinct cell lineages within the mouse morula. Cell, 24(1):71-80.
    Li YJ, Ao H, Sun GJ, Zhao YZ. 2005. Study on the gene expression of different goat preimplantation embryos on given specific stage. Acta Veterinaria et Zootechnica Sinica, 36(12):1281-1285. (in Chinese)
    Ma YZ, Hu TM, Xia GL, Bou SG. 2007. Transgic ovine embryos production by means of pronuclear microinjection. Journal of Inner Mongolia University, 38(6):660-664. (in Chinese)
    Mercader A, Simón C, Galán A, Herrer R, Albert C, Remohi J, Pellicer A. 2001. An analysis of spontaneous hatching in a human endometrial epi-thelial coculture system:is assisted hatching justified?. Journal of Assisted Reproduction and Genetics, 18(6):315-319.
    Nagy A, Gertsenstein M, Vintersten K, Behringer R. 2003. Manipulating the Mouse Embryo:A Laboratory Manual. New York:Cold Spring Harbor Laboratory Press.
    Pang YQ, Feng Y, Sun XM, Liu L, Dai JJ, Xia XS. 2014. Research progress of tree shrew models of viral hepatitis and modeling strategy. Acta Laboratorium Animalis Scientia Sinica, 22(2):95-102. (in Chinese)
    Peng YZ, Ye ZZ, Zou RJ, Wang YX, Tian BP, Ma YY, Shi LM. 1991. Biology of Chinese Tree Shrews. Kunming:Yunnan Science and Technology Press. (in Chinese)
    Sun YM, Yang JZ, Sun HY, Ma YY, Wang JH. 2012. Establishment of tree shrew chronic morphine dependent model. Zoological Research, 33(1):14-18. (in Chinese)
    Tsang WN, Collins PM. 1985. Techniques for hand-rearing Tree-shrews (Tupaia belangeri) from birth. Zoo Biology, 4(1):23-31.
    van Soom A, Boerjan ML, Bols PE, Vanroose G, Lein A, Coryn M, de Kruif A. 1997. Timing of compaction and inner cell allocation in bovine embryos produced in vivo after superovulation. Biology of Reproduction, 57(5):1041-1049.
    Wang J, Zhou QX, Lü LB, Xu L, Yang YX. 2012. A depression model of social defeat etiology using tree shrews. Zoological Research, 33(1):92-98. (in Chinese)
    Xia HJ, Wang CY, Zhang HL, He BL, Jiao JL, Chen CS. 2012. Characteri-zation of spontaneous breast tumor in tree shrews (Tupaia belangeri chinenesis). Zoological Research, 33(1):55-59. (in Chinese)
    Xu L, Zhang Y, Liang B, Lü LB, Chen CS, Chen YB, Zhou JM, Yao YG. 2013. Tree shrews under the spot light:emerging model of human diseases. Zoological Research, 34(2):59-69. (in Chinese)
    Yang ZF, Zhao J, Zhu YT, Wang YT, Liu R, Zhao SS, Li RF, Yang CG, Li JQ, Zhong NS. 2013. The tree shrew provides a useful alternative model for the study of influenza H1N1 virus. Virology Journal, 10(1):111-119.
    Yue HF, Su JJ, Li Y, Huang YJ, Yang C, Ban KC, Ou C, Cao J, Duan XX, Zhai DM. 2004. Study on in vitro maturation and fertilization of Tupaia glis oocytes. Heilongjiang Journal of Animal Reproduction, 12(1):5-8. (in Chinese)
    Zheng YT, Yao YG, Xu L. 2014. The Basic Biology and Disease Models of Tree Shrews. Kunming:Yunnan Science and Technology Press. (in Chinese)
  • Relative Articles

    [1] Shu Wei, Hai-Rong Hua, Qian-Quan Chen, Ying Zhang, Fei Chen, Shu-Qing Li, Fan Li, Jia-Li Li. Dynamic changes in DNA demethylation in the tree shrew (Tupaia belangeri chinensis) brain during postnatal development and aging. Zoological Research, 2017, 38(2): 96-102.  doi: 10.24272/j.issn.2095-8137.2017.013
    [2] Jian-Ping LI, Yun LIAO, Ying ZHANG, Jing-Jing WANG, Li-Chun WANG, Kai FENG, Qi-Han LI, Long-Ding LIU. Experimental infection of tree shrews(Tupaia belangeri) with Coxsackie virus A16. Zoological Research, 2014, 35(6): 485-491.  doi: 10.13918/j.issn.2095-8137.2014.6.485
    [3] Gui LI, Ren LAI, Gang DUAN, Long-Bao LYU, Zhi-Ye ZHANG, Huang LIU, Xun XIANG. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews. Zoological Research, 2014, 35(6): 492-499.  doi: 10.13918/j.issn.2095-8137.2014.6.492
    [4] Ling XU, Yu FAN, Xue-Long JIANG, Yong-Gang YAO. Molecular evidence on the phylogenetic position of tree shrews. Zoological Research, 2013, 34(2): 70-76.  doi: 10.3724/SP.J.1141.2013.02070
    [5] Xiao-Yun WU, Yun-Hai LI, Qing CHANG, Lin-Qiang ZHANG, Sha-Sha LIAO, Bin LIANG. Streptozotocin induction of type 2 diabetes in tree shrew. Zoological Research, 2013, 34(2): 108-115.  doi: 10.3724/SP.J.1141.2013.02108
    [6] LI Sheng-An, LEE Wen-Hui, ZHANG Yun. Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents. Zoological Research, 2012, 33(1): 1-6.  doi: 10.3724/SP.J.1141.2012.01001
    [7] ZHANG Yuan-Xu, PING Shu-Huang, YANG Shi-Hua. Morphological characteristics and cryodamage of Chinese tree shrew (Tupaia belangeri chinensis) sperm. Zoological Research, 2012, 33(1): 29-36.  doi: 10.3724/SP.J.1141.2012.01029
    [8] SUN Yong-Mei, YANG Jian-Zhen, SUN Hua-Ying, MA Yuan-Ye, WANG Jian-Hong. Establishment of tree shrew chronic morphine dependent model. Zoological Research, 2012, 33(1): 14-18.  doi: 10.3724/SP.J.1141.2012.01014
    [9] WANG Jing, ZHOU Qi-Xin, TIAN Men, YANG Yue-Xiong, XU Lin. Tree shrew models: A chronic social defeat model of depression and a one-trial captive conditioning model of learning and memory. Zoological Research, 2011, 32(1): 24-30.  doi: 10.3724/SP.J.1141.2011.01024
    [10] MA Xu-Tong, LI Fu-Lin, JIANG Hong-Jun, LI Wen-Hui, ZHANG Yun, DU Ting-Yi. Detection and comparison of physiological indexes in the wild and laboratory tree shrew. Zoological Research, 2011, 32(1): 4-10.  doi: 10.3724/SP.J.1141.2011.01004
    [11] ZHANG Ding, GAO Li, ZHANG Yuan-xu, SUN Li, FENG Yue, HE You-wen, XIA Xue-shan, ZHAN. Crucial Factors for de novo Establishment of Long-term Primary Culture of Tree Shrew Hepatocytes. Zoological Research, 2009, 30(1): 24-30.  doi: 10.3724/SP.J.1141.2009.01024
    [12] YANG Min, ZHANG Chi-yu, BEN Kun-long. Cloning and Sequence Analysis of Cyclin T1 cDNA from Tree Shrew (Tupaia belangeri). Zoological Research, 2003, 24(3): 205-210.
    [13] ZHANG Li, BEN Kun-Long. In vitro Infection of Tree Shrew Immunocytes with Human Immunodeficiency Virus Type Ⅰ. Zoological Research, 2001, 22(1): 33-40.
    [14] ZHENG Zi-xiu, ZHONG Jin-yan. Studies on The Lactate Dehydrogenase Isoenzymes of Tree Shrew (Tupaia belangeri chinensis) Tissues:An Electrophoretic Analysis on The Agarose Gel ). Zoological Research, 1991, 12(1): 85-91.
    [15] CAO Xiao-mei. Microscopic Structure of Ovary and Ovarian Activity of Different Seasons in Tree Shrew (Tupaia belangeri chinensis). Zoological Research, 1990, 11(1): 17-24.
    [16] YE Zhi-zhang, PENG Yen-zhang, PAN Ru-liang, WANG Hong. Arterial System in Chinese Tree Shrew (Tupaia belangeri chinensis). Zoological Research, 1990, 11(2): 131-138.
    [17] ZOU Ru-jin, TIAN Bao-pin, JI Wei-zhi, LI Rui-yuan. Research on The Piebaldism of Tree Shrews Breeding in The Captivity. Zoological Research, 1989, 10(zk): 68-78.
    [18] CAO Xiao-mei. Seasonal Changes in Spermatogenesis of Tree Shrew (Tupaia Belangeri Chinensis). Zoological Research, 1989, 10(1): 15-21.
    [19] MA Cai-xia, MA Kun, SHI Li-ming. Electron Microscopic Observations on The Synaptonemal Complex in Spermatocytes of The Tree Shrew (Tupaiabelangeri Chinensis). Zoological Research, 1989, 10(zk): 29-33.
    [20] ZHANG Ya-ping, ZHANG Bing, SHI Li-ming. Restriction Maps of Mitochondrial DNA of Slow Loris and Tree Shrew. Zoological Research, 1989, 10(zk): 79-89.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (969) PDF downloads(1389) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint