Volume 37 Issue 2
Mar.  2016
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Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 2016, 37(2): 96-102. doi: 10.13918/j.issn.2095-8137.2016.2.96
Citation: Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 2016, 37(2): 96-102. doi: 10.13918/j.issn.2095-8137.2016.2.96

Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis

doi: 10.13918/j.issn.2095-8137.2016.2.96
Funds:  This project was supported by the Program for the National Natural Science Foundation of China (31372555), Zhejiang Provincial Natural Science Foundation of China (LZ13C190001), Scientific Research Foundation of Graduate School of Ningbo University (G15063), and KC Wong Magna Fund in Ningbo University
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  • Corresponding author: Jiong CHEN
  • Received Date: 2015-10-22
  • Rev Recd Date: 2015-12-11
  • Publish Date: 2016-03-18
  • Colony-stimulating factor 1 receptor (CSF-1R) is an important regulator of monocytes/macrophages (MO/MΦ). Although several CSF-1R genes have been identified in teleosts, the precise role of CSF- 1R in ayu (Plecoglossus altivelis) remains unclear. In this study, we characterized the CSF-1R homologue from P. altivelis, and named it PaCSF-1R. Multiple sequence alignment and phylogenetic tree analysis showed that PaCSF-1R was most closely related to that of Japanese ricefish (Oryzias latipes). Tissue distribution and expression analysis showed that the PaCSF-1R transcript was mainly expressed in the head kidney-derived MO/MΦ, spleen, and head kidney, and its expression was significantly altered in various tissues upon Vibrio anguillarum infection. After PaCSF-1R neutralization for 48 h, the phagocytic activity of MO/MΦ was significantly decreased, suggesting that PaCSF-1R plays a role in regulating the phagocytic function of ayu MO/MΦ.
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Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis

doi: 10.13918/j.issn.2095-8137.2016.2.96
Funds:  This project was supported by the Program for the National Natural Science Foundation of China (31372555), Zhejiang Provincial Natural Science Foundation of China (LZ13C190001), Scientific Research Foundation of Graduate School of Ningbo University (G15063), and KC Wong Magna Fund in Ningbo University
    Corresponding author: Jiong CHEN

Abstract: Colony-stimulating factor 1 receptor (CSF-1R) is an important regulator of monocytes/macrophages (MO/MΦ). Although several CSF-1R genes have been identified in teleosts, the precise role of CSF- 1R in ayu (Plecoglossus altivelis) remains unclear. In this study, we characterized the CSF-1R homologue from P. altivelis, and named it PaCSF-1R. Multiple sequence alignment and phylogenetic tree analysis showed that PaCSF-1R was most closely related to that of Japanese ricefish (Oryzias latipes). Tissue distribution and expression analysis showed that the PaCSF-1R transcript was mainly expressed in the head kidney-derived MO/MΦ, spleen, and head kidney, and its expression was significantly altered in various tissues upon Vibrio anguillarum infection. After PaCSF-1R neutralization for 48 h, the phagocytic activity of MO/MΦ was significantly decreased, suggesting that PaCSF-1R plays a role in regulating the phagocytic function of ayu MO/MΦ.

Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 2016, 37(2): 96-102. doi: 10.13918/j.issn.2095-8137.2016.2.96
Citation: Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 2016, 37(2): 96-102. doi: 10.13918/j.issn.2095-8137.2016.2.96
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